LOCUS       sequence-22291-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Tee AR, Manning BD, Roux PP, Cantley LC, Blenis J
  TITLE     Tuberous sclerosis complex gene products, Tuberin and Hamartin, 
            control mTOR signaling by acting as a GTPase-activating protein 
            complex toward Rheb.
  JOURNAL   Curr Biol. 2003 Aug 5. 13(15):1259-68.
  PUBMED    12906785
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Aug 5. 13(15):1259-68."
COMMENT     Sequence Label: pGEX-4T2-Rheb
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(657..679)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gatctggttc cgcgtggatc catgccgcag tccaagtccc ggaagatcgc gatcctgggc
       61 taccggtctg tggggaaatc ctcattgacg attcaatttg ttgaaggcca atttgtggac
      121 tcctacgatc caaccataga aaacactttt acaaagttga tcacagtaaa tggacaagaa
      181 tatcatcttc aacttgtaga cacagccggg caagatgaat attctatctt tcctcagaca
      241 tactccatag atattaatgg ctatattctt gtgtattctg ttacatcaat caaaagtttt
      301 gaagtgatta aagttatcca tggcaaattg ttggatatgg tggggaaagt acaaatacct
      361 attatgttgg ttgggaataa gaaagacctg catatggaaa gggtgatcag ttatgaagaa
      421 gggaaagctt tggcagaatc ttggaatgca gcttttttgg aatcttctgc taaagaaaat
      481 cagactgctg tggatgtttt tcgaaggata attttggagg cagaaaaaat ggacggggca
      541 gcttcacaag gcaagtcttc atgctcggtg atgtgagaat tcccgggtcg actcgagcgg
      601 ccgcatcgtg actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
      661 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      721 caagcccgtc agggcgcgtc a
//
