LOCUS       sequence-222605-        9800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA targeting a sequence upstream of the initiator ATG of the 
            cellular Myc gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9800)
  AUTHORS   Vo BT, Kwon JA, Li C, Finkelstein D, Xu B, Orr BA, Sherr CJ, Roussel
            MF
  TITLE     Mouse medulloblastoma driven by CRISPR activation of cellular Myc.
  JOURNAL   Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1038/s41598-018-24956-1.
  PUBMED    29880921
REFERENCE   2  (bases 1 to 9800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-7"; volume: "8(1):8733. doi"; pages: " 
            10.1038/s41598-018-24956-1"
COMMENT     Sequence Label: pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP
FEATURES             Location/Qualifiers
     source          1..9800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2499..2510,2511..2673)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     misc_RNA        complement(3092..3167)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3188..3436)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3246..3265)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3416..3436)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        3439..3818
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3820..4096
                     /label=chicken beta-actin promoter
     intron          4097..5117
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5037..5059
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5125..5144
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    5233..5782
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5377..5394)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5604..5623
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5785..5787,5788..5790,5791..6504)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5830..5851)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6091..6110)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6438..6459
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6542..6561)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6607..6662
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6661..6680)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(7330..7517,7518..7529)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7563..7579)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7563..7579)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7616..7636)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7616..7634)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7655..7671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7655..7671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7668..7690)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7679..7695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7703..7709,7710..7727,7728..7733))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7748..7769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7886..7903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8057..8645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8137..8156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8816..9607,9608..9676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9439..9458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9677..9781)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg gggggnatac aacaacacac ttaagtcaca tttgcctacg
     1621 taaagaaaag taaagaaaat caatagctat attttacatc tccttttttt gctgtctttt
     1681 aattagcctt gttttgctgt tatcttgatt gaaactgtaa cttcttcacc tgcttctttt
     1741 ctttgtaggt tttgccagcc cagaaacagg catggctgtg caaggccaga gcaccatgca
     1801 caatgcgctg catgtcttca tgaacggctc aatgtcctca gtccagggct cagccaacga
     1861 ccccatcttc ctccttcacc atgctttcat tgacaggtaa caaacacgtc atgacattag
     1921 actgcacagt ttttgacaaa gttcatacaa tctgttgttt atagctgcta caattagtga
     1981 agtttgtgaa tgtacttgga tgagcagcga aagatcaatt gagatcaatt gttagagttt
     2041 ggttgccctg cagagcaaag aacaaaaaat aatctggtgg ctttactgcg tgaggttatt
     2101 attggtggaa tagaaacaca aaacataatt gcatttattt gtttaatttt ttatcttatc
     2161 ttaactttca tcttgcatat ttgtttctta catcatttct agcatctttg agcgctggct
     2221 aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa
     2281 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
     2341 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
     2401 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
     2461 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     2521 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     2581 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     2641 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     2701 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     2761 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     2821 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     2881 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     2941 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     3001 agagactccc tggtgtctga aacacaggcc agatgggccc tcgtcgacca attcccactc
     3061 ctttcaagac ctagctagcg aattcaaaaa agcaccgact cggtgccact ttttcaagtt
     3121 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacagc gtcggcgaag
     3181 ccctgccggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc
     3241 aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc
     3301 caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca
     3361 aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca
     3421 tgggaaatag gccctctcga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3481 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3541 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3601 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3661 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3721 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3781 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     3841 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     3901 aattattttg tgcagcgatg ggggcggggg gggggggggn sgcgcgccrg gsggggsggg
     3961 gsggggsgrg gggsggggsg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     4021 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     4081 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     4141 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     4201 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     4261 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     4321 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     4381 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     4441 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     4501 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     4561 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     4621 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     4681 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     4741 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     4801 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     4861 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     4921 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     4981 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     5041 cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct
     5101 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     5161 gcaaagaatt cagatcttac gtagctagcg gatcccaatt gctcgagcgg gatcaattcc
     5221 gccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5281 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5341 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5401 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5461 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5521 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5581 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     5641 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     5701 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5761 tcctttgaaa aacacgataa taccatggtg agcaagggcg aggagctgtt caccggggtg
     5821 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     5881 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     5941 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
     6001 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6061 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6121 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6181 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
     6241 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     6301 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     6361 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     6421 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     6481 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gagtcgagtc tagatatcag
     6541 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     6601 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     6661 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     6721 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     6781 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     6841 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     6901 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     6961 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ctgcgaagat
     7021 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     7081 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     7141 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     7201 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     7261 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     7321 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     7381 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     7441 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     7501 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     7561 cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     7621 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7681 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7741 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7801 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7861 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7921 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7981 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8041 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8101 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8161 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8221 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8281 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8341 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8401 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8461 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8521 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8581 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8641 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8701 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8761 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8821 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8881 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8941 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9001 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9061 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9121 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9781 gcacatttcc ccgaaaagtg
//
