LOCUS       sequence-222516-         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for high level expression in mycobacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Ainsa 
            JA, Hansen EJ, Gumbo T, van Oers NS
  TITLE     Mycobacterial shuttle vectors designed for high-level protein 
            expression in infected macrophages.
  JOURNAL   Appl Environ Microbiol. 2012 Oct;78(19):6829-37. doi: 
            10.1128/AEM.01674-12. Epub 2012 Jul 20.
  PUBMED    22820329
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.01674-12"; journalName: "Appl Environ Microbiol"; date:
            "2012-10"; volume: "78"; issue: "19"; pages: "6829-37"
COMMENT     Sequence Label: pSUM36-MCS2-myc
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..101
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(130..146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(130..146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(143..165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(178..184,185..202,203..208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    223..244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(481..498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccagtgcca agctgcgagc tcgaattctg attaacttta taaggaggaa aaacatatga
       61 cagaagcttt ggaacaaaaa cttatttctg aagaagatct gtgaggatcc ccgggaattc
      121 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      181 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      241 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      301 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct agcggagtgt
      361 atatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
      421 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
      481 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cnagcggaaa tggcttacga
      541 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
      601 gcggcaaagc cgtttttc
//
