LOCUS       sequence-222507-        8553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High expression of mCherry-EGFP-PIM. Can be used for inducible 
            aggregate formation upon AP20187 addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, 
            Kapitein LC
  TITLE     Probing aggrephagy using chemically-induced protein aggregates.
  JOURNAL   Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.
  PUBMED    30315152
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-12"; volume: "9(1):4245. doi"; pages: " 
            10.1038/s41467-018-06674-4"
COMMENT     Sequence Label: pGW1-mCherry-EGFP-PIM
FEATURES             Location/Qualifiers
     source          1..8553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     774..794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     820..844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          959..1785
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             1827..2147
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2154..2474
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVEHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDCAYGATGHPGIIPPHTTLVFDVELL
                     KLE"
     CDS             2490..3194
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDVLSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2632..2650)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2900..2919
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(3204..3206,3207..3917)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3204..3206,3207..3917)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3246..3267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3507..3526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3854..3875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4608..4928
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4935..5255
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4935..5255
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    5380..5514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5417..5436)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5471..5490
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5537..5553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5537..5553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5550..5572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5561..5577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5585..5591,5592..5609,5610..5615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5767..5784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5938..6526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6018..6037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6697..7488,7489..7557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7320..7339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7767..7785
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7823..7845)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7945..7964
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8196..8218
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8210..8227
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8211..8227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcgcagttt cgatataggt gacagacgat atgaggctat atcgccgata gaggcgacat
       61 caagctggca catggccaat gcatatcgat ctatacattg aatcaatatt ggccattagc
      121 catattattc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
      181 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      841 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      901 cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtgacgt
      961 aagtaccgcc tatagagtct ataggcccac ccccttggct tcttatgcat gctatactgt
     1021 ttttggcttg gggtctatac acccccgctt cctcatgtta taggtgatgg tatagcttag
     1081 cctataggtg tgggttattg accattattg accactcccc tattggtgac gatactttcc
     1141 attactaatc cataacatgg ctctttgcca caactctctt tattggctat atgccaatac
     1201 actgtccttc agagactgac acggactctg tatttttaca ggatggggtc tcatttatta
     1261 tttacaaatt cacatataca acaccaccgt ccccagtgcc cgcagttttt attaaacata
     1321 acgtgggatc tccacgcgaa tctcgggtac gtgttccgga catgggctct tctccggtag
     1381 cggcggagct tctacatccg agccctgctc ccatgcctcc agcgactcat ggtcgctcgg
     1441 cagctccttg ctcctaacag tggaggccag acttaggcac agcacgatgc ccaccaccac
     1501 cagtgtgccg cacaaggccg tggcggtagg gtatgtgtct gaaaatgagc tcggggagcg
     1561 ggcttgcacc gctgacgcat ttggaagact taaggcagcg gcagaagaag atgcaggcag
     1621 ctgagttgtt gtgttctgat aagagtcaga ggtaactccc gttgcggtgc tgttaacggt
     1681 ggagggcagt gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag
     1741 ctgacagact aacagactgt tcctttccat gggtcttttc tgcagtcacc gtccttgaca
     1801 cgaagcttgc caccatgcgc tctagaggcg tccaagtcga aaccattagt cccggcgatg
     1861 gcagaacatt tcctaaaagg ggacaaacat gtgtcgtcca ttatacaggc atgttggagg
     1921 acggcaaaaa ggtggacagt agtagagatc gcaataaacc tttcaaattc atgttgggaa
     1981 aacaagaagt cattagggga tgggaggagg gcgtggctca aatgtccgtc ggccaacgcg
     2041 ctaagctcac catcagcccc gactacgcat acggcgctac cggacatccc ggaattattc
     2101 cccctcacgc taccttggtg tttgacgtcg aactgttgaa gctcgagact agaggagtgc
     2161 aggtggagac tatctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg
     2221 tggagcacta caccgggatg cttgaagatg gaaagaaagt tgattcctcc cgggacagaa
     2281 acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg
     2341 ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tgtgcctatg
     2401 gtgccactgg gcacccaggc atcatcccac cacataccac tctcgtcttc gatgtggagc
     2461 ttctaaaact ggaaactagt gaaccggtag tgagcaaggg cgaggaggat aacatggcca
     2521 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     2581 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     2641 aggtgaccaa gggtggcccc ctgcccttcg cctgggacgt cctgtcccct cagttcatgt
     2701 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     2761 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     2821 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     2881 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     2941 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     3001 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     3061 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     3121 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3181 acgagctgta caagggcgcg cctgtgagca agggcgagga gctgttcacc ggggtggtgc
     3241 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3301 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3361 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     3421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3481 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3541 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3601 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     3661 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     3721 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3781 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     3841 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     3901 tggacgagct gtacaaggct agccgctcac gcggcgttca ggtggagact ataagcccgg
     3961 gggatggtcg cacttttcct aaacgaggcc agacgtgcgt agttcactat accggcatgc
     4021 ttgaagacgg caagaaggtg gatagctcta gggaccggaa taagcccttc aagttcatgc
     4081 ttggtaagca agaagtgatc cggggttggg aagagggggt tgcccaaatg tcagtcggac
     4141 aacgggcgaa gttgactatt tctcctgatt acgcttatgg tgcgacggga catcctggca
     4201 tcataccgcc tcacgccact cttgtctttg atgtggaact tttgaagctg gaaacgcgag
     4261 gggttcaagt tgagaccatt tcaccgggcg atggcagaac attcccgaaa aggggacaaa
     4321 cgtgtgtagt acactatacg ggcatgctcg aagacgggaa aaaggttgac tcaagtagag
     4381 atcggaacaa gcctttcaag ttcatgttgg ggaaacaaga ggtaatacgc gggtgggagg
     4441 agggagtcgc tcagatgtct gtaggccaaa gggcgaagct caccatatca ccagattacg
     4501 cttacggggc gacgggccat cccggaataa tacctccgca cgcgacactt gtttttgatg
     4561 ttgaattgct taagttggag accagcagat ctgtcgaccg ctctagagga gtgcaggtgg
     4621 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc
     4681 actacaccgg gatgcttgaa gatggaaaga aatttgactc ctcccgggac agaaacaagc
     4741 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc
     4801 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca
     4861 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa
     4921 aactggaaac tagaggcgtg caggtcgaga ccatcagccc cggcgacggc cgcacctttc
     4981 ccaagagagg ccagacttgc gtggtccact acaccggcat gctggaggac ggcaagaagt
     5041 tcgacagcag ccgcgaccgc aacaagccct tcaagttcat gctgggcaaa caggaagtga
     5101 tccgcggctg ggaggaaggc gtggctcaga tgagcgtggg gcagcgggcc aagctgacca
     5161 tcagccccga ctatgcctac ggcgccaccg gccaccccgg catcatcccc ccccacgcca
     5221 ccctggtgtt cgacgtggag ctgctgaagc tggagactag ttaggaattc attgatcata
     5281 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     5341 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     5401 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5461 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgtacc
     5521 gagctcgcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5641 aactacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7561 cttccttttt caataagcgg ccgcggccat gccggccact agtctcgagt tattattgaa
     7621 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7681 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     7741 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     7801 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7861 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7921 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7981 tgaagacgtc gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     8041 ctgcataaat aaaaaaaatt agtcagggcg ccattcgcca ttcaggctgc gcaactgttg
     8101 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     8161 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     8221 ggccagtgcc cgtgtcaagg acggtgactg cagtgaataa taaaatgtgt gtttgtccga
     8281 aatacgcgtt ttgagatttc tgtcgccgac taaattcatg tcgcgcgata gtggtgttta
     8341 tcgccgatag agatggcgat attggaaaaa tcgatatttg aaaatatggc atattgaaaa
     8401 tgtcgccgat gtgagtttct gtgtaactga tatcgccatt tttccaaaag tgatttttgg
     8461 gcatacgcga tatctggcga tagcgcttat atcgtttacg ggggatggcg atagacgact
     8521 ttggtgactt gggcgattct gtgtgtcgca aat
//
