LOCUS       sequence-22274-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Jin J, Ang XL, Shirogane T, Wade Harper J
  TITLE     Identification of substrates for F-box proteins.
  JOURNAL   Methods Enzymol. 2005;399:287-309. doi: 
            10.1016/S0076-6879(05)99020-4.
  PUBMED    16338364
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2005"; volume: "399:287-309. doi"; pages: " 
            10.1016/S0076-6879(05)99020-4"
COMMENT     Sequence Label: pcDNA3-DN-hCUL4A-FLAG
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..59)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gactacttgt catcgtcatc cttgtagtcg
       61 aacaggatca tgatcttgtc caacgtccgc tccagctcct cgtctgtggc ttctttgttg
      121 cctgctctta actttgaatc cacatgcttt gcgatcagtt ctgcaggctt gttgggtctc
      181 ttgttgatga acgtctcaaa ggactccttc atcaggttga cgaaccgctc attcttctgg
      241 aagcagacct cgatcacgtg gtccaccttg tccttgaagt ccaacaggtc ttggaccatg
      301 tctttatctt tctcaggatt gattacgatc gctgttccaa aagtcttgat gtactcgctc
      361 cagtgctgca gcagcgcctg ctgcccgccc ctcacccggc tgaacagctg gtacatctgt
      421 gcgaggtccg gcactctgtt ctcatccagt aagtggtcga gccctttctg cagaattgct
      481 gttaaatgtt ctcctaatag ctgtttctcc acacaagcaa tcagtggttt ctgtgtgctg
      541 tggtccaagt aagtgattac tctgtctccc tcttcctcta agcgtttact tacatggtta
      601 agatattctg ggacctctct ttcctgcatt aacctttggc cttcggcagc atataagcaa
      661 ttagtctctt ccaaaaattt cagtttcaat gaatctttat acacct
//
