LOCUS       sequence-222767-        7623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Yan Q, Bartz S, Mao M, Li L, Kaelin WG
  TITLE     The hypoxia-inducible factor 2alpha N-terminal and C-terminal 
            transactivation domains cooperate to promote renal tumorigenesis in 
            vivo.
  JOURNAL   Mol Cell Biol. 2007 Mar . 27(6):2092-102.
  PUBMED    17220275
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Mar . 27(6):2092-102."
COMMENT     Sequence Label: HA-HIF1alpha P402A/P564A-pBabe-puro
FEATURES             Location/Qualifiers
     source          1..7623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             13..482
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    547..746
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             947..1363
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1293..1315
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1331..1347
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1395..1421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1395..1421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1401..1427
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      1434..1443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3958..3978)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3963..4292
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4143..4278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4302..4901
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4302..4321)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4798..4818
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5122..5591
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5721..5738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5892..6480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5972..5991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6640..7431,7432..7500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7263..7282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7501..7605)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgca gcctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
       61 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
      121 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
      181 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
      241 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
      301 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
      361 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
      421 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
      481 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
      541 ggaggcaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga
      601 ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg
      661 tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg
      721 gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg
      781 atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt
      841 gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct
      901 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac
      961 cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca
     1021 gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt
     1081 taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat
     1141 caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg
     1201 ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc
     1261 gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc
     1321 tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggccgga tcgaagacta
     1381 agcttaccat ggcctacccc tacgacgtgc ccgactacgc ctccctcgga tccgccacca
     1441 tggagggcgc cggcggcgcg aacgacaaga aaaagataag ttctgaacgt cgaaaagaaa
     1501 agtctcgaga tgcagccaga tctcggcgaa gtaaagaatc tgaagttttt tatgagcttg
     1561 ctcatcagtt gccacttcca cataatgtga gttcgcatct tgataaggcc tctgtgatga
     1621 ggcttaccat cagctatttg cgtgtgagga aacttctgga tgctggtgat ttggatattg
     1681 aagatgacat gaaagcacag atgaattgct tttatttgaa agccttggat ggttttgtta
     1741 tggttctcac agatgatggt gacatgattt acatttctga taatgtgaac aaatacatgg
     1801 gattaactca gtttgaacta actggacaca gtgtgtttga ttttactcat ccatgtgacc
     1861 atgaggaaat gagagaaatg cttacacaca gaaatggcct tgtgaaaaag ggtaaagaac
     1921 aaaacacaca gcgaagcttt tttctcagaa tgaagtgtac cctaactagc cgaggaagaa
     1981 ctatgaacat aaagtctgca acatggaagg tattgcactg cacaggccac attcacgtat
     2041 atgataccaa cagtaaccaa cctcagtgtg ggtataagaa accacctatg acctgcttgg
     2101 tgctgatttg tgaacccatt cctcacccat caaatattga aattccttta gatagcaaga
     2161 ctttcctcag tcgacacagc ctggatatga aattttctta ttgtgatgaa agaattaccg
     2221 aattgatggg atatgagcca gaagaacttt taggccgctc aatttatgaa tattatcatg
     2281 ctttggactc tgatcatctg accaaaactc atcatgatat gtttactaaa ggacaagtca
     2341 ccacaggaca gtacaggatg cttgccaaaa gaggtggata tgtctgggtt gaaactcaag
     2401 caactgtcat atataacacc aagaattctc aaccacagtg cattgtatgt gtgaattacg
     2461 ttgtgagtgg tattattcag cacgacttga ttttctccct tcaacaaaca gaatgtgtcc
     2521 ttaaaccggt tgaatcttca gatatgaaaa tgactcagct attcaccaaa gttgaatcag
     2581 aagatacaag tagcctcttt gacaaactta agaaggaacc tgatgcttta actttgctgg
     2641 ccgcagccgc tggagacaca atcatatctt tagattttgg cagcaacgac acagaaactg
     2701 atgaccagca acttgaggaa gtaccattat ataatgatgt aatgctcccc tcacccaacg
     2761 aaaaattaca gaatataaat ttggcaatgt ctccattacc caccgctgaa acgccaaagc
     2821 cacttcgaag tagtgctgac cctgcactca atcaagaagt tgcattaaaa ttagaaccaa
     2881 atccagagtc actggaactt tcttttacca tgccccagat tcaggatcag acacctagtc
     2941 cttccgatgg aagcactaga caaagttcac ctgagcctaa tagtcccagt gaatattgtt
     3001 tttatgtgga tagtgatatg gtcaatgaat tcaagttgga attggtagaa aaactttttg
     3061 ctgaagacac agaagcaaag aacccatttt ctactcagga cacagattta gacttggaga
     3121 tgttagctgc ttatatccca atggatgatg acttccagtt acgttccttc gatcagttgt
     3181 caccattaga aagcagttcc gcaagccctg aaagcgcaag tcctcaaagc acagttacag
     3241 tattccagca gactcaaata caagaaccta ctgctaatgc caccactacc actgccacca
     3301 ctgatgaatt aaaaacagtg acaaaagacc gtatggaaga cattaaaata ttgattgcat
     3361 ctccatctcc tacccacata cataaagaaa ctactagtgc cacatcatca ccatatagag
     3421 atactcaaag tcggacagcc tcaccaaaca gagcaggaaa aggagtcata gaacagacag
     3481 aaaaatctca tccaagaagc cctaacgtgt tatctgtcgc tttgagtcaa agaactacag
     3541 ttcctgagga agaactaaat ccaaagatac tagctttgca gaatgctcag agaaagcgaa
     3601 aaatggaaca tgatggttca ctttttcaag cagtaggaat tggaacatta ttacagcagc
     3661 cagacgatca tgcagctact acatcacttt cttggaaacg tgtaaaagga tgcaaatcta
     3721 gtgaacagaa tggaatggag caaaagacaa ttattttaat accctctgat ttagcatgta
     3781 gactgctggg gcaatcaatg gatgaaagtg gattaccaca gctgaccagt tatgattgtg
     3841 aagttaatgc tcctatacaa ggcagcagaa acctactgca gggtgaagaa ttactcagag
     3901 ctttggatca agttaactga caattctgca gatatccatc acagtggtcg accctgtgga
     3961 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4021 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4081 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4141 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4201 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4261 gaggcttttt tggaggccta ggcttttgca aaaagcttac catgaccgag tacaagccca
     4321 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     4381 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     4441 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     4501 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     4561 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     4621 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     4681 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     4741 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     4801 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     4861 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     4921 agcgcccgac cgaaaggagc gcacgacccc atgcatcgat aaaataaaag attttattta
     4981 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     5041 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     5101 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     5161 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     5221 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     5281 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     5341 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     5401 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     5461 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     5521 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     5581 ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac
     5641 attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6301 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa
     6601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7621 agt
//
