LOCUS       sequence-222775-       10859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: E[beta]C
FEATURES             Location/Qualifiers
     source          1..10859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..67
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    184..205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(220..225,226..243,244..250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    258..274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     263..285
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     282..298
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     282..298
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     317..337
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        319..337
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        365..591
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             592..772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    819..944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1437..1670
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1855..1899
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2166..2282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2318..3496
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2549..3487
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3444..3464
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3513..3531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3657..3692
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     primer_bind     complement(6006..6026)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6011..6340
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6191..6326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6350..7060
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6495..6513)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6763..6782
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7067..7655
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7120..7140)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7538..7549)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7742..7975
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8047..8168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8084..8103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8138..8157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8208..8343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8364..8382)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8392..8409)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8392..8408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8401..8423)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8550..9005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8637..8656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8847..8868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9031..9135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9136..9204,9205..9996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9354..9373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10167..10755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10656..10675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
       61 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      121 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      181 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      241 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      301 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
      361 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
      421 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      481 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      541 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      601 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      661 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      721 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      781 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      841 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      901 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      961 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1021 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1081 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1141 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1201 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1261 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1321 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1381 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1441 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1501 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1561 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1621 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1681 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1741 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1801 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1861 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1921 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1981 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2041 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2101 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     2161 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2221 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
     2281 ttatcggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     2341 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     2401 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2461 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2521 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     2581 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     2641 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     2701 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     2761 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     2821 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     2881 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     2941 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     3001 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     3061 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     3121 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     3181 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     3241 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     3301 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     3361 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     3421 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     3481 atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa
     3541 gctggctagg taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg
     3601 cagataacac catggctact caagctgacc tgatggagtt ggacatggcc atggagccgg
     3661 acagaaaagc tgctgtcagc cactggcagc agcagtctta cttggatgct ggaatccatg
     3721 ctggtgccac cgccacagct cctgccctga gtggcaaggg caaccctgag gaagaagatg
     3781 ttgacacctc ccaagtcctt tatgaatggg agcaaggctt ttcccagtcc ttcacgcaag
     3841 agcaagtagc tgatattgac gggcagtatg caatgactag ggctcagagg gtccgagctg
     3901 ccatgttccc tgagacgcta gatgagggca tgcagatccc atccacgcag tttgacgctg
     3961 ctcatcccac taatgtccag cgcttggctg aaccatcaca gatgttgaaa catgcagttg
     4021 tcaatttgat taactatcag gatgacgcgg aacttgccac acgtgcaatt cctgagctga
     4081 caaaactgct aaacgatgag gaccaggtgg tagttaataa agctgctgtt atggtccatc
     4141 agctttccaa aaaggaagct tccagacatg ccatcatgcg ctcccctcag atggtgtctg
     4201 ccattgtacg caccatgcag aatacaaatg atgtagagac agctcgttgt actgctggga
     4261 ctctgcacaa cctttctcac caccgcgagg gcttgctggc catctttaag tctggtggca
     4321 tcccagcgct ggtgaaaatg cttgggtcac cagtggattc tgtactgttc tacgccatca
     4381 cgacactgca taatctcctg ctccatcagg aaggagctaa aatggcagtg cgcctagctg
     4441 gtggactgca gaaaatggtt gctttgctca acaaaacaaa cgtgaaattc ttggctatta
     4501 caacagactg ccttcagatc ttagcttatg gcaatcaaga gagcaagctc atcattctgg
     4561 ccagtggtgg accccaagcc ttagtaaaca taatgaggac ctacacttat gagaagcttc
     4621 tgtggaccac aagcagagtg ctgaaagtgc tgtctgtctg ctctagcaac aagccggcca
     4681 ttgtagaagc tggtgggatg caggcactgg ggcttcatct gacagaccca agtcagcgac
     4741 ttgttcaaaa ctgtctttgg actctcagaa acctttcaga tgcagcgact aagcaggaag
     4801 ggatggaagg cctccttggg actctagtgc agcttctggg ttccgatgat ataaatgtgg
     4861 tcacctgtgc agctggaatt ctctctaacc tcacttgcaa taattacaaa aacaagatga
     4921 tggtgtgcca agtgggtggc atagaggctc ttgtacgcac cgtccttcgt gctggtgaca
     4981 gggaagacat cactgagcct gccatctgtg ctcttcgtca tctgaccagc cggcatcagg
     5041 aagccgagat ggcccagaat gccgttcgcc ttcattatgg actgcctgtt gtggttaaac
     5101 tcctgcaccc accatcccac tggcctctga taaaggcaac tgttggattg attcgaaacc
     5161 ttgccctttg cccagcaaat catgcgcctt tgcgggaaca gggtgctatt ccacgactag
     5221 ttcagctgct tgtacgagca catcaggaca cccaacggcg cacctccatg ggtggaacgc
     5281 agcagcagtt tgtggagggc gtgcgcatgg aggagatagt agaagggtgt actggagctc
     5341 tccacatcct tgctcgggac gttcacaacc ggattgtaat ccgaggactc aataccattc
     5401 cattgtttgt gcagttgctt tattctccca ttgaaaatat ccaaagagta gctgcagggg
     5461 tcctctgtga acttgctcag gacaaggagg ctgcagaggc cattgaagct gagggagcca
     5521 cagctcccct gacagagtta ctccactcca ggaatgaagg cgtggcaaca tacgcagctg
     5581 ctgtcctatt ccgaatgtct gaggacaagc cacaggatta caagaagcgg ctttcagtcg
     5641 agctgaccag ttccctcttc aggacagagc caatggcttg gaatgagact gcagatcttg
     5701 gactggacat tggtgcccag ggagaagccc ttggatatcg ccaggatgat cccagctacc
     5761 gttcttttca ctctggtgga tacggccagg atgccttggg gatggaccct atgatggagc
     5821 atgagatggg tggccaccac cctggtgctg actatccagt tgatgggctg cctgatctgg
     5881 gacacgccca ggacctcatg gatgggctgc ccccaggtga tagcaatcag ctggcctggt
     5941 ttgatactga cctgtaatct agaggatccg gactagttaa cgcgtcgacc agtggtcgac
     6001 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tggtgagcaa
     6361 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     6421 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     6481 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     6541 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     6601 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     6661 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     6721 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     6781 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     6841 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     6901 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     6961 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     7021 ccgccactcc accggcggca tggacgagct gtacaagtaa gtcgacaatc aacctctgga
     7081 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7141 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7201 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7261 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7321 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7381 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7441 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7501 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7561 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7621 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta
     7681 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7741 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7801 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7861 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7921 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7981 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8041 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8101 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8161 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     8221 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8281 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8341 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     8401 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8461 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8521 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8581 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8641 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8701 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8761 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8821 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8881 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8941 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     9001 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9061 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattac
//
