LOCUS       sequence-22258-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2S
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..94
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(98..106,107..121)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    149..173
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtttaactt taagaaggag atatacatat gcggggttct catcatcatc atcatcatgg
       61 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      121 ggatccgagc tcgagatctg cagatcaaac aagtttgtac aaaaaagcag gctccgcggc
      181 cgcccccttc accatgaact ccaacgtgga gaacctaccc ccgcacatca tccgcctggt
      241 gtacaaggag gtgacgacac tgaccgcaga cccacccgat ggcatcaagg tctttcccaa
      301 cgaggaggac ctcaccgacc tccaggtcac catcgagggc cctgagggga ccccatatgc
      361 tggaggtctg ttccgcatga aactcctgct ggggaaggac ttccctgcct ccccacccaa
      421 gggctacttc ctgaccaaga tcttccaccc gaacgtgggc gccaatggcg agatctgcgt
      481 caacgtgctc aagagggact ggacggctga gctgggcatc cgacacgtac tgctgaccat
      541 caagtgcctg ctgatccacc ctaaccccga gtctgcactc aacgaggagg cgggccgcct
      601 gctcttggag aactacgagg agtatgcggc tcgggcccgt ctgctcacag agatccacgg
      661 gggcgccggc g
//
