LOCUS       sequence-222847-        9883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with 
            RCH1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou 
            W, Kulikova O, Hirt H, Bisseling T
  TITLE     Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 
            2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell 
            Number.
  JOURNAL   Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. 
            Epub 2017 Aug 30.
  PUBMED    28855334
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00366"; journalName: "Plant Cell"; date: "2017-09"; 
            volume: "29"; issue: "9"; pages: "2183-2196"
COMMENT     Sequence Label: RCH1:HDT1,HDT2-RNAi
FEATURES             Location/Qualifiers
     source          1..9883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(297..321)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(725..1384)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1299..1318
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    1760..1784
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3021..3120)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3136..3153)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3136..3152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3145..3167)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3216..3302
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3394..3421
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3580..3597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3751..4339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3831..3850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4585..5376)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(6239..6418)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      8378..8630
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8912..8931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9122..9143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    9569..9593
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcctgaagct gttcctgctc ctgcccctac tgctgttact gccaacggaa atgctggagc
       61 agctgttgtc aaggctgaca caaagccaaa ggccaaacct gccgaagtga agcctgcaga
      121 agagaagcct gaatcagacg aggaagatga gtctgatgat gaagatgagt ctgaagagga
      181 tgatgactct gagaaaggaa tggatgttga tgaagatgac tcagatgatg acgaggagga
      241 ggattctgag gatgaagaag aggaggagac tcctaagaag aagggtgggc gcgccgaccc
      301 agctttcttg tacaaagtgg tgatatcccg cgggatcaga agcaacctca tggaaatgat
      361 gaggtaaggt ttcatactct tgcctcttct tacggctttc tgtgtcttca ctgtaagttt
      421 ctatgatttg agccaccaat atatatgctc tggtgtgctg agttatgttt atctggtcac
      481 gcttagtggg taaaattatg cttattttag cataaacttt aatgagatta ggttttgtat
      541 cacaccgatc tttagttgtt tagtaagatg acagaaattc ttggtaaaac actctaaatc
      601 gtcttcttta gtgaagtttt ccttagagta gcataaattt tggctttttt cttgatggtt
      661 gaataaggtg gcacttgttg gtatgagact ttattgagag tcatattaag ctggatccac
      721 gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      781 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      841 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      901 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
      961 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1021 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1081 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1141 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     1201 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1261 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1321 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1381 ccattttagc ttccttagct cctgaaaatc tcgacggatc cagcttagcg ttcattgaat
     1441 ttgatggcca taggggttta gatgcaactg tttctttgaa cattgtagaa atatataaag
     1501 attttacatt agcctactct tgaaagtcaa attgtcgaat ttgattatat tatactctag
     1561 aggtgatatt agttaatgag tttatactcg gttatttaca gcttattcat ataccagtta
     1621 acgtgtctca tatattctaa cttcttagca tttaacgtgt ttgcaggtca gcttgacact
     1681 gtacataaca gcatcactag tgcggccgcc tgcaggtcga ccatatggtc gacctgcagg
     1741 cggccgcact agtgatatca ccactttgta caagaaagct gggtcggcgc gcccaccctt
     1801 cttcttagga gtctcctcct cttcttcatc ctcagaatcc tcctcctcgt catcatctga
     1861 gtcatcttca tcaacatcca ttcctttctc agagtcatca tcctcttcag actcatcttc
     1921 atcatcagac tcatcttcct cgtctgattc aggcttctct tctgcaggct tcacttcggc
     1981 aggtttggcc tttggctttg tgtcagcctt gacaacagct gctccagcat ttccgttggc
     2041 agtaacagca gtaggggcag gagcaggaac agcttcagga acatcctcat tatccgaact
     2101 agtgaagtca tcctgctcga tgttggggga tttgtagcca atgaaatgaa catttgcttt
     2161 ggtaccgctg tgtgaaagct caaactcttt atcaaaaacc aaatcaaagc taatctgagg
     2221 gaacttgtct tgtgaaagtg ttccaataac aagtttagcc ccaccaacag tcacactcaa
     2281 aaccacagat tctccagatt tcactgtgca gtcaagtgaa gcctgagaaa tgtggacaag
     2341 gctgtcttct tcaggagtca ccttagtagc gttttttggt gtcaccgcaa ttccccagaa
     2401 ctcatctgtt ttctgaggag taactgctac tttcgccttc tttgctgaca caggtgcttt
     2461 aggggtagtt tcattagctc tcttcttgct tgatgcaggc ttcttaggtg taggctcttc
     2521 ttcctcagaa tcctcaccat cagaatcatc ttcatccatt ccgtcagaat cagactcatc
     2581 ctcttcatca tcaacagctg gcttcacttc tgcaggctta gcctttggtt tagctacagc
     2641 cttggcagca ttcccagcag gaacttcttc ctcttcttcc tcttcttcct cagaatagcc
     2701 ttgtggctca atgttgggag ttttgtatcc aacaaagtaa acacttcctt ttccccaagt
     2761 gtgagaaagc tcaaactcct tgtcgaatac caaatcacag aaaagctgag ggatgttctc
     2821 agtcgataga gttcccagaa ccaagttctg gttcccaacc tttacatgta aaggcacaaa
     2881 ctctcccttc ttgtttttac attctccaag cgatgcctga gaaacgtgga taagaatgcc
     2941 ttcttcagga gtcactgtaa ctggctttcc tgatttaact tcaattcccc agaactccat
     3001 ggtgaagggg gcggccgcgg agcctgcttt tttgtacaaa gttggcatta taaaaaagca
     3061 ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     3121 gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc
     3181 tagcgttaac gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg
     3241 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     3301 atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac
     3361 gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt
     3421 ttgcgtttct acaaactctt cctggctagc ggtacgcgta ttaattgcgt tgcgctcact
     3481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     4201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4381 gaacgaaaac tcacgttaag ggattttggt catgcatgat atatctccca atttgtgtag
     4441 ggcttattat gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga
     4501 gcaattatgt gcttagtgca tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg
     4561 cttgaacgaa tttctagcta gacattattt gccgactacc ttggtgatct cgcctttcac
     4621 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     4681 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     4741 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     4801 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     4861 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     4921 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     4981 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     5041 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     5101 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     5161 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     5221 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     5281 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     5341 ttgagtcgat acttcggcga tcaccgcttc ccccatgatg tttaactttg ttttagggcg
     5401 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     5461 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca
     5521 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     5581 gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca
     5641 ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta caatcaacat
     5701 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
     5761 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
     5821 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgg gtgggtgtag
     5881 agcgtsragc ccagtcccgt ccgctggtgg cggggggmga cgtacacggt cgactcggcc
     5941 gtccagtcgt aggcgttgcg tgccttccag ggacccgcgt aggcgatgcc ggcgacctcg
     6001 ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc
     6061 cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg
     6121 ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg
     6181 cgtcgttctg ggctcatggt agatcccctc gatcgagttg agagtgaata tgagactcta
     6241 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     6301 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     6361 cttagctcat taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg
     6421 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     6481 cttaattctc cgctcatgat cgataattcg cggtaccgag ctcgaattcg attcggagct
     6541 ctcttcatcg gtgattgatt cctttaaaga cttatgtttc ttatcttgct tctgaggcaa
     6601 gtattcagtt accagttacc acttatattc tggactttct gactgcatcc tcatttttcc
     6661 aacattttaa atttcactat tggctgaatg cttcttcttt gaggaagaaa caattcagat
     6721 ggcagaaatg tatcaaccaa tgcatatata caaatgtacc tcttgttctc aaaacatcta
     6781 tcggatggtt ccatttgctt tgtcatccaa ttagtgacta ctttatatta ttcactcctc
     6841 tttattacta ttttcatgcg aggttgccat gtacattata tttgtaagga ttgacgctat
     6901 tgagcgtttt tcttcaattt tctttatttt agacatgggt atgaaatgtg tgttagagtt
     6961 gggttgaatg agatatacgt tcaagtgaag tggcataccg ttgtcgagta aggatgacct
     7021 acccattctt gagacaaatg ttacatttta gtatcagagt aaaatgtgta cctataactc
     7081 aaattcgatt gacatgtatc cattcaacat aaaattaaac cagcctgcac ctgcatccac
     7141 atttcaagta ttttcaaacc gttcggctcc tatccaccgg gtgtaacaag acggattccg
     7201 aatttggaag attttgactc aaattcccaa tttatattga ccgtgactaa atcaacttta
     7261 acttctataa ttctgattaa gctcccaatt tatattccca acggcactac ctccaaaatt
     7321 tatagactct catccccttt taaaccaact tagtaaacgc tctttttttt aattttatga
     7381 agttaagttt ttaccttgtt tttaaaaaga atcgttcata agatgccatg ccagaacatt
     7441 agctacacgt tacacatagc atgcagccgc ggagaattgt ttttcttcgc cacttgtcac
     7501 tcccttcaaa cacctaagag cttctctctc acagcacaca catacaatca catgcgtgca
     7561 tgcattatta cacgtgatcg ccatgcaaat ctcctttata gtctataaat taactcatcc
     7621 gcttcactct ttactcaaac caaaactcat caatacaaac aagattaaaa acatacccca
     7681 tggggtcttc caagaatgtt atcaaggagt tcatgaggtt taaggttcgc atggaaggaa
     7741 cggtcaatgg gcacgagttt gaaatagaag gcgaaggaga ggggaggcca tacgaaggcc
     7801 acaataccgt aaagcttaag gtaaccaagg ggggaccttt gccatttgct tgggatattt
     7861 tgtcaccaca atttcagtat ggaagcaagg tatatgtcaa gcaccctgcc gacataccag
     7921 actataaaaa gctgtcattt cctgaaggat ttaaatggga aagggtcatg aactttgaag
     7981 acggtggcgt cgttactgta acccaggatt ccagtttgca ggatggctgt ttcatctaca
     8041 aggtcaagtt cattggcgtg aactttcctt ccgatggacc tgttatgcaa aagaagacaa
     8101 tgggctggga agccagcact gagcgtttgt atcctcgtga tggcgtgttg aaaggagaga
     8161 ttcataaggc tctgaagctg aaagacggtg gtcattacct agttgaattc aaaagtattt
     8221 acatggcaaa gaagcctgtg cagctaccag ggtactacta tgttgactcc aaactggata
     8281 taacaagcca caacgaagac tatacaatcg ttgagcagta tgaaagaacc gagggacgcc
     8341 accatctgtt ccttatgtac aagtaaagcg gatccccgat cgttcaaaca tttggcaata
     8401 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     8461 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     8521 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     8581 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggcctcctg
     8641 tcaatgctgg cggcggctct ggtggtggtt ctggtggcgg ctctgagggt ggtggctctg
     8701 agggtggcgg ttctgagggt ggcggctctg agggaggcgg ttccggtggt ggctctggtt
     8761 ccggtgattt tgattatgaa aagatggcaa acgctaataa gggggctatg accgaaaatg
     8821 ccgatgaaaa cgcgctacag tctgacgcta aaggcaaact tgattctgtc gctactgatt
     8881 acggtgctgc tatcggcctg aatggcgccc gctcctttcg ctttcttccc ttcctttctc
     8941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     9061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     9181 ttataaggga ttttgccgat ttcggaacca ccatcaaaca ggattttcgc ctgctggggc
     9241 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     9301 gcgccatcga tgggcccgac gtcgcatgcc tgcaggtcac tggattttgg ttttaggaat
     9361 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     9421 tgctcaacac atgagcgaaa ccctataaga accctaattc ccttatctgg gaactactca
     9481 cacattattc tggagaaaaa tagagagaga tagatttgta gagagagact ggtgattttt
     9541 gcggactcta gcatggccgc gggatatcac aagtttgtac aaaaaagcag gctccgcggc
     9601 cgcccccttc accatggagt tctggggaat tgaagttaaa tcaggaaagc cagttacagt
     9661 gactcctgaa gaaggcattc ttatccacgt ttctcaggca tcgcttggag aatgtaaaaa
     9721 caagaaggga gagtttgtgc ctttacatgt aaaggttggg aaccagaact tggttctggg
     9781 aactctatcg actgagaaca tccctcagct tttctgtgat ttggtattcg acaaggagtt
     9841 tgagctttct cacacttggg gaaaaggaag tgtttacttt gtt
//
