LOCUS       sequence-222600-       11660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Vo BT, Kwon JA, Li C, Finkelstein D, Xu B, Orr BA, Sherr CJ, Roussel
            MF
  TITLE     Mouse medulloblastoma driven by CRISPR activation of cellular Myc.
  JOURNAL   Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1038/s41598-018-24956-1.
  PUBMED    29880921
REFERENCE   2  (bases 1 to 11660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-7"; volume: "8(1):8733. doi"; pages: " 
            10.1038/s41598-018-24956-1"
COMMENT     Sequence Label: pT2K-CAGGS-U6-stuffer (infusion)-IRES-CFP
FEATURES             Location/Qualifiers
     source          1..11660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2499..2510,2511..2673)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     misc_RNA        complement(3092..3167)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5049..5297)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(5107..5126)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(5277..5297)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        5300..5679
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5681..5956
                     /label=chicken beta-actin promoter
     intron          5957..6977
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6897..6919
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6985..7004
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    7093..7642
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7237..7254)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7464..7483
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7645..7647,7648..7650,7651..8364)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7690..7711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7951..7970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8298..8319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8402..8421)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8467..8522
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8521..8540)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(9190..9377,9378..9389)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9423..9439)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9423..9439)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9476..9496)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9476..9494)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9515..9531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9515..9531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9528..9550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9539..9555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9563..9569,9570..9587,9588..9593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9608..9629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9746..9763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9917..10505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9997..10016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10676..11467,11468..11536))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11299..11318
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11537..11641)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg gggggnatac aacaacacac ttaagtcaca tttgcctacg
     1621 taaagaaaag taaagaaaat caatagctat attttacatc tccttttttt gctgtctttt
     1681 aattagcctt gttttgctgt tatcttgatt gaaactgtaa cttcttcacc tgcttctttt
     1741 ctttgtaggt tttgccagcc cagaaacagg catggctgtg caaggccaga gcaccatgca
     1801 caatgcgctg catgtcttca tgaacggctc aatgtcctca gtccagggct cagccaacga
     1861 ccccatcttc ctccttcacc atgctttcat tgacaggtaa caaacacgtc atgacattag
     1921 actgcacagt ttttgacaaa gttcatacaa tctgttgttt atagctgcta caattagtga
     1981 agtttgtgaa tgtacttgga tgagcagcga aagatcaatt gagatcaatt gttagagttt
     2041 ggttgccctg cagagcaaag aacaaaaaat aatctggtgg ctttactgcg tgaggttatt
     2101 attggtggaa tagaaacaca aaacataatt gcatttattt gtttaatttt ttatcttatc
     2161 ttaactttca tcttgcatat ttgtttctta catcatttct agcatctttg agcgctggct
     2221 aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa
     2281 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
     2341 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
     2401 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
     2461 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     2521 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     2581 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     2641 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     2701 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     2761 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     2821 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     2881 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     2941 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     3001 agagactccc tggtgtctga aacacaggcc agatgggccc tcgtcgacca attcccactc
     3061 ctttcaagac ctagctagcg aattcaaaaa agcaccgact cggtgccact ttttcaagtt
     3121 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacaga gacgtacaaa
     3181 aaagagcaag aagctaaaaa agatttaaaa attattttta gcgcagttaa tggaacagga
     3241 actaaattta ccccaaaaat attacgtgaa tcaggatata acgttattga ggttgaagag
     3301 catgcatttg aagatgaaac atttaaaaat gttgtaaatc caaatccaga atttgatcct
     3361 gcatgaaaaa taccgcttga atatggtatt aaacatgatg cagatattat tattatgaat
     3421 gacccagatg ctgacagatt tggaatggca ataaaacatg atggtcattt tgtaagatta
     3481 gatggaaatc aaacaggacc aattttaatt gattgaaaat tatcaaatct aaaacgctta
     3541 aatagcattc caaaaaatcc ggctctatat tcaagttttg taacaagtga tttgggtgat
     3601 agaatcgctc atgaaaaata tggagttaat attgtaaaaa ctttaactgg atttaaatga
     3661 atgggtagag aaattgctaa agaagaagat aacggattaa attttgtttt tgcttatgaa
     3721 gaaagttatg gatatgtaat tgatgactca gctagagata aagatggaat acaagcttct
     3781 atattaatag cagaggctgc ttgattttat aaaaaacaaa ataaaacatt agtagactat
     3841 ttagaagatt tatttaaaga aatgggtgca tattacactt tcactttaaa cttgaatttt
     3901 aaaccagaag aaaagaaatt aaaaattgaa ccattaatga aatcattgag agcaacaccc
     3961 ttaactcaaa ttgctggact taaagttgtt aatgttgaag actacatcga tggaatgtat
     4021 aatatgccag gacaagactt actaaaattt tatttagaag ataagtcatg atttgctgtt
     4081 cgcccaagtg gaactgaacc taaactaaaa atttatttta taggtgttgg tgaatctgtt
     4141 caaaacgcta aagttaaagt agacgaaatt attaaagaat taaaattaaa aatgaatata
     4201 taggagaaaa aatgaaacta aacaaatata tagatcacac attattaaaa caagatgcta
     4261 cgaaagctga aattaaacaa ttatgtgatg aagcaattga atttgatttt gcaacagttt
     4321 gtgttaattc atattgaaca agctattgta aagaattatt aaaaggcaca aatgtaggaa
     4381 taacaaatgt tgtaggtttt cctctaggtg catgcacaac agctacaaaa gcattcgaag
     4441 tttctgaagc aattaaagat ggtgcaacag aaattgatat ggtattaaat attggtgcat
     4501 taaaagacaa aaattatgaa ttagttttag aagacatgaa agctgtaaaa aaagcagctg
     4561 gatcacatgt tgttaaatgt attatggaaa attgtttatt aacaaaagaa gaaatcatga
     4621 aagcttgtga aatagctgtt gaagctggat tagaatttgt taaaacatca acaggatttt
     4681 caaaatcagg tgcaacattt gaagatgtta aactaatgaa gtcagttgtt aaagacaatg
     4741 ctttagttaa agcagctggt ggagttagaa catttgaaga tgctcaaaaa atgattgaag
     4801 caggagctga ccgcttagga acaagtggtg gagtagctat tattaaaggt gaagaaaaca
     4861 acgcgagtta ctaaaactag cgttttttta ttttgctcat ttttattaaa agtttgcaaa
     4921 aaggaacata aaaattctaa ttattgatac taaagttatt aaaaagaaga ttttggttga
     4981 ttttataaag gtcatagaat ataatatttt agcatgtgta ttttgtgtgc tcatttacaa
     5041 ccgtctccgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
     5101 caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
     5161 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
     5221 aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc
     5281 atgggaaata ggccctctcg acattgatta ttgactagtt attaatagta atcaattacg
     5341 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     5401 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     5461 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     5521 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     5581 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     5641 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     5701 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     5761 taattatttt gtgcagcgat gggggcgggg gggggggggg sgcgcgccrg gsggggsggg
     5821 gsggggcgrg gggsggggsg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     5881 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     5941 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     6001 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     6061 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     6121 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     6181 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     6241 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     6301 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     6361 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     6421 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     6481 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     6541 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     6601 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     6661 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     6721 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     6781 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     6841 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     6901 cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct
     6961 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     7021 gcaaagaatt cagatcttac gtagctagcg gatcccaatt gctcgagcgg gatcaattcc
     7081 gccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     7141 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     7201 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     7261 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     7321 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     7381 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     7441 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     7501 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     7561 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     7621 tcctttgaaa aacacgataa taccatggtg agcaagggcg aggagctgtt caccggggtg
     7681 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     7741 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     7801 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
     7861 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     7921 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     7981 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     8041 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
     8101 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     8161 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     8221 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     8281 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     8341 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gagtcgagtc tagatatcag
     8401 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     8461 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     8521 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     8581 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     8641 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     8701 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     8761 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     8821 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ctgcgaagat
     8881 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     8941 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     9001 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     9061 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     9121 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     9181 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     9241 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     9301 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     9361 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     9421 cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     9481 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9541 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9601 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9661 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9721 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9781 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9841 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9901 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9961 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10021 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10081 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10141 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10201 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10261 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10321 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10381 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10501 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10561 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10621 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10681 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10741 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10801 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10861 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10921 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10981 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11041 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11101 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11161 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11221 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11281 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11341 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11401 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11461 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11521 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11581 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11641 gcacatttcc ccgaaaagtg
//
