LOCUS       sequence-222781-       14323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14323)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 14323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S ET2K
FEATURES             Location/Qualifiers
     source          1..14323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..1175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          244..1166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    2385..2958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2408..2960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    3977..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4644..5806
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4875..5797
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5944..8070
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     misc_feature    8156..8729
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8179..8731
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    10216..10350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      11083..12872
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
       61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg
      601 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
      841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct
      901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
      961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1081 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
     1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
     1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
     1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
     1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
     1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
     1501 gacctctcag cctgagggtg aagcaggagt cggggtggag agcaactccg atggggcctc
     1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
     1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
     1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
     1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
     1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
     1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
     1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
     1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
     2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
     2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
     2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
     2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
     2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
     2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
     2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
     3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
     3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
     3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
     3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
     3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
     3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
     3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
     3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
     3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
     3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
     3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
     3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
     3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
     3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
     3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
     3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4321 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgacc
     4381 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
     4441 ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat
     4501 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
     4561 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca
     4621 ttgattattg actagcttcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     4681 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     4741 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     4801 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     4861 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     4921 atggcccttg cgtgccttga attacttcca cctggctcca gtacgtgatt cttgatcccg
     4981 agctggagcc aggggcgggc cttgcgcttt aggagcccct tcgcctcgtg cttgagttga
     5041 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     5101 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     5161 tttctggcaa gatagtcttg taaatgcggg ccaggatctg cacactggta tttcggtttt
     5221 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     5281 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     5341 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     5401 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctccag ggggctcaaa
     5461 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaggggc
     5521 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     5581 cctcgattag ttctggagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     5641 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     5701 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     5761 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaacac gtggtcgcgg
     5821 ccaattctgc agtcgagctc aagcttcgaa ctagggccgc gactctagcg ctaccggact
     5881 cagatctcga gctcaagctt cgaattcgat tgatcccgcg accggtcgac cagctttgca
     5941 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     6001 gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa aaaatgcaag
     6061 gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa tactctgtac
     6121 aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg cttctgggat
     6181 gcaactgaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     6241 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     6301 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     6361 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     6421 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     6481 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     6541 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     6601 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     6661 ccattttctg ttattgagga aagtttgcca ggtgggttaa aggagcatga ttttaatcca
     6721 gaagaagcag aggaaactaa acaagtgtcc tggaagcttg taacagagta tgcaatggaa
     6781 acaaaatgtg atgatgtgtt gttattgctt gggatgtact tggaatttca gtacagtttt
     6841 gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc actataagta ccatgaaaag
     6901 cattatgcaa atgctgctat atttgctgac agcaaaaacc aaaaaaccat atgccaacag
     6961 gctgttgata ctgttttagc taaaaagcgg gttgatagcc tacaattaac tagagaacaa
     7021 atgttaacaa acagatttaa tgatcttttg gataggatgg atataatgtt tggttctaca
     7081 ggctctgctg acatagaaga atggatggct ggagttgctt ggctacactg tttgttgccc
     7141 aaaatggatt cagtggtgta tgacttttta aaatgcatgg tgtacaacat tcctaaaaaa
     7201 agatactggc tgtttaaagg accaattgat agtggtaaaa ctacattagc agctgctttg
     7261 cttgaattat gtggggggaa agctttaaat gttaatttgc ccttggacag gctgaacttt
     7321 gagctaggag tagctattga ccagttttta gtagtttttg aggatgtaaa gggcactgga
     7381 ggggagtcca gagatttgcc ttcaggtcag ggaattaata acctggacaa tttaagggat
     7441 tatttggatg gcagtgttaa ggtaaactta gaaaagaaac acctaaataa aagaactcaa
     7501 atatttcccc ctggaatagt caccatgaat gagtacagtg tgcctaaaac actgcaggcc
     7561 agatttgtaa aacaaataga ttttaggccc aaagattatt taaagcattg cctggaacgc
     7621 agtgagtttt tgttagaaaa gagaataatt caaagtggca ttgctttgct tcttatgtta
     7681 atttggtaca gacctgtggc tgagtttgct caaagtattc agagcagaat tgtggagtgg
     7741 aaagagagat tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa gtttaatgtg
     7801 gctatgggaa ttggagtttt agattggcta agaaacagtg atgatgatga tgaagacagc
     7861 caggaaaatg ctgataaaaa tgaagatggt ggggagaaga acatggaaga ctcagggcat
     7921 gaaacaggca ttgattcaca gtcccaaggc tcatttcagg cccctcagtc ctcacagtct
     7981 gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     8041 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacgggga
     8101 tcaatcacta gagtcgcggc cgctttctcg aggccatcga tgtttaaacg atccgcccct
     8161 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     8221 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     8281 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     8341 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     8401 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     8461 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     8521 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     8581 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     8641 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     8701 tggttttcct ttgaaaaaca cgatgataat atggccacac cgcgatcgcg aattcgcgat
     8761 cgccatggct gtcagcgacg cgctgctccc atctttctcc acgttcgcgt ctggcccggc
     8821 gggaagggag aagacactgc gtcaagcagg tgccccgaat aaccgctggc gggaggagct
     8881 ctcccacatg aagcgacttc ccccagtgct tcccggccgc ccctatgacc tggcggcggc
     8941 gaccgtggcc acagacctgg agagcggcgg agccggtgcg gcttgcggcg gtagcaacct
     9001 ggcgccccta cctcggagag agaccgagga gttcaacgat ctcctggacc tggactttat
     9061 tctctccaat tcgctgaccc atcctccgga gtcagtggcc gccaccgtgt cctcgtcagc
     9121 gtcagcctcc tcttcgtcgt cgccgtcgag cagcggccct gccagcgcgc cctccacctg
     9181 cagcttcacc tatccgatcc gggccgggaa cgacccgggc gtggcgccgg gcggcacggg
     9241 cggaggcctc ctctatggca gggagtccgc tccccctccg acggctccct tcaacctggc
     9301 ggacatcaac gacgtgagcc cctcgggcgg cttcgtggcc gagctcctgc ggccagaatt
     9361 ggacccggtg tacattccgc cgcagcagcc gcagccgcca ggtggcgggc tgatgggcaa
     9421 gttcgtgctg aaggcgtcgc tgagcgcccc tggcagcgag tacggcagcc cgtcggtcat
     9481 cagcgtcagc aaaggcagcc ctgacggcag ccacccggtg gtggtggcgc cctacaacgg
     9541 cgggccgccg cgcacgtgcc ccaagatcac gcaggaggcg gtctcttcgt gcacccactt
     9601 gggcgctgga ccccctctca gcaatggcca ccggccggct gcacacgact tccccctggg
     9661 gcggcagctc cccagcagga ctaccccgac cctgggtctt gaggaagtgc tgagcagcag
     9721 ggactgtcac cctgccctgc cgcttcctcc cggcttccat ccccacccgg ggcccaatta
     9781 cccatccttc ctgcccgatc agatgcagcc gcaagtcccg ccgctccatt accaagagct
     9841 catgccaccc ggttcctgca tgccagagga gcccaagcca aagaggggaa gacgatcgtg
     9901 gccccggaaa aggaccgcca cccacacttg tgattacgcg ggctgcggca aaacctacac
     9961 aaagagttcc catctcaagg cacacctgcg aacccacaca ggtgagaaac cttaccactg
    10021 tgactgggac ggctgtggat ggaaattcgc ccgctcagat gaactgacca ggcactaccg
    10081 taaacacacg gggcaccgcc cgttccagtg ccaaaaatgc gaccgagcat tttccaggtc
    10141 ggaccacctc gccttacaca tgaagaggca tttttaaatc ccagacagtg gatatgaccc
    10201 gtttctagtt cgaaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
    10261 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
    10321 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
    10381 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
    10441 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
    10501 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    10561 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgatcg
    10621 actctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg
    10681 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact
    10741 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta
    10801 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga
    10861 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg
    10921 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga
    10981 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag
    11041 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt
    11101 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac
    11161 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc
    11221 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga
    11281 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga
    11341 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga
    11401 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa
    11461 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac
    11521 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa
    11581 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct
    11641 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc
    11701 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt
    11761 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg
    11821 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg
    11881 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg
    11941 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca
    12001 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg
    12061 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca
    12121 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg
    12181 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
    12241 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    12301 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
    12361 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
    12421 gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta
    12481 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
    12541 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
    12601 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
    12661 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
    12721 atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat
    12781 atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg
    12841 ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt
    12901 aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg
    12961 ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc
    13021 caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca
    13081 acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt
    13141 aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc
    13201 tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac
    13261 cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct
    13321 gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac
    13381 ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag
    13441 cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt
    13501 aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt
    13561 actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac
    13621 gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga
    13681 acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc
    13741 ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat
    13801 cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata
    13861 atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg
    13921 agacccggat gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg
    13981 acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg
    14041 gcggcctcca ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact
    14101 gcctcctcga ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc
    14161 tsctcctgcc cctcctsccc ctcctgctcc tgcccctcct scccctcctg ctcctgcccc
    14221 tcctgcccct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc
    14281 tcctcctgct cctscccctc ctccccctcc tcctcctccc cct
//
