LOCUS       sequence-222610-       10584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3 sgRNAs targeting a sequence upstream of the initiator ATG of the 
            cellular Myc gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Vo BT, Kwon JA, Li C, Finkelstein D, Xu B, Orr BA, Sherr CJ, Roussel
            MF
  TITLE     Mouse medulloblastoma driven by CRISPR activation of cellular Myc.
  JOURNAL   Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1038/s41598-018-24956-1.
  PUBMED    29880921
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-7"; volume: "8(1):8733. doi"; pages: " 
            10.1038/s41598-018-24956-1"
COMMENT     Sequence Label: 
            pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP
FEATURES             Location/Qualifiers
     source          1..10584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2499..2510,2511..2673)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     misc_RNA        complement(3092..3167)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3189..3437)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(3482..3557)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3579..3827)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(3876..3951)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3972..4220)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     enhancer        4223..4602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4604..4880
                     /label=chicken beta-actin promoter
     intron          4881..5901
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5821..5843
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5909..5928
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    6017..6566
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6161..6178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6388..6407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6569..6571,6572..6574,6575..7288)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6614..6635)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6875..6894)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7222..7243
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7326..7345)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7391..7446
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7445..7464)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(8114..8301,8302..8313)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8347..8363)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8347..8363)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8400..8420)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8400..8418)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8439..8455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8439..8455)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8452..8474)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8463..8479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8487..8493,8494..8511,8512..8517))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8532..8553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8670..8687)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8841..9429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8921..8940)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9600..10391,10392..10460))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10223..10242
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10461..10565)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg gggggnatac aacaacacac ttaagtcaca tttgcctacg
     1621 taaagaaaag taaagaaaat caatagctat attttacatc tccttttttt gctgtctttt
     1681 aattagcctt gttttgctgt tatcttgatt gaaactgtaa cttcttcacc tgcttctttt
     1741 ctttgtaggt tttgccagcc cagaaacagg catggctgtg caaggccaga gcaccatgca
     1801 caatgcgctg catgtcttca tgaacggctc aatgtcctca gtccagggct cagccaacga
     1861 ccccatcttc ctccttcacc atgctttcat tgacaggtaa caaacacgtc atgacattag
     1921 actgcacagt ttttgacaaa gttcatacaa tctgttgttt atagctgcta caattagtga
     1981 agtttgtgaa tgtacttgga tgagcagcga aagatcaatt gagatcaatt gttagagttt
     2041 ggttgccctg cagagcaaag aacaaaaaat aatctggtgg ctttactgcg tgaggttatt
     2101 attggtggaa tagaaacaca aaacataatt gcatttattt gtttaatttt ttatcttatc
     2161 ttaactttca tcttgcatat ttgtttctta catcatttct agcatctttg agcgctggct
     2221 aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa
     2281 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
     2341 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
     2401 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
     2461 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     2521 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     2581 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     2641 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     2701 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     2761 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     2821 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     2881 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     2941 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     3001 agagactccc tggtgtctga aacacaggcc agatgggccc tcgtcgacca attcccactc
     3061 ctttcaagac ctagctagcg aattcaaaaa agcaccgact cggtgccact ttttcaagtt
     3121 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacttc cccacctctc
     3181 tctatttcgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
     3241 caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
     3301 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
     3361 aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc
     3421 atgggaaata ggccctccca attcccactc ctttcaagac ctagctagcg aattcaaaaa
     3481 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
     3541 atttctagct ctaaaacgta ctccggctcc ggggtgtcgg tgtttcgtcc tttccacaag
     3601 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
     3661 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
     3721 ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc
     3781 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctctcg accaattccc
     3841 actcctttca agacctagct agcgaattca aaaaagcacc gactcggtgc cactttttca
     3901 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa cagcgtcggc
     3961 gaagccctgc cggtgtttcg tcctttccac aagatatata aagccaagaa atcgaaatac
     4021 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac
     4081 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca
     4141 gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga
     4201 atcatgggaa ataggccctc tcgacattga ttattgacta gttattaata gtaatcaatt
     4261 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4321 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4381 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     4441 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     4501 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     4561 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     4621 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     4681 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggnsgcgcg ccrggsgggg
     4741 sggggsgggg sgrggggsgg ggsggggcga ggcggagagg tgcggcggca gccaatcaga
     4801 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     4861 agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg
     4921 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     4981 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     5041 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     5101 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     5161 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     5221 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     5281 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     5341 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     5401 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     5461 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     5521 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     5581 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     5641 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     5701 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     5761 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     5821 ggttcggctt ctggcgtgtg accggcggct ctagctagag cctctgctaa ccatgttcat
     5881 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     5941 tttggcaaag aattcagatc ttacgtagct agcggatccc aattgctcga gcgggatcaa
     6001 ttccgccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6061 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     6361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6541 gttttccttt gaaaaacacg ataataccat ggtgagcaag ggcgaggagc tgttcaccgg
     6601 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     6661 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     6721 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
     6781 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     6841 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     6901 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     6961 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt
     7021 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     7081 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     7141 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     7201 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     7261 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagagtcg agtctagata
     7321 tcagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     7381 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     7441 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     7501 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     7561 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     7621 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     7681 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     7741 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttctgcga
     7801 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
     7861 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
     7921 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
     7981 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
     8041 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
     8101 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
     8161 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
     8221 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
     8281 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
     8341 ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
     8401 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     8461 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8521 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8581 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8641 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8701 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8761 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8821 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8881 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8941 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9001 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9061 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9121 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9181 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9241 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9301 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9361 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9421 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9481 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9541 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9601 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9661 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9721 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9781 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9841 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9901 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9961 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10021 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10081 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10141 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10201 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10261 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10321 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10381 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10441 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10501 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10561 ccgcgcacat ttccccgaaa agtg
//
