LOCUS       sequence-222857-       10110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chloroplast-targeted expression of mCitrine controlled by the CaMV 
            35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/CTP-mCitrine
FEATURES             Location/Qualifiers
     source          1..10110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        626..970
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     914..934
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1234..1236,1237..1950)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1276..1297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1537..1556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1884..1905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1953..1977)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2297..2321
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3621..4250
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4684..5751
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5817..6011
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6247..6269)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6355..6495
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6410..6429
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6510..6527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6681..7269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6761..6780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7513..8304)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8832..8856
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8949..9203)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9226..9798)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9840..10019)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ctcccatatg gtcgactaga gccaagctga tctcctttgc cccggagatc accatggacg
       61 actttctcta tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt
      121 tcaccaccga taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga
      181 tggttagaga ggcctacgcg gcaggtctca tcaagacgat ctacccgagt aataatctcc
      241 aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      301 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      361 cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag
      421 tagttcctac tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac
      481 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
      541 aaatcttcgt caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
      601 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
      661 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
      721 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
      781 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      841 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      901 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      961 tggagaggac tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt
     1021 acaatttact attctagtcg acctgcaggc ggccgcacta gtgatatcat ggcttcctct
     1081 atgctctctt ccgctactat ggttgcctct ccggctcagg ccactatggt cgctcctttc
     1141 aacggactta agtcctccgc tgccttccca gccacccgca aggctaacaa cgacattact
     1201 tccatcacaa gcaacggcgg aagagttaac tgcgtgagca agggcgagga gctgttcacc
     1261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
     1441 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1681 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1801 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa
     1861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1921 actctcggca tggacgagct gtacaagtag tcgttcagct ttcttgtaca aagtggtgat
     1981 atcccgcggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct
     2041 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta
     2101 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     2161 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctgcaggcat
     2221 gcgacgtcgg gcccaagctt agcttgagct tggatcagat tgtcgtttcc cgccttcagt
     2281 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2341 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2401 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     2461 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     2521 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     2581 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     2641 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     2701 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     2761 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     2821 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     2881 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     2941 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     3001 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     3061 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     3121 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     3181 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     3241 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     3301 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     3361 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     3421 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     3481 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     3541 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     3601 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     3661 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     3721 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     3781 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     3841 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     3901 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     3961 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     4021 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     4081 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     4141 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     4201 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     4261 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     4321 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     4381 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     4441 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     4501 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     4561 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     4621 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     4681 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     4741 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     4801 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     4861 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     4921 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     4981 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     5041 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     5101 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     5161 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     5221 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     5281 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     5341 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     5401 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     5461 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     5521 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     5581 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     5641 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     5701 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     5761 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     5821 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     5881 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     5941 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     6001 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     6061 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     6121 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     6181 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     6241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     6361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     6421 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6481 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7081 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7321 tcacgttaag ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat
     7381 gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt
     7441 gcttagtgca tctaatcgct tgagttaacg ccggcgaagc ggcgtcggct tgaacgaatt
     7501 tctagctaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     7561 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     7621 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     7681 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     7741 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     7801 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     7861 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     7921 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     7981 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     8041 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     8101 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     8161 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     8221 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     8281 ttcggcgatc accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg
     8341 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     8401 cttggatgcc cgaggcatag actgtacccc aaaaaaacat gtcataacaa gaagccatga
     8461 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     8521 acggcagtta cgctacttgc attacagctt acgaaccgaa cgaggcttat gtccactggg
     8581 ttcgtgcccg aattgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     8641 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     8701 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     8761 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     8821 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
     8881 aacacattgc ggacgttttt aatgtactga attaacgccg aattgaatta tcagcttgca
     8941 tgccggtcga tctagtaaca tatagatgac accgcgcgcg ataatttatc ctagtttgcg
     9001 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     9061 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     9121 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     9181 tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcattagg ggcagggcat
     9241 gctcatgtag agcgcctgct cgccgtccga ggcggtgccg tcgtacaggg cggtgtccag
     9301 gccgcagagg gtgaacccca tccgccggta cgcgtggatc gccggtgcgt tgacgttggt
     9361 gacctccagc cagaggtgcc cggcaccccg ctcgcgggcg aactccgtcg cgagccccat
     9421 caacgcgcgc ccgaccccgt gcccccggtg ctccggggcg acctcgatgt cctcgacggt
     9481 cagccggcgg ttccagccgg agtacgagac gaccacgaag cccgccaggt cgccgtcgtc
     9541 cccgtacgcg acgaacgtcc gagagtccgg gtcgccgtcc tccccgtcgt ccgattcgtc
     9601 gtccgattcg tcgtcgggga acaccttggt caggggcggg tccaccggca cctcccgcag
     9661 ggtgaagccg tccccggtgg cggtgacgcg gaagacggtg tcggtggtga aggacccatc
     9721 cagtgcctcg atggcctcgg cgtcccccgg gacactggtg cggtaccggt aagccgtgtc
     9781 gtcaagagtg gtacccatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
     9841 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     9901 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     9961 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
    10021 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
    10081 ccttaattct catgtatgat aattcgagct
//
