LOCUS       sequence-22254-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2F
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..93
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(97..105,106..120)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    148..172
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttaacttta agaaggagat atacatatag cggggttctc atcatcatca tcatcatggt
       61 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
      121 gatccgagct cgagatctgc agatcaaaca agtttgtaca aaaaagttgg catgctaacg
      181 ctagcaagta aactgaagcg tgacgatggt ctcaaagggt cccggacggc agccacagcg
      241 tccgactcga ctcggagggt ttctgtgaga gacaaattgc ttgttaaaga ggttgcagaa
      301 cttgaagcta atttaccttg tacatgtaaa gtgcattttc ctgatccaaa caagcttcat
      361 tgttttcagc taacagtaac cccagatgag ggttactacc agggtggaaa atttcagttt
      421 gaaactgaag ttcccgatgc gtacaacatg gtgcctccca aagtgaaatg cctgaccaag
      481 atctggcacc ccaacatcac agagacaggg gaaatatgtc tgagtttatt gagagaacat
      541 tcaattgatg gcactggctg ggctcccaca agaacattaa aggatgtcgt ttggggatta
      601 aactctttgt ttactgatct tttgaatttt gatgatccac tgaatattga agctgcagaa
      661 cat
//
