LOCUS       sequence-22259-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2T
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(706..730)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caggttcnnn tgaatatttt gtttacttta agaaggagat atacatatgt cgtactacca
       61 tcaccatcac catcacctcg aatcaacaag tttgtacaaa aaagttggga tgcagagagc
      121 ttcacgtctg aagagagagc tgcacatgtt agccacagag ccacccccag gcatcacatg
      181 ttggcaagat aaagaccaaa tggatgacct gcgagctcaa atattaggtg gagccaacac
      241 accttatgag aaaggtgttt ttaagctaga agttatcatt cctgagaggt acccatttga
      301 acctcctcag atccgatttc tcactccaat ttatcatcca aacattgatt ctgctggaag
      361 gatttgtctg gatgttctca aattgccacc aaaaggtgct tggagaccat ccctcaacat
      421 cgcaactgtg ttgacctcta ttcagctgct catgtcagaa cccaaccctg atgacccgct
      481 catggctgac atatcctcag aatttaaata taataagcca gccttcctca agaatgccag
      541 acagtggaca gagaagcatg caagacagaa acaaaaggct gatgaggaag agatgcttga
      601 taatctcaca gnaggtgggn gacngccggg agnacccaac tcaacacaga aaaggaaggc
      661 cagtcagcta gtaggctana aaaaaanttt ttcatcctga tgtttaccca actttcttgt
      721 acaaagtggt tgatncgagn tgctacaagc ccgaaaggan ctgattggtg ctgcnccccg
      781 anaaaaaaac tannancccn tggggctcta acggtntgag ggtttttgt
//
