LOCUS       sequence-222738-        8046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-Sall4
FEATURES             Location/Qualifiers
     source          1..8046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    787..811
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4052..4076)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4127..4501
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(4173..4189)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(4511..4927)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(4527..4543)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4559..4581)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4986..5343
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             5406..5999
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        6029..6133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6134..6202,6203..6994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6352..6371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7158..7746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7647..7666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7900..7917
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
       61 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
      121 tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt attgagctcg
      181 gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt caaataaggc
      241 acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct ctagaaactg
      301 ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg ggcaggaact
      361 gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc ttggccctga
      421 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
      481 tgttcctgac cttgatctga acttctctat tctcagttat gtatttttcc atgccttgca
      541 aaatggcgtt acttaagcta gcttgccaaa cctacaggtg gggtctttca ttcccccctt
      601 tttctggaga ctaaataaaa tcttttattt tatcgtcgac cactgtgctg gcggccgctc
      661 gagtttaaat agtacataat ggatttcctt acgcgaaata cgggcagaca tggcctgccc
      721 ggttattatt atttttgaca ccagaccaac tggtaatggt agcgaccggc gctcagctgg
      781 aatatcacca ctttgtacaa gaaagctggg tcggcgcgcc caccctttta gctgacagca
      841 atcttatttt cctccaggaa gtgagggaac tggtgtttgg ccatgccgtc gggaacagcg
      901 agcttttctc cgttcccgct catgggcatg ctcacaccgg tctgagagcc gtcaagcttg
      961 gaaatcgtgc cattgctcac cacagagctg gcccccagcg acacaggcag cgtggggatg
     1021 cctccgctct ggatcacgga gatctcgttg gtcttcatgg ccagaccccc attcaggacg
     1081 ctggtgtact ggttccagga ggcggggtcc acactcaccg cgggggacag gagctccttg
     1141 gaaaacacct cgggcgctct ctttccctca gcactcagcg cgaccatggg gttctctatg
     1201 gccagcttcc ttccccggcg ggcggagtta ttgttggccc catgagtcat gtagtgtacc
     1261 ttcaggttgc ctttcgtggt gaaggcccgc ccgcatatgt tacacacgaa aggcttctct
     1321 cccgtgtgtg ttcgctcgtg gatctgcagg gcactggcag acgagaagtt ctttccacac
     1381 cgtgtgcagc agtgctgctt ggcctggcgg cgtggctgcg gctgcgatgc tagcaaaggc
     1441 ggcatacccg agggcatgct ggggggtccc acaaaggtgc caggcacttc aactttgaca
     1501 aaggtgggct gtgctcggat aaatgttgga gggaggctgg tacgaccttc ggtgtcaacg
     1561 cggcagctct ccacgccctc ggccttgtga ccctcgggag accgggactt gacacttccg
     1621 gcttggctat tggcgggtga cactgcctgg tagcacatgg tctccgtggc atctgggctt
     1681 cggctctgac actcctggtc acccacgagc aaggaagagt cattggctgg accagtgtca
     1741 ccgccctcca gggagctgtt ttctcgactg ctctgcctct gcagggccag cgccggaaga
     1801 gcccctttcc cctgcgtatc cagggaggcc aacacagaga agcccagaga gggcgatgcc
     1861 aggtgggcac tgggaaccgg cagagagaca gttgaggggc cactgggaac atcctgagaa
     1921 cagacctcct cggcttccat cccttctgct gcgtcgtcct ggcagacccc gctggcactg
     1981 ccattctcac tgacggccac gggctcggga gccgtgaagt cacagggact ctctggcaga
     2041 ggggtgttgg ggatctggcc acccatgtgc atccggatat gctgctgtaa catgacggcg
     2101 ttggtgaatt tcttctggca gatggggcac gaatgttggg tctttacggt cgtgttggtt
     2161 cggtgaaccc caaggtgtgt cttcaggttg cctttggtgg agaaggcccg gccacagatc
     2221 ttgcactgga atggtctctc ccctgtgtgg gtacggtaat gcatcttcag ggaactctga
     2281 cagctgagga cccgatgaca aatgagacac tcgttggggt cagtagtggc cttgtctatg
     2341 ttctccacta gttgctgcaa tttcagggtc tcggaccctg actctggggc cccagtgccc
     2401 tggaagcccc cagcctttgg gggattatgt atcatcccca ccccaaggag tggaaggccc
     2461 gcctcacttt ctggagaagg ccctggctgc atgtcattgg gcaaggagcc acccatgagg
     2521 tccctggaat taggccctga cgtgagcttt tgaggcagcc ctagagaagg ggttcccgtg
     2581 accgggacag gctcggcgtc tacagagaga ctcgattcat cggcagggac ggggacaggg
     2641 agtgcatagt gagaagcggc actcactgcc cctttgtcct ggaattcggc caacagctgg
     2701 gggtttgcct tcacctcagg gtgtcgttgt aagtggacct tgagattgcc cttggtggtg
     2761 aagcggtgac cacagatagg gcacacgtaa ggtctctctc cggtgtggga gcgaaggtga
     2821 atctgaaggg agctatctgt cccgaaaacc ttgggacagt acctacactt gtgcttaccg
     2881 aggacgactt cgtccttggc cttgacatct aacaccgagg cagaggcgga gaggttctgg
     2941 ggtttcccct ttcctttctt ggactggtcg agcgtcacag cggagaaggg actctgcagg
     3001 agcacagagc ccggagtctg aggtagcagg gcactgggaa ggcgagacac gaagttgggg
     3061 agaacccgtg tcccgtcagg cttcaaggtg aaggacgtta accccgagga caacgggctg
     3121 gctgcggagg ggacgctggc atgaggtagc ttggcttgtt tcaaggcatc gagcgacaga
     3181 gctgggtttg aggctttctg gctgagcagg gccacggcag ccgacacctg ctgggacacg
     3241 gacacttgct gagacacatg gctgcttaag gccttcagcg tgtcggcccc cgccactcca
     3301 gagtggagcg cgtgcgctgc ccacatgttc acctggacgc gaatctgttc cgtaagctgg
     3361 atttgctgga gttgctgctg ctgcaggcac aggatctgct ccaggaccca agggatgcct
     3421 tgggcagcag gcatgggcgc catgggtgcc tctgcacccc gttggttcac ggccaccttg
     3481 gtgccgcgga gcgcctgcag agtcacattg gtgttggcta ctttgccttt gggtaaatag
     3541 cttatgtcct ggggtgtgga tggctgggta gcctctgtct tcaagtacag gatggagtcc
     3601 gtgcccagct tcttcaagtc ccccgagctg ctgccgttct cctggagact gtctttattg
     3661 cttgggctgc ccagctggtg gctcagggca gctctggaaa agtcctctga aggcactggc
     3721 ccctcgctgt cattcatgat gaggacagga ggggttttag tgcaactttt cttgtgttcc
     3781 atgaattcag agagactaaa gaactcggca cagcatttgt tgcagatgtg agtgtcctcc
     3841 cgccggggcc gcttcaccgg tatggagtcc tctgaggctt catcgcagtt cccaggggag
     3901 ttcactggag caccgggttc ctccgccgcc agcgcctcgg cgaggtcggg gctcggtagc
     3961 tgctgaggct gctcgccctg gccctcctcc cagttgatgt gctggggctt cgcctgcttg
     4021 cgcctcgaca tggtgaaggg ggcggccgcg gagcctgctt ttttgtacaa acttgtgatt
     4081 tcccgtacca ccacactggg atccttaatt aactagctag atccggcagt ctagaggatg
     4141 gtccaccccc ggggtcggca gccttcacgt gggcggcgtg tatccaagct gcgatgccgt
     4201 ctactttgag ggcggtgggg gtggtcagca ggactgtgta aggtcctttc cagcgaggtt
     4261 ctaggttctt agtctggtgt cggcggaccc acactgtgtc gccgactcgg taagggtgag
     4321 gtaccaccgg tcggtccagt tgttcttggt aggctgccgc cagaggtctc cagacttcgt
     4381 gctggactaa gtagagagcc tgtaagtgag cttggagaga ggggctgtta gtaactcttg
     4441 tcatgtcagg gtcagggaag tttacaaggg gcgggggtgc cccatataag atctcatatg
     4501 gccatatggg ggcgcctaga gaaggagtga gggctggata aagggaggat cgaggcgggg
     4561 tcgaacgagg aggttcaagg gggagagacg gggcggatgg aggaagagga ggcggaggct
     4621 tagggtgtac aaagggcttg acccagggag gggggtcaaa agccaaggct tcccaggtca
     4681 cgatgtaggg gacctggtct gggtgtccat gcgggccagg tgaaaagacc ttgatcttaa
     4741 cctgggtgat gaggtctcgg ttaaaggtgc cgtctcgcgg ccatccgacg ttaaaggttg
     4801 gccattctgc agagcagaag gtaacccaac gtctcttctt gacatctacc gactggttgt
     4861 gagcgatccg ctcgacatct ttccagtgac ctaaggtcaa acttaaggga gtggtaacag
     4921 tctggcccta attttcagac aaatacagaa acacagtcag acagagacaa cacagaacga
     4981 tgctgcagca gacaagacgc gcggcgcggc ttcggtccca aaccgaaagc aaaaattcag
     5041 acggaggcgg gaactgtttt aggttctcgt ctcctaccag aaccacatat ccctcctatt
     5101 aggggggtgc accaaagagt ccaaaacgat cgggattttt ggactcaggt cgggccacaa
     5161 aaacggcccc cgaagtccct gggacgtctc ccagggttgc ggccgggtgt tccgaactcg
     5221 tcagttccac cacgggtccg ccagatacag agctagttag ctaactagta ccgacgcagg
     5281 cgcataaaat cagtcataga cactagacaa tcggacagac acagataagt tgctggccag
     5341 cttacctccc ggtggtgggt cggtggtccc tgggcagggg tctcccgatc ccggacgagc
     5401 ccccaaatga aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg
     5461 aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc
     5521 gggcgactca gtcaatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt
     5581 gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa
     5641 ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta
     5701 gaaactgctg agggctggac cgcatctggg gaccatctgt tcttggccct gagccggggc
     5761 aggaactgct taccacagat atcctgtttg gcccatattc agctgttcca tctgttcttg
     5821 gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca tattcagctg
     5881 ttccatctgt tcctgacctt gatctgaact tttctattct cagttatgta tttttccatg
     5941 ccttgcaaaa tggcgttact taagctagct tgccaaacct acaggtgggg tctttcatta
     6001 tgcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7021 actttagatt gatttgcggc cggccgcaaa aggatctagg tgaagatcct ttttgataat
     7081 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7741 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7801 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7861 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7921 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7981 aactatggcc atttaatgta aatacttaag aaaaaaaacc aaattaattt tgatacatgc
     8041 tgcatg
//
