LOCUS       sequence-22253-e         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2M
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..104
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(108..116,117..131)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aataattttt gtttaacttt aagaaggaga atatacatat gcggggttct catcatcatc
       61 atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
      121 atgacgataa ggatccgagc tcgagatctg cagatcaaac aagtttgtac aaaaaagcag
      181 gctccgcggc cgcccccttc accatgatca agctgttctc gctgaagcag cagaagaagg
      241 aggaggagtc ggcgggcggc accaagggca gcagcaagaa ggcgtcggcg gcgcagctgc
      301 ggatccagaa ggacataaac gagctgaacc tgcccaagac gtgtgatatc agcttctcag
      361 atccagacga cctcctcaac ttcaagctgg tcatctgtcc tgatgagggc ttctacaaga
      421 gtgggaagtt tgtgttcagt tttaaggtgg gccagggtta cccgcatgat ccccccaagg
      481 tgaagtgtga gacaatggtc tatcacccca acattgacct cgagggcaac gtctgcctca
      541 acatcctcag agaggactgg aagccagtcc ttacgataaa ctccataatt tatggcctgc
      601 agtatctctt cttggagccc aaccccgagg acccactgaa c
//
