LOCUS       sequence-222908-        9609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PguCas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pC0067	PguCas13 (B11) His6-TwinStrep-SUMO-BsaI
FEATURES             Location/Qualifiers
     source          1..9609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(157..180,181..216,217..240)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             241..534
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(4110..4128)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4124..4171
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4346..4351,4352..4368,4369..4374))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4401..4419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4487..4591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4592..4660,4661..5452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4810..4829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5623..6211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6112..6131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6365..6382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6397..6536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6462..6481)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6622..6644
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6638..6829)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8141..9223)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9185..9204
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9224..9301)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9507..9526
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gctagctgga gccatccgca gtttgaaaaa
      181 ggtggtggta gcggtggtgg ttcaggtggt agtgcatggt cacaccctca gtttgagaaa
      241 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct
      301 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa
      361 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg
      421 gactccttaa gattcttgta cgacggtatt agaatccaag ctgatcagac ccctgaagat
      481 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggatctatg
      541 acagagcaga gcgagaggcc ctacaacggc acctactaca ccctggaaga taagcacttc
      601 tgggccgcct ttctgaacct ggccagacac aacgcctaca tcaccctgac acacatcgac
      661 cggcagctgg cctacagcaa ggccgacatc accaacgacc aggacgtgct gagcttcaag
      721 gccctgtgga agaacttcga caacgacctg gaaagaaaga gccggctgcg gagcctgatc
      781 ctgaagcact tcagctttct ggaaggcgcc gcttacggca agaagctgtt cgagtctaag
      841 agcagcggca acaagagcag caagaacaaa gagctgacca agaaagagaa agaggaactg
      901 caggccaacg ctctgagcct ggacaacctg aagtctatcc tgttcgactt cctgcagaag
      961 ctgaaggact tccggaacta ctacagccac taccggcaca gcggaagcag cgagctgcct
     1021 ctgttcgacg gcaacatgct gcagcggctg tacaacgtgt tcgacgtgtc cgtgcagcgc
     1081 gtgaagatcg accacgagca caacgatgag gtggaccctc actaccactt caaccacctc
     1141 gtgcggaagg gcaagaagga cagatacggc cacaacgaca accccagctt caagcaccac
     1201 ttcgtggacg gcgaaggcat ggttacagaa gccggcctgc tgttcttcgt gtccctgttc
     1261 ctggaaaagc gggacgccat ctggatgcag aagaagatcc ggggcttcaa aggcggcacc
     1321 gagacatacc agcagatgac caacgaggtg ttctgccggt ccagaatcag cctgcctaag
     1381 ctgaagctgg aaagcctgcg gatggacgac tggatgctgc tggacatgct gaacgaactc
     1441 gtgcggtgcc ccaagcctct gtacgacaga ctgagagagg acgaccgggc ctgctttaga
     1501 gtgcccgtgg atatcctgcc tgacgaggac gatacagatg gcggcggaga ggaccccttc
     1561 aagaataccc tcgtccggca ccaggacaga ttcccttact tcgccctgcg gtacttcgac
     1621 ctgaagaagg tgttcaccag cctgcggttc cacatcgatc tgggcaccta ccactttgcc
     1681 atctacaaga agatgatcgg cgagcagccc gaggacagac acctgaccag aaacctgtac
     1741 ggcttcggcc ggatccagga cttcgccgaa gaacacagac ccgaggaatg gaagcggctc
     1801 gtcagagatc tggactactt cgagacaggc gacaagccct acatcagcca gacaagccca
     1861 cactaccaca tcgagaaggg aaagatcggc ctgagattca tgcccgaggg ccagcatctt
     1921 tggccctctc cagaagtggg caccaccaga accggcagat ctaagtacgc ccaggacaag
     1981 agactgaccg ccgaggcctt tctgtctgtg cacgagctga tgcctatgat gttctactac
     2041 ttcctgctgc gcgagaagta cagcgaggaa gtgtctgccg agagagtgca gggcagaatc
     2101 aagcgcgtga tcgaggatgt gtacgccgtg tacgatgcct ttgccaggga cgagatcaac
     2161 acccgggatg agctggatgc ctgcctggcc gataagggca tcagaagagg ccatctgcct
     2221 cgccagatga tcgccatcct gagccaagag cacaaggaca tggaagagaa gattcggaag
     2281 aaactgcaag agatgatggc cgacaccgac caccggctgg atatgctgga tagacagacc
     2341 gaccggaaga tcagaatcgg ccggaagaat gccggactgc ctaagagcgg agtgatcgcc
     2401 gattggctcg tgcgggacat gatgagattt cagcccgtgg ccaaggacgc cagcggaaag
     2461 cctctgaaca actccaaggc caacagcacc gagtaccgga tgctgcagag agccctggct
     2521 ctgtttggag gcgagaaaga gaggctgacc ccatacttcc ggcagatgaa tctgaccggc
     2581 ggaaacaacc ctcatccttt tctgcacgaa actcgctggg agagccacac caacatcctg
     2641 tccttttacc ggtcctacct gagagcccgc aaggccttcc tcgaaaggat cggcagatcc
     2701 gacagagtgg aaaaccggcc atttctgctg ctgaaagagc ccaagaccga cagacagaca
     2761 ctggtggctg gatggaaggg cgagttccat ctgccaagag gcatcttcac agaggccgtg
     2821 cgggattgcc tgatcgagat gggacacgat gaagtggcca gctacaaaga agtgggattc
     2881 atggccaagg ccgtgccact gtactttgag agagcctgcg aggacagggt gcagcccttt
     2941 tacgacagcc ccttcaacgt gggcaacagc ctgaagccta agaagggcag attcctgagc
     3001 aaagaggaac gggccgaaga gtgggagcgg ggcaaagaga gattccggga tctcgaagcc
     3061 tggtcctata gcgccgccag aagaatcgag gacgcctttg ccggcatcga gtatgctagc
     3121 cccggaaaca agaagaaaat cgagcagctc ctgcgggacc tgagcctgtg ggaagccttt
     3181 gagagcaagc tgaaagtgcg ggccgaccgg atcaatctgg ccaagctgaa gaaagaaatc
     3241 ctggaagccc aagaacaccc ctaccacgac ttcaagagct ggcagaagtt cgagcgcgag
     3301 ctgcggctgg tcaagaacca ggatatcatc acctggatga tgtgccgcga cctgatggaa
     3361 gaaaacaagg tggaaggcct ggacaccggc acactgtacc tgaaggacat cagacccaac
     3421 gtgcaagagc agggcagcct gaacgtgctg aacagagtga agcccatgag actgcccgtg
     3481 gtggtgtaca gagccgatag cagaggccac gtgcacaaag aagaagcccc tctggccacc
     3541 gtgtacatcg aggaaagaga caccaagctg ctgaagcagg gcaacttcaa gtccttcgtg
     3601 aaggacaggc ggctgaacgg cctgttctcc ttcgttgata ctggcggact ggccatggaa
     3661 cagtacccca tctccaagct gagagtggaa tacgagctgg ccaagtacca gaccgccaga
     3721 gtgtgtgtgt tcgagctgac cctgagactg gaagagtccc tgctgacccg gtatcctcat
     3781 ctgcccgacg agagcttcag agagatgctg gaatcttgga gcgaccctct gctggccaaa
     3841 tggcctgaac tgcacggaaa agtgcggctg ctgatcgccg tgcggaatgc ctttagccac
     3901 aatcagtacc ctatgtacga cgaggccgtg ttcagctcca tccggaagta cgatcctagc
     3961 agccccgacg ccatcgaaga gagaatgggc ctgaatatcg cccaccgcct gtctgaggaa
     4021 gtgaagcagg ccaaagaaac cgtggaacgg atcattcagg cctaaggccg cactcgaggc
     4081 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     4141 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
     4201 cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg
     4261 gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag
     4321 caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga
     4381 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4441 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4501 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4561 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4621 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4681 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4741 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4801 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     4861 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4921 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4981 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5041 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5101 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     5161 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5221 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5281 tgataaatct ggagccggtg agcgtgggtc acgcggtatc attgcagcac tggggccaga
     5341 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5401 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5461 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5521 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5581 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5761 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5821 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5881 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5941 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6001 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6061 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6361 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     6421 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc
     6481 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     6541 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6601 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6661 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     6721 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     6781 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     6841 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     6901 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     6961 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     7021 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     7081 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     7141 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     7201 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     7261 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     7321 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     7381 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     7441 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     7501 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     7561 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     7621 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     7681 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     7741 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     7801 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     7861 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     7921 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     7981 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     8041 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     8101 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8221 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     8281 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     8341 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     8401 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     8461 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     8521 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     8581 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     8641 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     8701 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     8761 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     8821 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     8881 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     8941 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     9001 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     9061 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     9121 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     9181 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     9241 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     9301 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     9361 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     9421 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     9481 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     9541 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     9601 ccgcgaaat
//
