LOCUS       sequence-22279-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Jager S, Handschin C, St-Pierre J, Spiegelman BM
  TITLE     AMP-activated protein kinase (AMPK) action in skeletal muscle via 
            direct phosphorylation of PGC-1alpha.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jul 17. 104(29):12017-22.
  PUBMED    17609368
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Jul 17. 104(29):12017-22."
COMMENT     Sequence Label: pcDNA Flag PGC1 T177A S538A
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggggcattag cagagctctc tggctactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattctgca gatatccgga tcaccaccat ggactacaaa
      181 gacgatgacg ataaagcaag aagagcatct gtgggatcct gcagccaaga ctctgtatgg
      241 agtgacatag agtgtgctgc tctggttggt gaggaccagc ctctttgccc agatcttcct
      301 gaacttgacc tttctgaact tgatgtgaat gacttggata cagacagctt tctgggtgga
      361 ttgaagtggt gtagcgacca atcggaaatc atatccaacc agtacaacaa tgagcctgcg
      421 aacatatttg agaagataga tgaagagaat gaggcaaact tgctagcggt cctcacagag
      481 acactggaca gtctccccgt ggatgaagac ggattgccct catttgatgc actgacagat
      541 ggagccgtga ccactgacaa cgaggccagt ccttcctcca tgcctgacgg cacccctccc
      601 cctcaggagg cagaagagcc gtctctactt aagaagctct tactggcacc agccaacact
      661 cagctcagct acaatgaatg cagcggtctt agcactcana ancatgcngc aaaccacccc
      721 cncccgntgg nancnaanca anccctgcct tngttaaaac ngaaaatgcn tggancaata
      781 agccgaaaag cnttngtc
//
