LOCUS       sequence-222730-        8280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Yan M, Kanbe E, Peterson LF, Boyapati A, Miao Y, Wang Y, Chen IM, 
            Chen Z, Rowley JD, Willman CL, Zhang DE
  TITLE     A previously unidentified alternatively spliced isoform of t(8;21) 
            transcript promotes leukemogenesis.
  JOURNAL   Nat Med. 2006 Aug . 12(8):945-9.
  PUBMED    16892037
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2006 Aug . 12(8):945-9."
COMMENT     Sequence Label: MigR1-AE9a
FEATURES             Location/Qualifiers
     source          1..8280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             32..548
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    612..953
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1020..1436
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1366..1388
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1404..1420
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1471..1497
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3304..3871
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3321..3873
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3465..3482)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3692..3711
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3885..3887,3888..3890,3891..4604)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3930..3951)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4191..4210)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4538..4559
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4706..5220
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5382..5398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5406..5412,5413..5430,5431..5436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5451..5472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5589..5606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5760..6348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5840..5859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6519..7310,7311..7379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7142..7161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7380..7484)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7552..7570
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7608..7630)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7730..7749
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7943..7965
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8093..8112
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
       61 agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
      121 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
      181 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
      241 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
      301 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
      361 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata
      421 gactgcgtcg cccgggtacc cgtattccca ataaagcctc ttgctgtttg catccgaatc
      481 gtggactcgc tgatccttgg gagggtctcc tcagattgat tgactgccca cctcgggggt
      541 ctttcatttg gaggttccac cgagatttgg agacccctgc ccagggacca ccgacccccc
      601 cgccgggagg taagctggcc agcggtcgtt tcgtgtctgt ctctgtcttt gtgcgtgttt
      661 gtgccggcat ctaatgtttg cgcctgcgtc tgtactagtt agctaactag ctctgtatct
      721 ggcggacccg tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc
      781 ccagggactt tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc
      841 cgaccccgtc aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc
      901 cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct
      961 gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg
     1021 gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga
     1081 tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag
     1141 aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca
     1201 cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct
     1261 acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac
     1321 accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc
     1381 gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggaa
     1441 ttagatctct cgaggttcta gaccatggca tacccatacg acgtgcctga ctacgcctcc
     1501 cgtatccccg tagatgccag cacgagccgc cgcttcacgc cgccttccac cgcgctgagc
     1561 ccaggcaaga tgagcgaggc gttgccgctg ggcgccccgg acgccggcgc tgccctggcc
     1621 ggcaagctga ggagcggcga ccgcagcatg gtggaggtgc tggccgacca cccgggcgag
     1681 ctggtgcgca ccgacagccc caacttcctc tgctccgtgc tgcctacgca ctggcgctgc
     1741 aacaagaccc tgcccatcgc tttcaaggtg gtggccctag gggatgttcc agatggcact
     1801 ctggtcactg tgatggctgg caatgatgaa aactactcgg ctgagctgag aaatgctacc
     1861 gcagccatga agaaccaggt tgcaagattt aatgacctca ggtttgtcgg tcgaagtgga
     1921 agagggaaaa gcttcactct gaccatcact gtcttcacaa acccaccgca agtcgccacc
     1981 taccacagag ccatcaaaat cacagtggat gggccccgag aacctcgaaa tcgtactgag
     2041 aagcactcca caatgccaga ctcacctgtg gatgtgaaga cgcaatctag gctgactcct
     2101 ccaacaatgc cacctccccc aactactcaa ggagctccaa gaaccagttc atttacaccg
     2161 acaacgttaa ctaatggcac gagccattct cctacagcct tgaatggcgc cccctcacca
     2221 cccaatggct tcagcaatgg gccttcctct tcttcctcct cctctctggc taatcaacag
     2281 ctgcccccag cctgtggtgc caggcaactc agcaagctga aaaggttcct tactaccctg
     2341 cagcagtttg gcaatgacat ttcacccgag ataggagaaa gagttcgcac cctcgttctg
     2401 ggactagtga actccacttt gacaattgaa gaatttcatt ccaaactgca agaagctact
     2461 aacttcccac tgagaccttt tgtcatccca tttttgaagg ccaacttgcc cctgctgcag
     2521 cgtgagctcc tccactgcgc aagactggcc aaacagaacc ctgcccagta cctcgcccag
     2581 catgaacagc tgcttctgga tgccagcacc acctcacctg ttgactcctc agagctgctt
     2641 ctcgatgtga acgaaaacgg gaagaggcga actccagaca gaaccaaaga aaatggcttt
     2701 gacagagagc ctttgcactc agaacatcca agcaagcgac catgcactat tagcccaggc
     2761 cagcggtaca gtccaaataa cggcttatcc taccagccca atggcctgcc tcaccctacc
     2821 ccacctccac ctcagcatta ccgtttggat gatatggcca ttgcccacca ctacagggac
     2881 tcctatcgac accccagcca cagggacctc agggacagaa acagacctat ggggttgcat
     2941 ggcacacgtc aagaagaaat gattgatcac agactaacag acagagaatg ggcagaagag
     3001 tggaaacatc ttgaccatct gttaaactgc ataatggaca tggtagaaaa aacaaggcga
     3061 tctctcaccg tactaaggcg gtgtcaagaa gcagaccggg aagaattgaa ttactggatc
     3121 cggcggtaca gtgacgccga ggacttaaaa aaaggtggcg gcagtagcag cagccactct
     3181 aggcagcaga gtcccgtcaa cccagaccca gttgcactag gatgaaggcc atacctaggt
     3241 gctgcccagc tttgagcatt gctgtcctgg gtcatatgag gatcgatgga atcactagtg
     3301 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     3361 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3421 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3481 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3541 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3601 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3661 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3721 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3781 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     3841 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg
     3901 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3961 acaagttcag cgtgtctggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     4021 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     4081 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     4141 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     4201 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4261 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4321 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact
     4381 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4441 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     4501 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4561 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatgaatt aattaagaat
     4621 tatcaagctt atcgataccg tcgacctgca gccaagctta tcgataaaat aaaagatttt
     4681 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     4741 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
     4801 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
     4861 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc
     4921 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     4981 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     5041 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg
     5101 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     5161 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
     5221 gggtaacagt ttcttgaagt tggagaacaa cattctgagg gtaggagtcg aatattaagt
     5281 aatcctgact caattagcca ctgttttgaa tccacatact ccaatactcc tgaaatagtt
     5341 cattatggac agcgcagaaa gagctgggga gaattgtgaa attgttatcc gctcacaatt
     5401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7381 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7441 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7501 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7561 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7621 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7681 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7741 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7801 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     7861 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     7921 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc gcaaggaatg
     7981 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     8041 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     8101 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     8161 cggcgtagag gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag
     8221 ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa
//
