LOCUS       sequence-222592-        9950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding single copy of the 
            FLAG epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_1XFLAG
FEATURES             Location/Qualifiers
     source          1..9950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3922..3945
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3975..4162
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4030..4054)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4237..5593
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4237..4580
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4581..5390
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4648..4667)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5396..5593
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(5688..5705)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5688..5704)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5697..5719)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8599..9693
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcactag tgactacaag gacgacgatg acaagtctag atgaggcgcg
     3961 ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa
     4021 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat
     4081 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc
     4141 ttattgacca cacctctacc ggcagatccg ctagggataa cagggtaata tagatctgtt
     4201 tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc
     4261 cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag
     4321 cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga
     4381 agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac
     4441 gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca
     4501 ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc
     4561 acatccgaac ataaacaacc atgggtaagg aaaagactca cgtttcgagg ccgcgattaa
     4621 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     4681 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
     4741 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     4801 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     4861 tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa tatcctgatt
     4921 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     4981 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     5041 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     5101 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
     5161 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     5221 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     5281 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     5341 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagtactga
     5401 caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt
     5461 tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg
     5521 cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct
     5581 ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtcgacg aactagtata
     5641 acttcgtata tggtattata tacgaagtta tgcggccgcg cgaattcact ggccgtcgtt
     5701 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5761 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5821 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     5881 ggtatttcac accgcatacc atcgtccgga tcaatgaagt agatgagttt actacctgta
     5941 tatacttttt tttttgtcaa agtcactttt atggcatttc catgttgaag gattagccac
     6001 agcactaaat tttcagcgta tgattgcttt taaatattta attttcatcg ttttttaatt
     6061 aatattcaaa acgatttaat gccttctaca tctgaagtca agctcataga ctcgggttgg
     6121 gtagctaaaa ctacatctaa cacgaagggg tatagccttc ttaaagtata ctcatgtata
     6181 aacgttttgg gttcatattt ttgggccacg tggtttataa aaattcttaa ctacaccact
     6241 cggtatcccg cacccgtcta cgtttctacg atttcgagct aatattgagt aaagtgatta
     6301 gcaaaaaaaa taaaagtact cgttgtcgga gatcaagaat ttttctatta tctcatctaa
     6361 accactttct aaaagcgaaa aacaaaatcg taatatgcag cttgaatggg cttccatagt
     6421 ttgaaagaaa aaccctagca gtactggcaa gggagacatt ccttttacct ggctaatcgc
     6481 catcttccag caggcgcacc attgcccctg tttcactatc caggttacgg atatagttca
     6541 tgacaatatt tacattggtc cagccaccag cttgcatgat ctccggtatt gaaactccag
     6601 cgcgggccat atctcgcgcg gctccgacac gggcactgtg tccagaccag gccaggtatc
     6661 tctgaccaga gtcatcctta gcgccgtaaa tcaatcgatg agttgcttca aaaatccctt
     6721 ccagggcgcg agttgatagc tggctggtgg cagatggcgc ggcaacacca ttttttctga
     6781 cccggcaaaa caggtagtta ttcggatcat cagctacacc agagacggaa atccatcgct
     6841 cgaccagttt agttaccccc aggctaagtg ccttctctac acctgcggtg ctaaccagcg
     6901 ttttcgttct gccaatatgg attaacattc tcccaccgtc agtacgtgag atatctttaa
     6961 ccctgatcct ggcaatttcg gctatacgta acagggtgtt ataagcaatc cccagaaatg
     7021 ccagattacg tatatcctgg cagcgatcgc tattttccat gagtgaacga acctggtcga
     7081 aatcagtgcg ttcgaacgct agagcctgtt ttgcacgttc accggcatca acgttttctt
     7141 ttcggatccg ccgcataacc agtgaaacag cattgctgtc acttggtcgt ggcagcccgg
     7201 accgacgatg aagcatgttt agctggccca aatgttgctg gatagttttt actgccagac
     7261 cgcgcgcctg aagatataga agataatcgc gaacatcttc aggttctgcg ggaaaccatt
     7321 tccggttatt caacttgcac catgccgccc acgaccggca aacggacaga agcattttcc
     7381 aggtatgctc agaaaacgcc tggcgatccc tgaacatgtc catcaggttc ttgcgaacct
     7441 catcactcgt tgcatcgacc ggtaactttt agattaacat ttgtttaaat atgaaattag
     7501 gcaatgtaac atactgcagg caaattttgg tgtacggtaa gtaaattgga catatgattt
     7561 aacaaagcga ctataagtca gaaagtgaga atgagattga aataattaat tcaacttatt
     7621 caattgatga tatgccagga ttcctcttcc tttatcttca atgtttcgat taacaggtcc
     7681 attatccggt ttttagtttc gctgcacatt gccgaatgac aatcggattc ctttaagctt
     7741 tgcttttgcc ctgttttctc tccccagtaa tcgttgggtg actgaaccat ccagtagccg
     7801 agagttacgt ttttcctgat ttaataaata tccatatcta tttgtctcgt ggtgttttac
     7861 agcaactaac cgaaattact attttgcatc atccaaccat aaagagacaa caataattct
     7921 agaaaacata taacaagtgt ttttttctat ttacgtctca tacgcaaagt ccactttacc
     7981 atgttttgga aacttgtacc caaccattca tttttcgatt ttgtaatgag gctcttttct
     8041 tctaacaatc attgaggtca ttatcatgtg tcctcaccta ttgtttaaat tgaggcttgc
     8101 gctattccca aaattgtggg tattattttc atatatcagt atctgcaatt tcggttcaac
     8161 tccggaagct cggtacccaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga
     8221 atctcttagc aaccattatt ttttttctct caacataacg agaacacaca ggggcgctat
     8281 cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc
     8341 atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg
     8401 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt
     8461 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact
     8521 ttctaacttt tcttaccttt tacatttcag caatatatat atatatatat atatatattt
     8581 caaggatata ccattgtaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac
     8641 gttggtcaag aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt
     8701 tccaatgtca agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca
     8761 ggtgttccac ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta
     8821 ggtgctgtgg gtggtcctaa atggggtacc ggtagtgtta gacctgaaca aggtttacta
     8881 aaaatccgta aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac
     8941 tctcttttag acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt
     9001 gttagagaat tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt
     9061 gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc
     9121 gctttcatgg ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat
     9181 gttttggcct cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgaattt
     9241 cctacattga aagttcaaca tcaattgatt gattctgccg ccatgatcct agttaagaac
     9301 ccaacccacc taaatggtat tataatcacc agcaacatgt ttggtgatat catctccgat
     9361 gaagcctccg ttatcccagg ctccttgggt ttgttgccat ctgcgtcctt ggcctctttg
     9421 ccagacaaga acaccgcatt tggtttgtac gaaccatgcc atggttccgc tccagatttg
     9481 ccaaagaata aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg
     9541 tcattgaact tgcctgaaga aggtaaagcc attgaagatg cagttaaaaa ggttttggat
     9601 gcaggtatca gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct
     9661 gtcgccgaag aagttaagaa aatccttgct taaaaagatt ctcttttttt gtgatatttg
     9721 tacataaact ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata
     9781 tgttcatagg gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa
     9841 aaggaggatg taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg
     9901 aaaaagaatt gcactttaac attaatattg acaaggagga gggcatcaca
//
