LOCUS       sequence-222631-        9801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA targeting a sequence upstream of the initiator ATG of the 
            cellular Myc gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Vo BT, Kwon JA, Li C, Finkelstein D, Xu B, Orr BA, Sherr CJ, Roussel
            MF
  TITLE     Mouse medulloblastoma driven by CRISPR activation of cellular Myc.
  JOURNAL   Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1038/s41598-018-24956-1.
  PUBMED    29880921
REFERENCE   2  (bases 1 to 9801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-7"; volume: "8(1):8733. doi"; pages: " 
            10.1038/s41598-018-24956-1"
COMMENT     Sequence Label: pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP
FEATURES             Location/Qualifiers
     source          1..9801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2499..2510,2511..2673)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     misc_RNA        complement(3092..3167)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3189..3437)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3247..3266)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3417..3437)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        3440..3819
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4098..5118
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5038..5060
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5126..5145
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    5234..5783
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5378..5395)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5605..5624
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5786..5788,5789..5791,5792..6505)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5831..5852)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6092..6111)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6439..6460
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6543..6562)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6608..6663
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6662..6681)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(7331..7518,7519..7530)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7564..7580)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7564..7580)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7617..7637)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7617..7635)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7656..7672)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7656..7672)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7669..7691)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7680..7696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7704..7710,7711..7728,7729..7734))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7749..7770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7887..7904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8058..8646)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8138..8157)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8817..9608,9609..9677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9440..9459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9678..9782)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg gggggnatac aacaacacac ttaagtcaca tttgcctacg
     1621 taaagaaaag taaagaaaat caatagctat attttacatc tccttttttt gctgtctttt
     1681 aattagcctt gttttgctgt tatcttgatt gaaactgtaa cttcttcacc tgcttctttt
     1741 ctttgtaggt tttgccagcc cagaaacagg catggctgtg caaggccaga gcaccatgca
     1801 caatgcgctg catgtcttca tgaacggctc aatgtcctca gtccagggct cagccaacga
     1861 ccccatcttc ctccttcacc atgctttcat tgacaggtaa caaacacgtc atgacattag
     1921 actgcacagt ttttgacaaa gttcatacaa tctgttgttt atagctgcta caattagtga
     1981 agtttgtgaa tgtacttgga tgagcagcga aagatcaatt gagatcaatt gttagagttt
     2041 ggttgccctg cagagcaaag aacaaaaaat aatctggtgg ctttactgcg tgaggttatt
     2101 attggtggaa tagaaacaca aaacataatt gcatttattt gtttaatttt ttatcttatc
     2161 ttaactttca tcttgcatat ttgtttctta catcatttct agcatctttg agcgctggct
     2221 aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa
     2281 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
     2341 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
     2401 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
     2461 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     2521 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     2581 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     2641 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     2701 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     2761 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     2821 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     2881 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     2941 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     3001 agagactccc tggtgtctga aacacaggcc agatgggccc tcgtcgacca attcccactc
     3061 ctttcaagac ctagctagcg aattcaaaaa agcaccgact cggtgccact ttttcaagtt
     3121 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacttc cccacctctc
     3181 tctatttcgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
     3241 caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
     3301 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
     3361 aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc
     3421 atgggaaata ggccctctcg acattgatta ttgactagtt attaatagta atcaattacg
     3481 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     3541 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3601 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3661 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     3721 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     3781 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     3841 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     3901 taattatttt gtgcagcgat gggggcgggg gggggggggg nsgcgcgccr ggsggggsgg
     3961 ggsggggsgr ggggsggggs ggggsgrggc ggrgaggkgc ggcggcagcc aatcagagcg
     4021 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     4081 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     4141 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     4201 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     4261 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     4321 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     4381 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     4441 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     4501 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     4561 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     4621 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     4681 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     4741 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     4801 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     4861 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     4921 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     4981 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     5041 tcggcttctg gcgtgtgacc ggcggctcta gctagagcct ctgctaacca tgttcatgcc
     5101 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     5161 ggcaaagaat tcagatctta cgtagctagc ggatcccaat tgctcgagcg ggatcaattc
     5221 cgcccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5281 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5341 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5401 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5461 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5521 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5581 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5641 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5701 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5761 ttcctttgaa aaacacgata ataccatggt gagcaagggc gaggagctgt tcaccggggt
     5821 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5881 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5941 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
     6001 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6061 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     6121 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     6181 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta
     6241 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     6301 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     6361 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     6421 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6481 cggcatggac gagctgtaca agtaaagcgg ccgcgactct agagtcgagt ctagatatca
     6541 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     6601 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     6661 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     6721 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     6781 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     6841 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     6901 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     6961 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tctgcgaaga
     7021 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
     7081 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
     7141 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
     7201 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
     7261 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
     7321 aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag
     7381 gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt
     7441 tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa
     7501 gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca
     7561 gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     7621 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7681 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7741 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7801 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7861 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7921 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7981 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8041 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8101 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8161 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8221 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8281 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8341 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8401 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8461 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8521 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8581 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8641 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8701 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8761 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8821 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8881 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8941 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9001 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9061 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9121 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9181 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9241 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9301 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9361 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9421 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9481 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9541 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9601 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9661 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9721 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9781 cgcacatttc cccgaaaagt g
//
