LOCUS       sequence-22276-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Jin J, Ang XL, Shirogane T, Wade Harper J
  TITLE     Identification of substrates for F-box proteins.
  JOURNAL   Methods Enzymol. 2005;399:287-309. doi: 
            10.1016/S0076-6879(05)99020-4.
  PUBMED    16338364
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2005"; volume: "399:287-309. doi"; pages: " 
            10.1016/S0076-6879(05)99020-4"
COMMENT     Sequence Label: pcDNA3-DN-hCUL5-FLAG
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..59)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gactacttgt catcgtcatc cttgtagtca
       61 agtaccaaga gcacttcttt aagctttgct tcaatctctt cagaggttag ttttttgctt
      121 aatggtgttt ttcttagcaa catgtcacag taattggcaa gcagctcagg gcattttgat
      181 tcaggctgag tttttaatcc cacccccttc tgcttcaaag gtaattcaag tttaaatatg
      241 gtagcatcat taacaactgc tttatacgcc ttatctcttg cagtaagaaa tcgtggatca
      301 tcttgaaaag cttctttgac gagtttacta aatctattaa atagtgtaag taactgctca
      361 acgtatttct cagagtcagt agtaatagtt tcagcagctg ctaccatatc tgccaggcca
      421 gcactaatga tatgttcctc caagtctttc aacattggct ctataccatt aggaactttg
      481 tccatcaatg aaaacattaa atgtaatttt tcagtttcat ttctcttgat catgccttgg
      541 cactcagcta agatagtctc tttaaatgat gtcaccaggg catttacaca gcattccatg
      601 agtgcttcaa cggagttaca ttctcgtctt gtttctaaat aacgtagtgc tcgtttttct
      661 tcttctttta atttagcatc tgcatatttc atataattct gtacaccatt ttgttgtaaa
      721 tacgagggtg cttgtgttct ataaaatctc tctgttgaat caagtatgcc ttct
//
