LOCUS       sequence-222757-       12303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for 
            iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12303)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 12303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: FUW-SOKM
FEATURES             Location/Qualifiers
     source          1..12303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2618..2634
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2676..2709)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2759..3970
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3807..3826
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4983..5039
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6117..6170
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7641..7700
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     protein_bind    complement(9102..9135)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9191..9779
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9244..9264)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9662..9673)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9782..9798)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             10308..10488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10550..11138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10630..10649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11309..12100,12101..12169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11932..11951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12170..12274)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc gctctatcga ggtcgacggt atcgataagc
     2641 tcgcttcacg agattccagc aggtcgaggg acctaataac ttcgtatagc atacattata
     2701 cgaagttata ttaagggttc caagcttaag cggcctaacc cgtgtcggct ccagatctgg
     2761 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     2821 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     2881 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     2941 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     3001 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     3061 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     3121 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     3181 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     3241 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     3301 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     3361 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     3421 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     3481 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     3541 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     3601 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     3661 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     3721 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     3781 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     3841 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     3901 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     3961 tttgttagac gaagcttggg ctgcaggtcg actctagaga attcgccacc atgtataaca
     4021 tgatggagac ggagctgaag ccgccgggcc cgcagcaagc ttcggggggc ggcggcggag
     4081 gaggcaacgc cacggcggcg gcgaccggcg gcaaccagaa gaacagcccg gaccgcgtca
     4141 agaggcccat gaacgccttc atggtatggt cccgggggca gcggcgtaag atggcccagg
     4201 agaaccccaa gatgcacaac tcggagatca gcaagcgcct gggcgcggag tggaaacttt
     4261 tgtccgagac cgagaagcgg ccgttcatcg acgaggccaa gcggctgcgc gctctgcaca
     4321 tgaaggagca cccggattat aaataccggc cgcggcggaa aaccaagacg ctcatgaaga
     4381 aggataagta cacgcttccc ggaggcttgc tggcccccgg cgggaacagc atggcgagcg
     4441 gggttggggt gggcgccggc ctgggtgcgg gcgtgaacca gcgcatggac agctacgcgc
     4501 acatgaacgg ctggagcaac ggcagctaca gcatgatgca ggagcagctg ggctacccgc
     4561 agcacccggg cctcaacgct cacggcgcgg cacagatgca accgatgcac cgctacgacg
     4621 tcagcgccct gcagtacaac tccatgacca gctcgcagac ctacatgaac ggctcgccca
     4681 cctacagcat gtcctactcg cagcagggca cccccggtat ggcgctgggc tccatgggct
     4741 ctgtggtcaa gtccgaggcc agctccagcc cccccgtggt tacctcttcc tcccactcca
     4801 gggcgccctg ccaggccggg gacctccggg acatgatcag catgtacctc cccggcgccg
     4861 aggtgccgga gcccgctgcg cccagtagac tgcacatggc ccagcactac cagagcggcc
     4921 cggtgcccgg cacggccatt aacggcacac tgcccctgtc gcacatggct agcggcagcg
     4981 gcgccacgaa cttctctctg ttaaagcaag caggagatgt tgaagaaaac cccgggcctg
     5041 catgcatggc tggacacctg gcttcagact tcgccttctc acccccacca ggtgggggtg
     5101 atgggtcagc agggctggag ccgggctggg tggatcctcg aacctggcta agcttccaag
     5161 ggcctccagg tgggcctgga atcggaccag gctcagaggt attggggatc tccccatgtc
     5221 cgcccgcata cgagttctgc ggagggatgg catactgtgg acctcaggtt ggactgggcc
     5281 tagtccccca agttggcgtg gagactttgc agcctgaggg ccaggcagga gcacgagtgg
     5341 aaagcaactc agagggaacc tcctctgagc cctgtgccga ccgccccaat gccgtgaagt
     5401 tggagaaggt ggaaccaact cccgaggagt cccaggacat gaaagccctg cagaaggagc
     5461 tagaacagtt tgccaagctg ctgaagcaga agaggatcac cttggggtac acccaggccg
     5521 acgtggggct caccctgggc gttctctttg gaaaggtgtt cagccagacc accatctgtc
     5581 gcttcgaggc cttgcagctc agccttaaga acatgtgtaa gctgcggccc ctgctggaga
     5641 agtgggtgga ggaagccgac aacaatgaga accttcagga gatatgcaaa tcggagaccc
     5701 tggtgcaggc ccggaagaga aagcgaacta gcattgagaa ccgtgtgagg tggagtctgg
     5761 agaccatgtt tctgaagtgc ccgaagccct ccctacagca gatcactcac atcgccaatc
     5821 agcttgggct agagaaggat gtggttcgag tatggttctg taaccggcgc cagaagggca
     5881 aaagatcaag tattgagtat tcccaacgag aagagtatga ggctacaggg acacctttcc
     5941 cagggggggc tgtatccttt cctctgcccc caggtcccca ctttggcacc ccaggctatg
     6001 gaagccccca cttcaccaca ctctactcag tcccttttcc tgagggcgag gcctttccct
     6061 ctgttcccgt cactgctctg ggctctccca tgcattcaaa cgcatgcggc tccggcgagg
     6121 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcgagatga
     6181 ggcagccacc tggcgagtct gacatggctg tcagcgacgc tctgctcccg tccttctcca
     6241 cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt gccccgacta
     6301 accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc ggccgcccct
     6361 acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt gcagcttgca
     6421 gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac gacctcctgg
     6481 acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc gccaccgtga
     6541 ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct gccagcgcgc
     6601 cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc gtggctgcca
     6661 gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc acggccccct
     6721 tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct gagctcctgc
     6781 ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca ggtggcgggc
     6841 tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag tacagcagcc
     6901 cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg gtagtggcgc
     6961 cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg gtcccgtcct
     7021 gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc agcaacggcc
     7081 accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg actaccccta
     7141 cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg cctcttcccc
     7201 caggattcca tccccatccg gggcccaact accctccttt cctgccagac cagatgcagt
     7261 cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc ctgccagagg
     7321 agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc acccacactt
     7381 gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag gcacacctgc
     7441 gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg tggaaattcg
     7501 cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg ccctttcagt
     7561 gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac atgaagaggc
     7621 actttctcga gggctcgggc cagtgtacta attatgctct cttgaaattg gctggagatg
     7681 ttgagagcaa cccaggtccc ggcgcgccac tggatttcct ttgggcgttg gaaaccccgc
     7741 agacagccac gacgatgccc ctcaacgtga acttcaccaa caggaactat gacctcgact
     7801 acgactccgt acagccctat ttcatctgcg acgaggaaga gaatttctat caccagcaac
     7861 agcagagcga gctgcagccg cccgcgccca gtgaggatat ctggaagaaa ttcgagctgc
     7921 ttcccacccc gcccctgtcc ccgagccgcc gctccgggct ctgctctcca tcctatgttg
     7981 cggtcgctac gtccttctcc ccaagggaag acgatgacgg cggcggtggc aacttctcca
     8041 ccgccgatca gctggagatg atgaccgagt tacttggagg agacatggtg aaccagagct
     8101 tcatctgcga tcctgacgac gagaccttca tcaagaacat catcatccag gactgtatgt
     8161 ggagcggttt ctcagccgct gccaagctgg tctcggagaa gctggcctcc taccaggctg
     8221 cgcgcaaaga cagcaccagc ctgagccccg cccgcgggca cagcgtctgc tccacctcca
     8281 gcctgtacct gcaggacctc accgccgccg cgtccgagtg cattgacccc tcagtggtct
     8341 ttccctaccc gctcaacgac agcagctcgc ccaaatcctg tacctcgtcc gattccacgg
     8401 ccttctctcc ttcctcggac tcgctgctgt cctccgagtc ctccccacgg gccagccctg
     8461 agcccctagt gctgcatgag gagacaccgc ccaccaccag cagcgactct gaagaagagc
     8521 aagaagatga ggaagaaatt gatgtggtgt ctgtggagaa gaggcaaacc cctgccaaga
     8581 ggtcggagtc gggctcatct ccatcccgag gccacagcaa acctccgcac agcccactgg
     8641 tcctcaagag gtgccacgtc tccactcacc agcacaacta cgccgcaccc ccctccacaa
     8701 ggaaggacta tccagctgcc aagagggcca agttggacag tggcagggtc ctgaagcaga
     8761 tcagcaacaa ccgcaagtgc tccagcccca ggtcctcaga cacggaggaa aacgacaaga
     8821 ggcggacaca caacgtcttg gaacgtcaga ggaggaacga gctgaagcgc agcttttttg
     8881 ccctgcgtga ccagatccct gaattggaaa acaacgaaaa ggcccccaag gtagtgatcc
     8941 tcaaaaaagc caccgcctac atcctgtcca ttcaagcaga cgagcacaag ctcacctctg
     9001 aaaaggactt attgaggaaa cgacgagaac agttgaaaca caaactcgaa cagcttcgaa
     9061 actctggtgc ataaggcgcg ccgaattcgt cgagggacct aataacttcg tatagcatac
     9121 attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa
     9181 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     9241 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     9301 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     9361 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     9421 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     9481 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     9541 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     9601 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     9661 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     9721 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     9781 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     9841 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     9901 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     9961 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    10021 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
    10081 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
    10141 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
    10201 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
    10261 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
    10321 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10381 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10441 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa
    10501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    12061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    12121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12301 acg
//
