LOCUS       sequence-222652-        9441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Pin3Cas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pC0070	Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI
FEATURES             Location/Qualifiers
     source          1..9441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..44
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     128..147
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        128..146
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    147..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             186..208
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             227..244
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             254..271
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(284..307,308..343,344..367)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             368..661
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(4069..4087)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4083..4130
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4305..4310,4311..4327,4328..4333))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4360..4378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4446..4550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4551..4619,4620..5411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4769..4788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5582..6170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6071..6090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6324..6341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6356..6495
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6421..6440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6581..6603
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6597..6788)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8100..9182)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9144..9163
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9183..9260)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
       61 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
      121 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      181 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
      241 tcacagcagc ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt
      301 tgaaaaaggt ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt
      361 tgagaaaatg tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt
      421 caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa
      481 gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa
      541 ggaaatggac tccttaagat tcttgtacga cggtattaga atccaagctg atcagacccc
      601 tgaagatttg gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg
      661 atctatgacc gagcagaacg agaagcccta caacggcacc tactacaccc tggaagataa
      721 gcacttctgg gccgcctttc tgaacctggc cagacacaac gcctacatca cactggccca
      781 catcgacaga cagctggcct acagcaaggc cgacatcacc aacgacgagg acatcctgtt
      841 cttcaaaggc cagtggaaga acctggacaa cgacctggaa cggaaggcca gactgagaag
      901 cctgatcctg aagcacttca gctttctgga aggcgccgct tacggcaaga agctgtttga
      961 gtctcagagc agcggcaaca agagcagcaa gaagaaagag ctgtccaaga aagagaaaga
     1021 ggaactgcag gccaacgctc tgagcctgga caatctgaag tctatcctgt tcgacttcct
     1081 gcagaagctg aaggacttcc ggaactacta cagccactac agacaccccg agagcagcga
     1141 gctgcctctg ttcgatggca acatgctgca gcggctgtac aacgtgttcg acgtgtccgt
     1201 gcagcgcgtg aagagagatc acgagcacaa cgacaaggtg gaccctcacc ggcacttcaa
     1261 tcacctcgtg cggaagggca agaaggataa gtacggcaac aacgataacc cgttcttcaa
     1321 gcaccacttc gtggaccgcg agggcacagt gacagaagcc ggactgctgt tcttcgtgtc
     1381 cctgttcctg gaaaagcggg acgccatctg gatgcagaag aagatccggg gctttaaagg
     1441 cggcaccgag gcctaccagc agatgaccaa tgaggtgttc tgccgcagcc ggatcagcct
     1501 gcctaagctg aagctggaaa gcctgagaac cgacgactgg atgctgctgg acatgctgaa
     1561 cgaactcgtg cggtgccaca agagcctgta cgatagactg agagaagagg accgggccag
     1621 attcagagtg cccgtggata tcctgagcga cgaggatgat accgacggca ccgaagagga
     1681 tcccttcaag aacactctcg tgcggcacca ggacagattc ccttacttcg ccctgcggta
     1741 cttcgacctg aagaaggtgt tcaccagcct gcggttccac atcgatctgg gcacctacca
     1801 cttcgccatc tacaagaaga acatcggcga gcagcccgag gacagacacc tgaccagaaa
     1861 cctgtacggc ttcggccgga tccaggactt cgccgaagaa cacagacccg aggaatggaa
     1921 gcggctcgtc agagatctgg actacttcga gacaggcgac aagccttaca tcacccagac
     1981 cacacctcac taccacatcg agaagggaaa gatcggcctg agattcgtgc ccgagggaca
     2041 gcatctttgg ccctctccag aagtgggcgc caccagaaca ggcagatcta agtacgccca
     2101 ggacaagaga ctgaccgccg aggcctttct gtctgtgcac gagctgatgc ctatgatgtt
     2161 ctactacttc ctgctgcgcg agaagtacag cgaggaagtg tctgccgaga aggtgcaggg
     2221 cagaatcaag cgcgtgatcg aggatgtgta cgccgtgtac gatgccttcg ccagggacga
     2281 gatcaacacc agagatgagc tggatgcctg cctggccgac aagggcatta gaagaggcca
     2341 tctgccaaga cagatgatcg ccatcctgag ccaagagcac aaggacatgg aagagaaagt
     2401 gcggaagaaa ctgcaagaga tgattgccga caccgaccac cggctggata tgctggatag
     2461 acagaccgac cggaagatca gaatcggccg gaaaaatgcc ggcctgccaa agtctggcgt
     2521 ggtggctgat tggctcgtgc gggacatgat gagattccag cctgtggcca aggacaccag
     2581 cggcaagcct ctgaacaact ccaaggccaa cagcaccgag taccggatgc tgcagagagc
     2641 cctggctctg tttggaggcg agaaagagag gctgacccca tacttccggc agatgaatct
     2701 gaccggcgga aacaaccctc atccttttct gcacgaaact cgctgggaga gccacaccaa
     2761 tatcctgtcc ttctaccggt cctacctgga agcccggaag gctttcctgc agtccatcgg
     2821 cagatccgac agagtggaaa accaccggtt tctgctgctg aaagagccca agaccgacag
     2881 gcagactctg gtggctggat ggaagggcga gttccatctg cctagaggca tctttacaga
     2941 ggccgtgcgc gactgcctga tcgagatggg ctatgatgaa gtgggcagct acaaagaagt
     3001 gggattcatg gccaaggccg tgcctctgta ctttgagaga gccagcaagg accgggtgca
     3061 gcccttctac gactacccct tcaacgtggg caacagcctg aagcctaaga agggcagatt
     3121 cctgtccaaa gagaagcggg ccgaagagtg ggagagcggc aaagagagat tccggctggc
     3181 caagctgaag aaagaaatcc tcgaagccaa agagcacccc taccacgact tcaagagctg
     3241 gcagaagttc gagcgcgagc tgagactggt caagaaccag gacatcatca cctggatgat
     3301 gtgccgggac ctgatggaag aaaacaaagt ggaaggcctg gatacaggca ccctgtacct
     3361 gaaggacatc aggaccgacg tgcaagagca gggcagcctg aacgtgctga acagagtgaa
     3421 gcccatgaga ctgcccgtgg tggtgtacag agccgatagc agaggccacg tgcacaaaga
     3481 acaggcccct ctggccaccg tgtacatcga ggaaagagac accaagctgc tgaagcaggg
     3541 caacttcaag tccttcgtga aggacagacg gctgaatggc ctgttcagct tcgtggatac
     3601 tggcgccctg gccatggaac agtaccccat ctctaaactg cgcgtggaat acgagctggc
     3661 caaataccag accgccagag tgtgcgcctt cgagcagaca ctggaactgg aagagagcct
     3721 gctgacccgg tatcctcatc tgcccgacaa gaactttcgg aagatgctcg aatcttggag
     3781 cgaccctctg ctggataagt ggcctgacct gcacggaaat gtgcggctgc tgatcgccgt
     3841 gcggaatgcc tttagccaca atcagtaccc tatgtacgac gagacactgt ttagctccat
     3901 ccggaagtac gaccctagca gccctgacgc cattgaggaa cggatgggcc tgaatatcgc
     3961 ccacagactg tctgaggaag tgaagcaggc caaagaaatg gtggaacgga tcattcaggc
     4021 ctaaggccgc actcgaggcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     4081 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     4141 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
     4201 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
     4261 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat
     4321 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     4381 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4441 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4501 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4561 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4621 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4681 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4741 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     4801 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4861 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4921 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4981 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5041 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     5101 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5161 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5221 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtca cgcggtatca
     5281 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5341 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5401 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5461 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5521 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5581 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5641 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5701 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5761 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5821 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5881 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5941 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6001 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6061 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6121 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6181 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6241 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6301 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     6361 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     6421 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     6481 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     6541 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     6601 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     6661 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     6721 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     6781 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     6841 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     6901 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     6961 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     7021 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     7081 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     7141 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     7201 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
     7261 gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
     7321 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
     7381 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
     7441 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta
     7501 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca
     7561 gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct
     7621 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
     7681 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
     7741 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
     7801 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
     7861 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     7921 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     7981 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     8041 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct
     8101 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8161 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
     8221 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
     8281 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
     8341 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc
     8401 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
     8461 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
     8521 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
     8581 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
     8641 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag
     8701 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
     8761 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
     8821 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
     8881 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
     8941 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
     9001 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
     9061 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
     9121 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
     9181 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
     9241 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag
     9301 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
     9361 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     9421 acaagcgctc atgagcccga a
//
