LOCUS       sequence-222536-        6427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Kendall SD, Linardic CM, Adam SJ, Counter CM
  TITLE     A network of genetic events sufficient to convert normal human cells
            to a tumorigenic state.
  JOURNAL   Cancer Res. 2005 Nov 1. 65(21):9824-8.
  PUBMED    16267004
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2005 Nov 1. 65(21):9824-8."
COMMENT     Sequence Label: pbabe-c-mycT58A+HRasG12V
FEATURES             Location/Qualifiers
     source          1..6427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             44..513
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    578..777
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             978..1394
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1324..1346
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1362..1378
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2870..2890)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2875..3204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3055..3190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(3217..3249,3250..3252,3253..3786)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     LTR             3957..4426
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4556..4573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4727..5315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4807..4826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5475..6266,6267..6335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6098..6117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6336..6427,1..13))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac
       61 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
      421 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
      481 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
      541 cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt
      601 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
      661 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
      721 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
      781 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
      841 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
      901 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
      961 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
     1021 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
     1081 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
     1141 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
     1201 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
     1261 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
     1321 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
     1381 cttctctagg cgccggccgg atcccagtgt ggtggtacgc ttgcatgcct gcaggtcgag
     1441 ctgtttgaag gctggatttc ctttgggcgt tggaaacccc gcagacagcc acgacgatgc
     1501 ccctcaacgt gaacttcacc aacaggaact atgacctcga ctacgactcc gtacagccct
     1561 atttcatctg cgacgaggaa gagaatttct atcaccagca acagcagagc gagctgcagc
     1621 cgcccgcgcc cagtgaggat atctggaaga aattcgagct gcttcccgcc ccgcccctgt
     1681 ccccgagccg ccgctccggg ctctgctctc catcctatgt tgcggtcgct acgtccttct
     1741 ccccaaggga agacgatgac ggcggcggtg gcaacttctc caccgccgat cagctggaga
     1801 tgatgaccga gttacttgga ggagacatgg tgaaccagag cttcatctgc gatcctgacg
     1861 acgagacctt catcaagaac atcatcatcc aggactgtat gtggagcggt ttctcagccg
     1921 ctgccaagct ggtctcggag aagctggcct cctaccaggc tacgcgcaaa gacagcacca
     1981 gcctgagccc cgcccgcggg cacagcgtct gctccacctc cagcctgtac ctgcaggacc
     2041 tcaccgccgc cgcgtccgag tgcattgacc cctcagtggt ctttccctac ccgctcaacg
     2101 acagcagctc gcccaaatcc tgtacctcgt ccgattccac ggccttctct ccttcctcgg
     2161 actcgctgct gtcctccgag tcctccccac gggccagccc tgagccccta gtgctgcatg
     2221 aggagacacc gcccaccacc agcagcgact ctgaagaaga gcaagaagat gaggaagaaa
     2281 ttgatgtggt gtctgtggag aagaggcaaa cccctgccaa gaggtcggag tcgggctcat
     2341 ctccatcccg aggccacagc aaacctccgc acagcccact ggtcctcaag aggtgccacg
     2401 tctccactca ccagcacaac tacgccgcac ccccctccac aaggaaggac tatccagctg
     2461 ccaagagggc caagttggac agtggcaggg tcctgaagca gatcagcaac aaccgcaagt
     2521 gctccagccc caggtcctca gacacggagg aaaacgacaa gaggcggaca cacaacgtct
     2581 tggaacgtca gaggaggaac gagctgaagc gcagcttttt tgccctgcgt gaccagatcc
     2641 ctgaattgga aaacaacgaa aaggccccca aggtagtgat cctcaaaaaa gccaccgcct
     2701 acatcctgtc cattcaagca gacgagcaca agctcacctc tgaaaaggac ttattgagga
     2761 aacgacgaga acagttgaaa cacaaactcg aacagcttcg aaactctcgt gcataaactg
     2821 acctaactcg actctagtag gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt
     2881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3121 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3181 tttggaggcc taggcttttg caaaaagctt gccaccatga cggaatataa gctggtggtg
     3241 gtgggcgccg tcggtgtggg caagagtgcg ctgaccatcc agctgatcca gaaccatttt
     3301 gtggacgaat acgaccccac tatagaggat tcctaccgga agcaggtggt cattgatggg
     3361 gagacgtgcc tgttggacat cctggatacc gccggccagg aggagtacag cgccatgcgg
     3421 gaccagtaca tgcgcaccgg ggagggcttc ctgtgtgtgt ttgccatcaa caacaccaag
     3481 tcttttgagg acatccacca gtacagggag cagatcaaac gggtgaagga ctcggatgac
     3541 gtgcccatgg tgctggtggg gaacaagtgt gacctggctg cacgcactgt ggaatctcgg
     3601 caggctcagg acctcgcccg aagctacggc atcccctaca tcgagacctc ggccaagacc
     3661 cggcagggag tggaggatgc cttctacacg ttggtgcgtg agatccggca gcacaagctg
     3721 cggaagctga accctcctga tgagagtggc cccggctgca tgagctgcaa gtgtgtgctc
     3781 tcctgataaa tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     3841 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     3901 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
     3961 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     4021 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     4081 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     4141 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     4201 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     4261 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
     4321 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     4381 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
     4441 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
     4501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4621 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4681 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4741 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4801 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4861 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4921 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4981 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5041 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5101 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5161 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5221 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5281 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5341 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5401 aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat atatgagtaa
     5461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5761 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6061 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6421 aaacaaa
//
