LOCUS       sequence-22255-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2Q2
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..106
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(110..118,119..133)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcnnnannnn tttgtttaac tttaagaagg agatatacat atgcggggtt ctcatcatca
       61 tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga
      121 cgatgacgat aaggatccga gctcgagatc tgcagatcaa acaagtttgt acaaaaaagc
      181 aggctccgcg gccgccccct tcaccatgtc cgtgtcaggg ctcaaggccg agctgaagtt
      241 cctggcgtcc atcttcgaca agaaccacga gcgattccgc atcgtcagtt ggaagctgga
      301 cgagctgcac tgccagttcc tggtgccgca gcagggcagc ccgcactcgc tgccgccgcc
      361 actcacgctc cactgcaaca tcacggaatc ctatccatct tcttcaccga tatggtttgt
      421 ggattctgaa gacccaaatc tgacatcagt tctggaacgt ctagaagata ctaagaacaa
      481 caatttgctt cgtcagcaat tgaagtggtt gatatgtgaa ctctgcagtt tatataacct
      541 tcctaagcac ctggatgttg agatgctaga tcaaccacta cccacgggtc agaatgggac
      601 aacagaagaa gtgacttcag aagaannnna naaaaaaaaa anaangnngn tgaaanaann
      661 nnnnagantt nnnnnnctat gagatgaagg aagaagagcc tattagtggg aaaaagtcag
      721 aggatgaann attgaaaaag aaaatttggc aatattagag aaaattagga agactcaaag
      781 gcaagaccat ttaaatggtg cagtgtctgg gtcagtgcaa g
//
