LOCUS       sequence-222518-       11461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 11461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti GW-NL-myc
FEATURES             Location/Qualifiers
     source          1..11461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     668..688
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             731..911
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    958..1083
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1576..1809
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1994..2038
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2336..2453
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2518..3729
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3566..3585
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3761..3885
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3910..3915,3916..3933,3934..3940)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3994..4674
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(4060..4079)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4994..5299
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5178..5197
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5340..5464)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5472..5981
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             5982..6011
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    6049..6637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6102..6122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6520..6531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6640..6656)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6641..6657)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7162..7342
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7368..7385)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7374..7598
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7644..8072
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7731..7750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7941..7962
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8081..8101)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8086..8415
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8266..8401
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8328..8347
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8463..8510
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8529..8903
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9033..9154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9070..9089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9124..9143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9203..9219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9203..9219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9216..9238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9227..9243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9251..9257,9258..9275,9276..9281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9296..9317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9434..9451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9605..10193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9685..9704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10364..11155,11156..11224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10987..11006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11225..11329)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11404..11423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
       61 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
      121 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      541 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      601 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      661 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      721 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      781 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      841 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      901 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      961 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1021 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1081 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1141 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1201 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1261 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1321 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1381 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1441 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1501 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1561 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1621 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1681 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1741 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1801 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1861 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1921 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1981 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2041 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2101 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2161 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2221 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2281 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2341 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2401 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2461 attacaggga cagcagagat ccagtttggt taattaaccc gtgtcggctc cagatctggc
     2521 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2581 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2641 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2701 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2761 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2821 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2881 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
     2941 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
     3001 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
     3061 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
     3121 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
     3181 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
     3241 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     3301 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
     3361 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
     3421 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
     3481 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     3541 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     3601 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     3661 tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt
     3721 ttgttagacg aagcttgggc ccgggaatta attcgctagc acaagtttgt acaaaaaagc
     3781 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     3841 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     3901 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     3961 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     4021 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     4081 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     4141 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     4201 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     4261 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     4321 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     4381 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     4441 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     4501 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     4561 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     4621 cagtactgcg atgagtggca ggcggggcgt aatctagagg atccggctta ctaaaagcca
     4681 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     4741 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     4801 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     4861 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     4921 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     4981 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     5041 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     5101 ggccagtgca cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat
     5161 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     5221 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     5281 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     5341 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     5401 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     5461 tggtgaagct tgtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct
     5521 acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg
     5581 tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca
     5641 tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa
     5701 tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca
     5761 cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag
     5821 gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca
     5881 aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca
     5941 acggagtgac cggctggcgg ctgtgcgaac gcattctggc ggaacagaaa ctgatctctg
     6001 aagaagacct gtgactcgag tctagaattc gatatcaagc ttatcgataa tcaacctctg
     6061 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6121 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6181 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6241 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6301 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6361 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6421 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6481 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6541 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6601 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     6661 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     6721 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     6781 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6841 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     6901 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     6961 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     7021 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7081 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     7141 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7201 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7261 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7321 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     7381 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7441 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7501 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7561 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7621 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7681 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7741 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7801 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7861 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7921 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7981 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     8041 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     8101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8161 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8221 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8281 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8341 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8401 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     8461 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     8521 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     8581 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     8641 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     8701 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     8761 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     8821 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     8881 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     8941 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     9001 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     9061 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9121 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     9181 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     9241 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     9301 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9361 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9421 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9481 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9541 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9781 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9841 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9901 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9961 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    10021 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    10081 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    10141 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    10201 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    10261 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    10321 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10381 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10441 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10501 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10561 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10621 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10681 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10741 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10801 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10861 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10921 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10981 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11041 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    11101 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    11161 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    11221 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11281 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11341 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
    11401 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
    11461 g
//
