LOCUS       sequence-222826-        7912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Jung CH, Jun CB, Ro SH, Kim YM, Otto NM, Cao J, Kundu M, Kim DH
  TITLE     ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy 
            machinery.
  JOURNAL   Mol Biol Cell. 2009 Apr;20(7):1992-2003. Epub 2009 Feb 18.
  PUBMED    19225151
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2009-04"; volume: "20(7)"; pages: "1992-2003. Epub 
            2009 Feb 18"
COMMENT     Sequence Label: myc-hULK1
FEATURES             Location/Qualifiers
     source          1..7912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        250..629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        630..833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     784..804
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     830..854
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1064..1082
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1064..1081
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1095..1114
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1199..1208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1208..1237
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      4448..4457
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4461..4595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4498..4517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4552..4571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4664..5021
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4664..4855
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4687..4707)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4872..5007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5041..5058)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5041..5057)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5050..5072)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5270..5725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5357..5376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5567..5588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5742..5761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5861..5883
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5921..5939)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6007..6111
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6112..6180,6181..6972)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6330..6349)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7143..7731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7632..7651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7885..7902
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg
      241 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      841 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      901 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
      961 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac
     1021 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
     1081 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct
     1141 cggttctatc gattgaattc ccgaaccgac agtcggtctc ttcaccaagg ccattcgcgc
     1201 caccatggag caaaagctca tttctgagga agatctcaat ggtggtggtg gtgggtcgac
     1261 catggagccc ggccgcggcg gcacagagac cgtgggcaag ttcgagttct cccgcaagga
     1321 cctgatcggc cacggcgcct tcgcggtggt cttcaagggc cgccaccgcg agaagcacga
     1381 tttggaggtc gccgtcaagt gcattaacaa gaagaacctc gccaagtctc agacgctgct
     1441 ggggaaggaa atcaaaatcc tgaaggaact gaaacatgaa aacatcgtgg ccctgtacga
     1501 cttccaggaa atggctaatt ctgtctacct ggttatggag tactgcaacg gtggggacct
     1561 ggccgactac ctgcacgcca tgcgcacgct gagcgaggac accatcaggc tcttcctgca
     1621 gcagatcgcg ggcgccatgc ggcttctgca cagcaaaggc atcatccacc gcgacctgaa
     1681 accgcagaac atcctgctgt ccaaccccgc cggccgccgc gccaacccca acagcatccg
     1741 cgtcaagatc gctgacttcg gcttcgcgcg gtacctccag agcaacatga tggcggccac
     1801 actctgcggc tcccccatgt acatggcccc cgaggtcatc atgtcccagc actacgacgg
     1861 gaaggcggac ctgtggagca tcggcaccat cgtctaccag tgcctgacgg ggaaggcgcc
     1921 cttccaggcc agcagccccc aggacctgcg cctgttctac gagaagaaca agacgttggt
     1981 ccccaccatc ccccgggaga cctcggcccc gctgcggcag ctgctcctgg ccctactgca
     2041 acgcaaccac aaggaccgca tggacttcga tgagtttttt catcaccctt tcctcgatgc
     2101 cagcccctcg gtcaggaaat ccccacccgt gcctgtgccc tcgtacccaa gctcggggtc
     2161 cggcagcagc tccagcagca gctccacctc ccacctggcc tccccgccgt ccctgggcga
     2221 gatgcagcag ctgcagaaga ccctggcctc cccggctgac accgctggct tcctgcacag
     2281 ctcccgggac tctggtggca gcaaggactc ttcctgtgac acagacgact tcgtcatggt
     2341 ccccgcgcag tttccaggtg acctggtggc tgaggcgccc agtgccaaac ccccgccaga
     2401 cagcctgatg tgcagtggga gctcactggt ggcctctgcg ggcttggaga gccacggccg
     2461 gaccccatct ccatccccac cctgcagcag ctcccccagt ccctcaggcc gggctggccc
     2521 gttctccagc agcaggtgcg gcgcctctgt ccccatccca gtccccacgc aggtgcagaa
     2581 ctaccagcgc attgagcgaa acctgcagtc acccacccag ttccaaacac ctcggtcctc
     2641 tgccatccgc aggtcaggca gcaccagccc cctgggcttt gcaagggcca gcccctcgcc
     2701 ccctgcccac gctgagcatg gaggcgtcct ggccaggaag atgtctctgg gtggaggccg
     2761 gccctacacg ccatctcctc aagttggaac catccctgag cggccaggct ggagcgggac
     2821 gccctcccca cagggagctg agatgcgggg tggcaggtcc cctcgtccag gctcctctgc
     2881 acccgagcac tctccccgca cttccgggct gggctgccgc ctgcacagcg cccccaacct
     2941 gtctgacttg cacgtcgtcc gccccaagct gcccaaaccc cccacggacc ccctgggagc
     3001 tgtgttcagc ccaccacagg ccagccctcc ccagccgtcc cacggcctgc agtcctgccg
     3061 gaacctgcgg ggctcaccca agctgcccga cttcctgcag cgaaaccccc tgccccccat
     3121 cctgggctcc cccaccaagg ctgtgccctc ctttgacttc ccgaagaccc ccagctccca
     3181 gaacctgctg gccctcctag cccggcaggg cgtggtgatg acgccccctc gaaaccggac
     3241 gctgcccgac ctctcggagg tgggaccctt ccatggtcag ccgttgggcc ctggcctgcg
     3301 gccaggcgag gaccccaagg gcccctttgg ccggtctttc agcaccagcc gcctcactga
     3361 cctgctcctt aaggcggcgt ttgggacaca agccccggac ccgggcagca cggagagcct
     3421 gcaggagaag cccatggaga tcgcaccctc agctggcttt ggagggagcc tgcacccagg
     3481 agcccgtgct gggggcacca gcagcccctc cccggtggtc ttcaccgtgg gctctccccc
     3541 gagcgggagc acgccccccc agggcccccg caccaggatg ttctcagcgg gccccactgg
     3601 ctctgccagc tcttctgccc gccacctggt gcctgggccc tgcagcgagg ccccagcccc
     3661 tgagctccct gctccaggac acggctgcag ctttgccgac cccattgctg cgaacctgga
     3721 gggggctgtg accttcgagg cccccgacct ccctgaggag accctcatgg agcaagagca
     3781 cacggagatc ctgcgtggcc tgcgcttcac gctgctgttc gtgcagcacg tcctggagat
     3841 cgcagccctg aagggcagcg ccagtgaggc ggcggggggc cctgagtacc agctgcagga
     3901 gagtgtggtg gccgaccaga tcagcctgct gagccgagaa tggggcttcg cggaacagct
     3961 ggtgctgtac ctgaaggtgg ccgagctact gtcctccggc ctgcaaagtg ccatcgacca
     4021 gatccgggcc ggcaagctct gcctgtcgtc cactgtgaag caggtggtgc gcaggctgaa
     4081 tgagctgtac aaggccagcg tggtgtcctg ccagggcctg agcctgcggc tgcagcgctt
     4141 cttcctggac aagcagcggc tcctggaccg cattcacagc atcactgccg agaggctcat
     4201 cttcagccac gctgtgcaga tggtgcagtc ggctgccctg gacgagatgt tccagcaccg
     4261 tgagggctgc gtcccacgct accacaaggc cctgctgctc ctggaggggc tgcagcacat
     4321 gctctcggac caggccgaca tcgagaacgt caccaagtgc aagctgtgca ttgagcggag
     4381 actctcggcg ctgctgactg gcatctgtgc ctgagcggcc gctaagtaag taaggatccc
     4441 cagcttggcc gccatggccc aacttgttta ttgcagctta taatggttac aaataaagca
     4501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4561 ccaaactcat caatgtatct tatcatgtct ggatcgggaa ttaattcggc gcagcaccat
     4621 ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc
     4681 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4741 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4801 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4861 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4921 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4981 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctgtta acagcttggc
     5041 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5101 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5161 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     5221 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     5281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5461 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     5521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5581 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     5641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5701 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     5761 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5821 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5881 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5941 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6001 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6061 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6121 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6181 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6241 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6301 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6361 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6421 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6481 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6541 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6601 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6661 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6721 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6781 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6841 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6901 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6961 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7021 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7141 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7201 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7261 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7321 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7381 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7441 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7501 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7561 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7621 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7681 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7741 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7801 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7861 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
