LOCUS       sequence-222737-        7326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Yano H, Cong F, Birge RB, Goff SP, Chao MV
  TITLE     Association of the Abl tyrosine kinase with the Trk nerve growth 
            factor receptor.
  JOURNAL   J Neurosci Res. 2000 Feb 1. 59(3):356-64.
  PUBMED    10679771
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Res. 2000 Feb 1. 59(3):356-64."
COMMENT     Sequence Label: pCMV5 TrkA
FEATURES             Location/Qualifiers
     source          1..7326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     576..596
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3391..4013
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3590..3609)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4037..4057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4042..4371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4222..4357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4409..4428)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4410..4428)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4442..4458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4442..4458)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4455..4477)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4466..4482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4490..4496,4497..4514,4515..4520))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4535..4556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4673..4690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4844..5432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4924..4943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5603..6394,6395..6463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6226..6245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6464..6568)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6595..7050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6682..6701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6892..6913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7176..7198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7190..7207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7191..7207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagaattccg cgcacatgtc
      661 gggggaggcc tggcagctgc agctgggagc gcacagacgg ctgccccgcc tgagcgaggc
      721 gggcgccgcc gcgatgctgc gaggcggacg gcgcgggcag cttggctggc acagctgggc
      781 tgcggggccg ggcagcctgc tggcttggct gatactggca tctgcgggcg ccgcaccctg
      841 ccccgatgcc tgctgccccc acggctcctc gggactgcga tgcacccggg atggggccct
      901 ggatagcctc caccacctgc ccggcgcaga gaacctgact gagctctaca tcgagaacca
      961 gcagcatctg cagcatctgg agctccgtga tctgaggggc ctgggggagc tgagaaacct
     1021 caccatcgtg aagagtggtc tccgtttcgt ggcgccagat gccttccatt tcactcctcg
     1081 gctcagtcgc ctgaatctct ccttcaacgc tctggagtct ctctcctgga aaactgtgca
     1141 gggcctctcc ttacaggaac tggtcctgtc ggggaaccct ctgcactgtt cttgtgccct
     1201 gcgctggcta cagcgctggg aggaggaggg actgggcgga gtgcctgaac agaagctgca
     1261 gtgtcatggg caagggcccc tggcccacat gcccaatgcc agctgtggtg tgcccacgct
     1321 gaaggtccag gtgcccaatg cctcggtgga tgtgggggac gacgtgctgc tgcggtgcca
     1381 ggtggagggg cggggcctgg agcaggccgg ctggatcctc acagagctgg agcagtcagc
     1441 cacggtgatg aaatctgggg gtctgccatc cctggggctg accctggcca atgtcaccag
     1501 tgacctcaac aggaagaact tgacgtgctg ggcagagaac gatgtgggcc gggcagaggt
     1561 ctctgttcag gtcaacgtct ccttcccggc cagtgtgcag ctgcacacgg cggtggagat
     1621 gcaccactgg tgcatcccct tctctgtgga tgggcagccg gcaccgtctc tgcgctggct
     1681 cttcaatggc tccgtgctca atgagaccag cttcatcttc actgagttcc tggagccggc
     1741 agccaatgag accgtgcggc acgggtgtct gcgcctcaac cagcccaccc acgtcaacaa
     1801 cggcaactac acgctgctgg ctgccaaccc cttcggccag gcctccgcct ccatcatggc
     1861 tgccttcatg gacaaccctt tcgagttcaa ccccgaggac cccatccctg acactaacag
     1921 cacatctgga gacccggtgg agaagaagga cgaaacacct tttggggtct cggtggctgt
     1981 gggcctggcc gtctttgcct gcctcttcct ttctacgctg ctccttgtgc tcaacaaatg
     2041 tggacggaga aacaagtttg ggatcaaccg cccggctgtg ctggctccag aggatgggct
     2101 ggccatgtcc ctgcatttca tgacattggg tggcagctcc ctgtccccca ccgagggcaa
     2161 aggctctggg ctccaaggcc acatcatcga gaacccacaa tacttcagtg atgcctgtgt
     2221 tcaccacatc aagcgccggg acatcgtgct caagtgggag ctgggggagg gcgcctttgg
     2281 gaaggtcttc cttgctgagt gccacaacct cctgcctgag caggacaaga tgctggtggc
     2341 tgtcaaggca ctgaaggagg cgtccgagag tgctcggcag gacttccaac gtgaggctga
     2401 gctgctcacc atgctgcagc accagcacat cgtgcgcttc ttcggcgtct gcaccgaggg
     2461 ccgccccctg ctcatggtct ttgagtatat gcggcacggg gacctcaacc gcttcctccg
     2521 atcccatgga cctgatgcca agctgctggc tggtggggag gatgtggctc caggccccct
     2581 gggtctgggg cagctgctgg ccgtggctag ccaggtcgct gcggggatgg tgtacctggc
     2641 gggtctgcat tttgtgcacc gggacctggc cacacgcaac tgtctagtgg gccagggact
     2701 ggtggtcaag attggtgatt ttggcatgag cagggatatc tacagcaccg actattaccg
     2761 tgtgggaggc cgcaccatgc tgcccattcg ctggatgccg cccgagagca tcctgtaccg
     2821 taagttcacc accgagagcg acgtgtggag cttcggcgtg gtgctctggg agatcttcac
     2881 ctacggcaag cagccctggt accagctctc caacacggag gcaatcgact gcatcacgca
     2941 gggacgtgag ttggagcggc cacgtgcctg cccaccagag gtctacgcca tcatgcgggg
     3001 ctgctggcag cgggagcccc agcaacgcca cagcatcaag gatgtgcacg cccggctgca
     3061 agccctggcc caggcacctc ctgtctacct ggatgtcctg ggctaggggg ccggcccagg
     3121 ggctgggagt ggttagccgg aatactgggg cctgccctca gcatccccca tagctcccag
     3181 cagccccagg gtgatctcaa agtatctaat tcaccctcag catgtgggaa gggacaggtg
     3241 ggggctggga gtagaggatg ttcctgcttc tctaggcaag gtcccgtcat agcaattata
     3301 tttattatcc cttgaaaaaa aaggaattca gatctggtac cacgcgtatc gataagcttg
     3361 catgcctgca ggtcgactct agaggatccc gggtggcatc cctgtgaccc ctccccagtg
     3421 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     3481 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     3541 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     3601 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     3661 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     3721 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     3781 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     3841 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac
     3901 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg
     3961 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg
     4021 cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4141 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4201 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4261 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4321 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcctc
     4381 gaggaactga aaaaccagaa agttaattcc ctatagtgag tcgtattaaa ttcgtaatca
     4441 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5281 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     6601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7021 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
     7081 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7141 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7201 ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca tattagtcat
     7261 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
     7321 taatat
//
