LOCUS       sequence-222805-       12063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, EGFP membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Icha J, Kunath C, Rocha-Martins M, Norden C
  TITLE     Independent modes of ganglion cell translocation ensure correct 
            lamination of the zebrafish retina.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):259-275.
  PUBMED    27810916
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "259-275"
COMMENT     Sequence Label: ath5:GFP-CAAX
FEATURES             Location/Qualifiers
     source          1..12063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1346..1364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1389..1405
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1390..1406
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1440..1456)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6392..6408)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6445..6463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6463..6487
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6493..6495,6496..6498,6499..7209)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6493..6495,6496..6498,6499..7209)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6538..6559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6799..6818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7146..7167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7289..7313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7318..7452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7342..7361)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7396..7415
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9593..9780,9781..9792)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9826..9842)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9879..9897)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9918..9934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9918..9934)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9931..9953)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9942..9958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9966..9972,9973..9990,9991..9996))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10011..10032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10149..10166)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10320..10908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10400..10419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11079..11870,11871..11939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11702..11721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11940..12044)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtaccgg
     1381 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     1441 atccactagt tctagaattt aaatatttta aaaatgtata tatataataa atgtatatat
     1501 atataaatgt ataatgagca aaattctaag ttttatgcaa ataaaaaaag tattatggaa
     1561 aatgtattat attgcagttc taaattattt aagaaaacgt ggctgacttg ccaaaatgat
     1621 ggtaaactgt gcaactctgc ttccaagatg ttagaaacag acgtttttct tttagcctca
     1681 taatttaatg ctgttgcgat gttaccgtct cttcgctgta ctggttgtta ccaggttacg
     1741 gaaaaatata acatgctcac aacatccaat cagattaaat gaactgaaaa gcaatatggg
     1801 ttttaagaca tcacagagaa ggtaacattt aaaggcttaa aagcacaaac tgtttctcgc
     1861 ttctaagact aagtaagtaa gtttttgcac acaattaaca aatagttgca ggcaaattaa
     1921 actttagtga caaggcagta ctggcccgat caggccagta atgattttgt ctactgtcca
     1981 aagcatctct cacgctggcc ctgggtcatc aagcagttct tattgttgag ccctgcatta
     2041 taattatata caattttttt tttattattt aattttttta aattaaaatg ttatgtaatg
     2101 ttatgttaat aaatgtttaa ataagaatat taaaaaataa tttgatttaa tacaaataca
     2161 gggaagcacg ttttaaaaag taaataaatt ttgttaaatt ttatataagg aaaatatgtt
     2221 tatttctgta tttttctata cactgaacac aactaagaat ttctaaataa atatagacaa
     2281 atgctattag gcatgggatg ataaccagtt taaaggtcaa ggtttaaaaa cccccagaat
     2341 tatctgttat accgttccaa cgatatatgt aattttttta ttaacacttt taataaaaaa
     2401 ggaccctcat catcaaactg ttcttaaaat aaactatatt gtgctcaaag gtaaaaacag
     2461 ttcttgtttt ttacccagac atttaaaaaa agaatttatt ttagagcagt aatcacaata
     2521 ccgtccgtga taccgtgatt tttttttatc aaaggtaatc atacagtcac aatcttttac
     2581 cggcccatgc tatttatacg gcagtgtaaa ataaagtgta aattgtaacg cgttataaag
     2641 aattgcttct tttaaacaat gccacaaaaa ttatgaacat tttttttttg taaaaatttt
     2701 aaaccaaaaa taactaaaaa tttctccaaa tataaaacaa ttgagcaggg cacattgtaa
     2761 cacataacga aaactgcttc cattgttttt ttttatactt gaaaatcctt ttcatctttg
     2821 tcccacataa gtcataacat taacaataat aattgaatat agatttcttg tctaattgac
     2881 tgcccttaat aagacattgc ttcattgacc agttaagatt aaacataaca accagaaggg
     2941 aacaaaaagg ctcaaatagg catgaaatta caagcacctt gcttgaccta attacagtga
     3001 cgagcctcat ctcaccagat gccccaatct gacggtgggg gttcaagtct cctcatctat
     3061 ggaggtcact gtttacacaa tatgcctcac ttttctgaat gacctcattg gtcaatatgc
     3121 cagttctgtg agaatcgatc aaaaatagct ttaaagatta aagattttaa gttttcttct
     3181 cactttttta ggtagattat gtgttgaaat gaacacaaca gtttataccc atatctcaga
     3241 gatcatatcg atgatgaact ttaatgaatg ataagttatt atattatata ttaaatcata
     3301 ttatattata atatattata ttctatattc gttttgatag atgtagtgtt taaatgaaca
     3361 gtttatactc atctcaagga tcataaagaa gtgtcatatc atagaactgt aaatgacaat
     3421 gttatattat tttgatggac ctttagatat attctttggt tattatactg tatatttttg
     3481 gtagattatg tttacatcat agagctttaa atgactagtt ctattctagt ctatttgatt
     3541 tttttttttt taaatgacca caacagttta tactcaaata tcagtgatca taatgttata
     3601 tcaacatata aaatctatat tatgttatat tatattatat tatattatat tatattatat
     3661 tatattatat tttattttat attagacagc acccattctc agttttacat ctattatttg
     3721 actgtcagct tgacttaagt aggagtttga tcattagtcg tcactttcct cgagttcaga
     3781 ggattacgca ttcgattttt aagaccattt tctccttcgg aatgaacggc gctttatgct
     3841 ttaaatgaaa accagattta ctaaatcctg gattaagctg ttaaactgat aatagacctt
     3901 ctcactttcc cctcgtcaag accgagtaaa tctttaatct gctcaacact gtctcaaaaa
     3961 ttacaggagt agcttgtaac atatggcctt ttgcatagga gtccagacaa gcctatcatg
     4021 tgaacgagtg aaaaaacaag gccgtttaac atgtctggac caagcccttt aaacgggaca
     4081 agcagcagct ggtcagggat gccaatctgc aatcaatgaa aacaaactaa accagtggat
     4141 tgggtttata tgcccaattg aacagcagaa ttactcatct aatgcagatg agcgtatatg
     4201 gcttgatggc cacagtcagc cgggacatga attatgcaga agagatatag cgatttgtca
     4261 cttgtacaca aatcagatgt aataatgtat gtatttagtg cagtatttta tatagaacgc
     4321 tactttacga tttcaagtac ctgtaggaaa atatgttata gataattact atactataga
     4381 ggtgataatt cataatgcat aactttaaaa acattttgtt tcatagtata gcaattatat
     4441 atacagtata taagatagac tgcatatata tatatatata tatatattat atctgtgtgt
     4501 tattatatta taaccttagt ataacaataa tagtattttg gaaaggttta tgaatgagtt
     4561 tttttgccaa caggcttttt ttggaggggg tgaaactgtg actgtctgaa tctgctttgt
     4621 ttgattcaca ttgaacagat tgagatcgac agcttgtcga agacttctgt ctgtctcaat
     4681 tcctctgaga cactcaaatc aattatttag tgctgcccca caaatgtgtt ttatatggtt
     4741 ttgttattgg tttactatat attattaaat atatagaatg taatatgtaa tatagagaaa
     4801 tgtgctgatg atacagtaga tgaatactga cactatatta aaatattcta tataaaagta
     4861 taaaatttga acactgacgc tatattaaaa gattgcaaat gaccgtataa atgtttaaaa
     4921 tgtagaaact tcacatgtta ttttttctaa acaagcatgc tggttattac tttataatta
     4981 tgtaacaata taactgtact aatacagttc agtaattaac aagtaattca aaaatatttt
     5041 tattttaaat gtttatattt tatcattatt cttcaagcca ctaggttata ttagtttgtc
     5101 agcaaaaatg aacaaattag caccaacgct tataatttaa aatattaaat cttgaaaata
     5161 ttttaaagaa tgttacattt aatgaaaatt attttaaaaa gcataagaaa aattacacac
     5221 tcaaattgca tttaatatgc tttccgtgta aattacttat gtaaatttaa tttgatgcac
     5281 actaaaatgt ctcgtctatg acctatatac ataatgtatt atcatattgt aactcaaata
     5341 ttacatgtaa tattaatatt accttaaata tttatatatt acaatttaac attatgaata
     5401 acaaagacaa ctggagtatg tttgaccaaa acaaaacaac aatctttcta tttgtaaaag
     5461 ttaattttaa aaaaactgtt gcgatgtgta aaatatccat ttactttatt cagtaaaact
     5521 aatgtggatt ataatagtat agcataaata tataacaata tatcaattta atattaacat
     5581 caatataaaa ttgatgaatc tatctatgaa aaaatatgaa aaattcatta tttactcact
     5641 tattactaat tgcacacccg tatactattc ttttttcatt gaacacaaag aaagatatgt
     5701 tgaagtatgc tgaaaaccgg taaccattga cttcaacagt atttattttt ccaatggcag
     5761 ccagtggtta caggtttgca gctttcaacc aaatattttc ttttgtgatc agcagaagaa
     5821 agaaactcat aaaagtttgg aaccacttaa ataaaatagt gcataaatag tctgtaaatg
     5881 ttcatttttg tatgaactat cccttcaaat tatcgtttat attaaaagat tccagtaata
     5941 aatgtaaata atgagaccta caaccctgta aaacaaattg caaaatcaaa ctgactctat
     6001 acaggcaatt actcctgcaa acaatgttct catttaagaa atccaaagat gtcacggttg
     6061 cttaaagatg catgcacttt ttaattaggt aatggtcctt gccacccggc gctggttgtg
     6121 gccagatggt aagttatctc tggtttgtaa cgcctgtttt cagtgataga gcagccaaag
     6181 ttaattggaa tgtcattaaa gtttgagtta ctgggggttg gggctggact gcactagtcc
     6241 tgctgttgga gatgtgcgtt tcattaataa taatggatgt ttggatggag taataagggt
     6301 ggggctccac ctgctgccac ctgttaatac tctatataag catcgctcat ccttatggca
     6361 gaacagaagc gattaagaac catccggggg ggatccacta gttctagagc ggccgccacc
     6421 gcggtggagc tccagctttt gttcccttta gtgagggtta attcaagttt gtacaaaaaa
     6481 gcaggctgga ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     6541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     6601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     6721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     6781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     6841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     6901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     6961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     7021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     7081 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     7141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     7201 ctgtacaagg gaggaggaag atctaagctg aaccctcctg atgagagtgg ccccggctgc
     7261 atgagctgca agtgtgtgct ctcctgagac ccagctttct tgtacaaagt tgatgtagat
     7321 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7381 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7441 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     7501 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggccctcga gcatatgaga
     7561 tctgtcccga tctttctctt ctgtgctgtc aatttagagt agtttttgag gtaatttggg
     7621 tgcattctct gtaaaaacaa caaataaata tttatattgt atgcaataaa tgtaacaatt
     7681 acacatacac tctctgatta cagcattaaa gggttagttc acccaaaaat gaaaagatgt
     7741 cattaatgac tcaccctcat gtcgttccaa gcccgtaaca cctccgttca tcttcggaac
     7801 acagtttaag atattttaga tttagtccga gagctttctg tccctccact gaaaatgtat
     7861 gtacggtata ctgtccatgt ccagaaaggt aataaaaaca tcaaagtagt ccatgtgaca
     7921 tcagtgggtt aggtagaatt atttgaagca tcgaaaatac attttggtcc aaaaataaca
     7981 aaacctacga ctttattcag cattgtagcg tcactgcgaa gtcgtgaacg cggattgaca
     8041 acagacccgg aagagaatac aatgccgaat aaagtcgtag gttttgttat ttttggacca
     8101 aaatgtattc gatgcttcaa aaaattctaa ctaacccact gatgtcacat ggactacttt
     8161 gatgttttta ttacctttct ggacatggac agtataccgt acatacattc tcaatggagg
     8221 cacagaaagc tctcggacta aatctaaaat atcttaaact gtgttctgaa gatgaacgga
     8281 ggtcttacgg gcttggaacg acatgagggc gagtcattaa tgacattatt ttcatttttg
     8341 ggtgaactaa ccctttaata aaactgacat ttgcagccaa ttgcacactt tataatgtta
     8401 aatacttgtt gattttagaa atacagtagc atttagtgac aatcaaacat taattttatt
     8461 tctgattata ttgacaaaaa cacaggactg taaggctgac atgacagggt gaattgattt
     8521 cattacatta gttttgatgg taacactttt acaataaggt tcgttagtta actacattag
     8581 ttaacatgaa ctaataatga actgcacgta tacatgttaa tttcaacatt tactaatact
     8641 ttatatcttg ttaacattag ttaatgcact gtgaactaac atgaacaaac aatgaacatc
     8701 tgtatttcta ttaactaacg ttaacaaaga ttaacagata cagtaacaaa tgtattgctc
     8761 atggttattt aatgttagtt aatacatgaa ctaatcaacc tcattgtaaa gtgttaccgt
     8821 tttgacaata caacatgttt ggaaaaaaat ttctcgcttg gttttcttgt gttttgcatg
     8881 aattcaaaca caatgagagc acggaatgat aatgattatt caaagctgca ataggcgcca
     8941 aatttcccgt ggacctaacg ttaccaatta caaatgcatt ttagcatttt attacgtgag
     9001 ccttatattg tcaatcgcat gcattagtga aaataggcct actacacata taaaaggcgc
     9061 gccatcaacg tcaaaacatg cacacccaaa aaaaaaaaaa aaaaaaaaaa aaaaaactat
     9121 cagtaaaaca aaatacttaa tgtacttacc tcaatatgct tccttaggtt tgatggcgaa
     9181 cttttgaagg ccgatatttc tttattaagc gggagacaga ggacacattt catcttgaat
     9241 gaatctttat ttacaccact taaagaaaag aattcgcgaa gatacggcca cgggtgctct
     9301 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
     9361 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
     9421 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
     9481 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
     9541 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
     9601 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     9661 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     9721 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     9781 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
     9841 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     9901 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9961 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    10021 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10081 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    10141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    10201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10501 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10621 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10681 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10741 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10801 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10861 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10921 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10981 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11041 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11101 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11161 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11221 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11281 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11341 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11401 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11461 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11521 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11581 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11641 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11701 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11761 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11821 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11881 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11941 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12001 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12061 gtg
//
