LOCUS       sequence-222583-       12699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ATG2A with GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Tamura N, Nishimura T, Sakamaki Y, Koyama-Honda I, Yamamoto H, 
            Mizushima N
  TITLE     Differential requirement for ATG2A domains for localization to 
            autophagic membranes and lipid droplets.
  JOURNAL   FEBS Lett. 2017 Dec;591(23):3819-3830. doi: 10.1002/1873-3468.12901.
            Epub 2017 Nov 20.
  PUBMED    29113029
REFERENCE   2  (bases 1 to 12699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.12901"; journalName: "FEBS Lett"; date: 
            "2017-12"; volume: "591"; issue: "23"; pages: "3819-3830"
COMMENT     Sequence Label: pMRXIP-GFP-hATG2A
FEATURES             Location/Qualifiers
     source          1..12699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    739..1096
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1161..1577
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1507..1529
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1545..1561
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1587..1961
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1899..1915
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1973..1975,1976..1978,1979..2689)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1973..1975,1976..1978,1979..2689)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2018..2039)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2279..2298)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2626..2647
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8622..9188
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8638..9190
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8782..8799)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9009..9028
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9202..9801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9202..9221)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9698..9718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10025..10494
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10624..10641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10795..11383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10875..10894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11547..12338,12339..12407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12170..12189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12408..12512)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12580..12598
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      421 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      481 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      541 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
      601 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
      661 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
      721 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
      781 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
      841 gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc
      901 cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc gatcgttttg
      961 gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga gacgagaacc
     1021 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg
     1081 cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     1141 tgtctgaaaa tatgggcccg ggccagactg ttaccactcc cttaagtttg accttaggtc
     1201 actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt
     1261 gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca
     1321 cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg
     1381 gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc
     1441 cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt
     1501 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
     1561 ctccttctct aggcgccccc atatggccat atgagatctt atatggggca cccccgcccc
     1621 ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc
     1681 acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc
     1741 aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg
     1801 tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc
     1861 tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg
     1921 tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatctg ccatggtgag
     1981 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     2041 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     2101 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     2161 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     2221 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     2281 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     2341 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     2401 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     2461 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2521 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2581 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2641 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gtggcggtgg
     2701 ctcgggcggt ggtgggtcgg gtggcggcgg atctatcgat gaattggaat tcatgtcacg
     2761 atggctgtgg ccatggtcaa actgtgtgaa agagcgggtc tgccgctact tgctgcacca
     2821 ctacttaggt cacttcttcc aagagcacct cagcctggac cagctcagcc tcgatctgta
     2881 caagggcagc gttgccctgc gagacatcca cctggaaatc tggtctgtga acgaggtgct
     2941 ggagtcaatg gagtcaccgc tggagctggt ggaaggcttc gtgggctcca tcgaggtggc
     3001 cgtgccctgg gctgctctgc tcaccgacca ctgcacagtg cgcgtgtccg gcctccagct
     3061 caccttgcag ccccgccggg gtccagcgcc aggggctgcc gactcacaga gctgggcctc
     3121 atgcatgacc acaagcctgc agctggccca ggagtgtctg cgggatgggc taccggagcc
     3181 ctctgagcca ccacagcccc tggaggggct ggagatgttt gcccagacca ttgagactgt
     3241 gcttcggagg atcaaagtga ccttcctgga cactgtcgtg agggtggagc actctccggg
     3301 tgatggggaa cgtggtgtgg ccgtcgaggt ccgtgtgcag agactggagt actgtgatga
     3361 ggcagtgcgg gacccaagcc aggcgccgcc ggtggatgtg catcagccgc ctgccttcct
     3421 gcacaagctg ctgcagctgg caggggtccg cctgcactac gaggagctcc cggcacagga
     3481 agagcctcca gagcccccct tgcagatcgg cagctgctca gggtacatgg agctgatggt
     3541 gaagttgaag caaaatgagg ccttccctgg ccccaagttg gaggtggcgg gacagctggg
     3601 ctccctgcac ctgctcctga ccccgaggca gctccagcaa cttcaggaac tgctcagcgc
     3661 cgtgagcctt acagaccacg agggcctggc tgacaagctg aacaagagcc gcccgctagg
     3721 tgccgaagac ctgtggctga ttgagcagga cctgaaccag cagctgcagg caggggcagt
     3781 ggctgagccc ctcagcccag acccccttac caaccccctt ctcaacctgg ataacactga
     3841 cctcttcttc tccatggctg gcctcacaag cagtgtggcc tcagccctct ctgagctctc
     3901 cctctccgat gtagacctgg cctcctctgt gcgcagcgac atggcctccc gccggctctc
     3961 tgcccaggcc cacccagctg gcaagatggc ccccaacccc ctcctggaca ccatgcgccc
     4021 tgactcgctg ctgaagatga ccttgggggg tgtgaccctg accttgcttc agacgtctgc
     4081 cccatcttcc ggaccacctg acctcgccac gcactttttc accgagtttg atgccaccaa
     4141 ggatgggccc ttcggttccc gagacttcca tcaccttcga ccacgcttcc agagggcctg
     4201 tccctgtagc catgttcggc taacgggcac agccgtgcag ctgtcctggg agctgcggac
     4261 gggcagtcgg ggccggcgga caaccagcat ggaagtgcac ttcgggcagc tggaggtgct
     4321 ggagtgtctg tggccccggg gcacctctga gcctgagtac acggagatcc tgacctttcc
     4381 tggtacgctg ggctcccagg cctcagctcg gccctgcgcc catctgcgcc acacacagat
     4441 cctgcgccgt gtgcctaaga gccgaccccg gcgctcagtt gcctgccatt gccactcaga
     4501 actggccctg gacctggcca acttccaggc ggacgtggag ctgggggccc tggaccggct
     4561 ggccgcccta ctgcgcctgg ccaccgtacc tgctgagcct ccagccggcc tgctgacaga
     4621 gcccctgccg gcgatggagc agcagacggt atttcggctc tctgcacccc gggccacgct
     4681 gcggctgcgc ttccccattg ccgacctgcg gcctgagcgg gacccctggg cgggccaggc
     4741 cgtgcgggct gagcagcttc ggctggagct gagtgagccc cagttccggt cagagcttag
     4801 cagtgggcct ggtcccccag tccccaccca cctggaactc acctgctccg acctacatgg
     4861 tatctatgaa gatggaggga agccacctgt cccttgcctg cgtgtctcca aagccctgga
     4921 ccccaagagc actgggcgca agtacttcct gccccaggta gtggtgactg tgaaccccca
     4981 gtccagcagc acacagtggg aggtggcccc ggagaaggga gaggaactgg agctgtcagt
     5041 ggagagtccc tgtgagctgc gggaacctga gccctcgccc ttctcctcta agaggaccat
     5101 gtatgagaca gaggagatgg tgatccctgg agaccctgag gagatgagga cgttccagag
     5161 ccggaccctg gcactgtccc gctgcagcct ggaagtgatc ctgcccagtg tccacatctt
     5221 tctgcccagc aaggaggtct acgagagcat ctacaacagg atcaacaacg acctgctcat
     5281 gtgggagcct gcagatctgc ttcccacccc cgaccccgcc gcccagccct cgggcttccc
     5341 cggcccctca ggcttctggc acgacagctt taagatgtgc aagtcagcct tcaagctggc
     5401 caactgcttt gatctcaccc cagactcgga ctcggatgac gaggatgccc acttcttctc
     5461 agtgggggca tcaggtggcc cacaggccgc tgcccctgag gccccaagtc ttcacttgca
     5521 gagcaccttc tctacactgg tgacagtgct gaaggggcgg atcacagccc tctgtgagac
     5581 caaggatgag ggtgggaagc ggctggaggc tgtgcacggg gagctggtgc tggacatgga
     5641 gcacggtacc ctcttcagcg tctcccagta ctgtggccag ccaggacttg gctacttctg
     5701 tctggaagct gaaaaggcaa cactctacca ccgagcggcc gtggatgact acccgctgcc
     5761 cagtcacctg gaccttccca gtttcgctcc cccggctcag ctggccccaa ccatctaccc
     5821 atcggaggaa ggggtgaccg agcggggagc ctcgggccgc aagggccagg gccggggacc
     5881 ccacatgttg tccactgctg tgcgcatcca cctggacccc cacaagaatg tgaaggagtt
     5941 cctggtgaca ctgcggttgc acaaagccac cttgcgccac tacatggccc tgcccgagca
     6001 gagctggcat tcccagttgt tggagttcct agacgtgctg gatgaccctg tgctgggcta
     6061 cctgcccccg acggtcatca ccatcctgca cacacacctg ttctcctgct ctgtggacta
     6121 taggccactc tacctcccag tgcgtgtcct catcaccgcg gagaccttca ctctctccag
     6181 caacatcatc atggacacct ccaccttcct gctcaggttc atcctcgatg actccgcctt
     6241 gtacctgtcc gacaagtgtg aggtggagac cctggacctg cggcgagatt atgtctgtgt
     6301 tttggatgtt gacctcttgg aacttgtgat taaaacctgg aaagggagca ccgagggcaa
     6361 actgagccag ccactattcg agctgcgctg ctccaacaat gtggtacacg tgcacagctg
     6421 tgccgactcc tgtgccctgc tggtcaacct gctccagtac gtaatgagca caggcgatct
     6481 gcacccccca ccccggcccc ccagccccac ggagatcgcc ggccagaagc tctcggagag
     6541 tcctgcctct ctgccctcgt gccccccagt ggagacggcc ctcatcaacc agcgtgacct
     6601 ggccgacgcc ctcctggaca ccgagcgcag cctacgggag ctggcccagc cttcaggtgg
     6661 ccacctccct caggcgtcgc ccatctccgt ctacctattc ccaggtgaac ggagtggggc
     6721 cccaccccct tcaccacctg tcgggggccc tgctggcagc ttagggtcat gctcagagga
     6781 gaaggaagat gaaagggaag aggagggcga tggagacacc ctggacagtg atgagttctg
     6841 catccttgat gctcccggcc tgggcatccc gccccgagat ggggagcctg tggtgacaca
     6901 gctgcatccc ggccccatcg ttgtgaggga cggttacttc tcacggccga tcggcagcac
     6961 ggacttgctg cgggcacctg cccatttccc agtgcccagc actcgggtgg tgctacgtga
     7021 ggtctccctc gtctggcacc tctatggggg ccgagacttt ggcccccacc ccggccacag
     7081 ggcaagaact ggcctctcag gtcccaggag ctccccttcc cgctgctctg gccccaaccg
     7141 gccccagaac tcatggcgca cgcagggggg cagcgggcgg cagcaccatg tcctcatgga
     7201 gatccagctg agcaaggtaa gcttccagca cgaggtgtac ccagcggagc cagccacagg
     7261 ccctgcggcc cccagccagg agctggagga gcgaccgctg tcccgtcagg tgttcatcgt
     7321 gcaggagctg gaggtccgag accggctcgc ctcctcccag atcaacaagt tcctgtacct
     7381 acacacgagt gagcggatgc cgcgacgtgc ccactctaac atgctcacca tcaaagcgct
     7441 gcatgtggcc cccactacca acctgggtgg gcctgagtgc tgtctccgcg tctcgctgat
     7501 gcccctgcgg ctcaatgtgg accaggatgc cctcttcttc ctcaaggact tcttcactag
     7561 tctggtggcc ggcatcaacc ccgtggtccc aggggagacc tccgctgagg ctcgccccga
     7621 gactcgagcc cagcccagca gccccctgga agggcaggcc gaaggcgtag agaccactgg
     7681 ttcgcaggag gccccaggag gtggacacag cccctcccct cctgaccagc agcccatcta
     7741 cttcagagag ttccgcttca cgtctgaggt ccccatctgg ctggattacc atggcaagca
     7801 cgtcacgatg gaccaggtgg gcacttttgc tggcctcctc atcggcctgg cccaactcaa
     7861 ctgctccgag ctgaagctaa agcggctctg ttgcaggcac gggctcctgg gtgtggacaa
     7921 ggtgctgggc tatgccctca acgagtggct gcaggacatc cgcaagaacc agctgcccgg
     7981 cctgctggga ggcgtgggcc ccatgcactc ggttgtccag ctcttccaag ggttccggga
     8041 cctgctgtgg ctgcccattg agcagtacag gaaggatggc cgcctcatgc gggggctgca
     8101 gcgaggggct gcctcctttg gctcatccac agcctctgcc gccctggaac tcagcaaccg
     8161 gttggtacag gctatccagg ccacagctga gaccgtgtat gacatcctgt ccccggcagc
     8221 ccccgtctcc cgctccctgc aggataagcg ctctgcgcgg aggctgcgca ggggccagca
     8281 gcctgccgac ctgcgggagg gtgtggccaa ggcctacgac acagtgcgag agggcatctt
     8341 ggatacagct cagaccatct gtgacgtggc atcgcggggc catgagcaga aggggctgac
     8401 gggcgccgtg gggggcgtga tccgccagct gcccccgact gtggtgaagc cgctcatcct
     8461 ggccacggag gccacgtcca gcctgctcgg gggcatgcgc aaccagattg tccccgacgc
     8521 ccacaaggac cacgccctca agtggcgctc ggacagtgcc caagactgag aattcacgcg
     8581 ttcgcgagtt aacggatccc tcgagcggcc gctacgtaaa ttccgccccc ccccctaacg
     8641 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     8701 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     8761 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     8821 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     8881 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     8941 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     9001 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     9061 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     9121 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     9181 gatgataata tggccacaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     9241 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     9301 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     9361 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     9421 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     9481 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     9541 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     9601 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     9661 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     9721 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     9781 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     9841 gcacgacccc atggctcgac gataaaataa aagattttat ttagtctcca gaaaaagggg
     9901 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     9961 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
    10021 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
    10081 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
    10141 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
    10201 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
    10261 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
    10321 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
    10381 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
    10441 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca
    10501 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt
    10561 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10621 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11041 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11221 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11341 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11401 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11461 attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc aatctaaagt
    11521 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11581 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11641 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11701 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11761 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11821 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    11881 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11941 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12001 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12061 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12121 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
    12181 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12241 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12301 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12361 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12421 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12481 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    12541 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    12601 tttcgtcttc actcgaggag cttggcccat tgcatacgtt gtatccatat cataatatgt
    12661 acatttatat tggctcatgt ccaacattac cgccatgtt
//
