LOCUS       sequence-22289-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Tee AR, Manning BD, Roux PP, Cantley LC, Blenis J
  TITLE     Tuberous sclerosis complex gene products, Tuberin and Hamartin, 
            control mTOR signaling by acting as a GTPase-activating protein 
            complex toward Rheb.
  JOURNAL   Curr Biol. 2003 Aug 5. 13(15):1259-68.
  PUBMED    12906785
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Aug 5. 13(15):1259-68."
COMMENT     Sequence Label: pRK7-Rheb
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      648..657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(698..717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtccaactgc acctcggttc taagcttctg caggtcgact ctagaggatc caccatggac
       61 tataaggacg atgatgacaa accgcagtcc aagtcccgga agatcgcgat cctgggctac
      121 cggtctgtgg ggaaatcctc attgacgatt caatttgttg aaggccaatt tgtggactcc
      181 tacgatccaa ccatagaaaa cacttttaca aagttgatca cagtaaatgg acaagaatat
      241 catcttcaac ttgtagacac agccgggcaa gatgaatatt ctatctttcc tcagacatac
      301 tccatagata ttaatggcta tattcttgtg tattctgtta catcaatcaa aagttttgaa
      361 gtgattaaag ttatccatgg caaattgttg gatatggtgg ggaaagtaca aatacctatt
      421 atgttggttg ggaataagaa agacctgcat atggaaaggg tgatcagtta tgaagaaggg
      481 aaagctttgg cagaatcttg gaatgcagct tttttggaat cttctgctaa agaaaatcag
      541 actgctgtgg atgtttttcg aaggataatt ttggaggcag aaaaaatgga cggggcagct
      601 tcacaaggca agtcttcatg ctcggtgatg tgagaattca atcgatggcc gccatggccc
      661 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
      721 aataaagcat ttttttcact
//
