LOCUS       sequence-222556-        3138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Nakanishi K, Weinberg DE, Bartel DP, Patel DJ
  TITLE     Structure of yeast Argonaute with guide RNA.
  JOURNAL   Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211.
  PUBMED    22722195
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-06-20- 20"; volume: "486"; issue: "7403"; 
            pages: "368-74"
COMMENT     Sequence Label: pRSF-KpAGO207-1251
FEATURES             Location/Qualifiers
     source          1..3138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgagaaaa ctgtagatca acctaaggaa gatactcatg caatctataa agttgaaaat
       61 agacatgatt atggtactaa aggtactaaa gttgacattt tgacaaacca tattttgctt
      121 gcggtaggta acgatgttcc tactgaaaaa atcgacaaag aattggttcc taaattagat
      181 ggttggtgga aaactgcctt catctttact tatcatatcg atttcaaacc ccaacaaaaa
      241 ggccctccac gtagaggcaa accagttcca ccacaggaat tatcaaagcc aaagaaatat
      301 gaattaattg aagctttact ggatgaagat gaaatcttgt acaagtatag ggatcgtatc
      361 gctttcaatg gtgaagatac tatttattct catgtgccac tagaagaatt cactttgttt
      421 gatggttgtt gggaagtcag taacaagcaa aagaagaagg ttgttccagg tatgggtgct
      481 ccatccaaca aagcttctct acaaaaaaaa atagatccag aattagaaga aatggtttct
      541 caaatcactt tgaaattcag tggtaaagta ggcttgaaag atatttataa cgacaccact
      601 actcaagaca ccgaagtaca ggaaagtaga atgtctgcaa tcgataagac atgtttgcta
      661 tctttattag gtgctaagtt catgagtaca gatgatttga ttttccaagt tcaaggtaac
      721 aagttcttta ttttcaataa ttttgctaaa gctatcccat tccaaatcgg tggttatttg
      781 ttgcagggtt ttactgtttc attaactcat gtttatggtg gtgtcgcttt aaacactgtc
      841 agtgttcctg ctccatttat taaacatacc aagtacttgc caggtgatcc aagatttaaa
      901 aacaatgaaa aggaacaatt tacattaatg gactggatca ttgaatgtta ccaccaatct
      961 aaggctataa gagatatcag atataatcca aaaacagctc ctccaccatc agttaaagat
     1021 ttgaactatt ttgtcgaaaa gaacactgat atttcagctt tgttaaaggg tttaaaggtt
     1081 tacagaccgt acatcaatta tagtatcaat aaagatggta ccccaaaacc accaagaaag
     1141 agatcttcta agggtattgt tggatttacc cgtgaatctg cggtatccat gaggtttaat
     1201 gttcttgaaa gtagtttgaa gaagaacagt gctccaaaac ctaatgaaaa acctatcaat
     1261 atcaatacta ttgattattt caagaggaaa tatgacatta ctttgaaata tcctgatatg
     1321 aagttagtaa acttgggcgg taaaaatgat gtcgttcctc ctgaatgttt gactattgtg
     1381 ccgggtcaaa aattgaaggg tcaaattttt gatacaaaaa cttatatcga cttcagtgca
     1441 attagaccaa ctgaaaagtt tgatttaatc tccaggttat ctatgccagc tataaaaaga
     1501 gggttaactg attctgaaaa ggaagaatca tcagctcctc acaatagtgc ttatcaattt
     1561 atgagagtac catctcgtat tctagatgcc ccagttgttc agttcaaaga atctaccttt
     1621 gaatataagg acaagagtta tggaactaag catgaagaat ctaaaggtaa ctggaacatg
     1681 aaaggtcacc aatttatttc cactccagcc aaacaggtca acttaagagc aatatttatt
     1741 aataatgcta acacagctcc accagcatct atggaaagtg aacttgacat ctctatggat
     1801 aaattcgcat ctgatgttaa acaattaggt gtggacttca acgtatcagg taaaccaatt
     1861 ctaattaatc aatttggtcc cccaattaag aaattccaag gtggtggccg tggtggccgt
     1921 ggtggtcgtg gtagccgtgg tggccgtggt ggccgtggtg ctccatctgg tcctccaact
     1981 ttcgaaacct ctccaggtga gatatctttg ttaaacttat tagaaaatat tccaagcaat
     2041 acctatattt tgtatgtatt gcgccgtggt aacgattctg ctgtttatga tagattgaaa
     2101 tatatcactg atttgaaatt tggtgcattg aattcctgtg ttgtttggga caacttcaaa
     2161 aagaattcta ttcaatataa ttccaatgtt gttatgaaga tgaacttgaa gttattaggt
     2221 agtaaccact ctctatctat tgaaaacaac aaactattaa ttgataagga atctaacttg
     2281 ccaatattag tgttgggttc tgatgtgaca cattatcctg aaaaggatca aaactctatt
     2341 gcctcgttag taggttcata cgatgacaaa tttacccaat tccctggtga ttacatgctt
     2401 caagatggtc caggtgaaga aataattact aatgtcggtt cattaatgtt gaacagatta
     2461 aagatatatc aaaaacataa taatggtaaa ttaccaacga agatcatgta cttcagagat
     2521 ggtgtttcag ttgaccaatt ctctcaagtt gttaagattg aagttaagtc tattaaggaa
     2581 tcagtccgta aatttggtcc tcaattaaat ggtggtaaca aatacgatcc accagttaca
     2641 tgtattgcca ctgtcaaaag aaatcaagtc agatttattc ctatccaaga aaatgcgaag
     2701 aatgaaaagg gtgaagaagt tgctgttcaa tccatgggta atgttatgcc aggtactgtt
     2761 gtagaccgtg gtatcacatc tgtggcacac tttgatttct ttatccaatc tcatcaagct
     2821 ttgaagggta ctggtgtccc atgccactat tggtgtctat atgacgaaaa ccaatctact
     2881 tctgactact tacaagaaat atgtaacaac ttatgttaca ttttcggtag atctactacc
     2941 agtgtaaaag tcccagcccc agtatattat gccgatttgt tgtgtacccg tgctacatgc
     3001 tttttcaagg caggttttga acttaatatg gctcaagcac caaaggagaa gggttctaag
     3061 gatcaaccta ctgtttccaa gaatgttcta ttaccacaag ttaacgataa cattaaatcc
     3121 gtaatgtatt acatttga
//
