LOCUS       sequence-222918-       16133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Doxycycline-inducible expression of ICP8 synaptase, with red 
            fluorescent protein gene E2-Crimson-P2A-ICP8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Valledor M, Myers RS, Schiller PC
  TITLE     Herpes ICP8 protein stimulates homologous recombination in human 
            cells.
  JOURNAL   PLoS One. 2018 Aug 15;13(8):e0200955. doi: 
            10.1371/journal.pone.0200955. eCollection 2018.
  PUBMED    30110337
REFERENCE   2  (bases 1 to 16133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-08-15"; volume: "13(8):e0200955. doi"; pages: " 
            10.1371/journal.pone.0200955. eCollection 2018"
COMMENT     Sequence Label: Crimson/P2A-ICP8 (pSLIK1)
FEATURES             Location/Qualifiers
     source          1..16133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2679..2697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2716..2734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2790..2808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2827..2845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2901..2919
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2978..3002
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3080..3089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..3760
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     3484..3503
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3770..3826
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(7470..7494)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7544..8755
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8592..8611
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(8814..9431,9432..9437,9438..9473,9474..9476,9477..9512,
                     9513..9515,9516..9551,9552..9560)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9613..10185
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9779..9796)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10006..10025
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        10207..10254
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10273..10647
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    10821..11409
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10874..10894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11292..11303)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11412..11428)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11413..11429)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11938..12118
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12144..12161)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12150..12374
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12420..12848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12507..12526)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12717..12738
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12857..12877)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12862..13191
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13042..13177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        13239..13286
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13305..13679
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13809..13930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13846..13865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13900..13919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13979..13995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13979..13995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13992..14014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14003..14019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14027..14033,14034..14051,14052..14057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14072..14093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14210..14227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14381..14969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14461..14480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15140..15931,15932..16000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15763..15782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16001..16105)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggctgg
     2641 cgccggaacc aattcagtat caagtgccac ctgacgtctc cctatcagtg atagagaagt
     2701 cgacacgtct cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc
     2761 agtgatagag aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag
     2821 aacgtctccc tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga
     2881 gaaggtacgt ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc
     2941 ctatcagtga tagagaaggt accccctata taagcagagc tcgtttagtg aaccgtcaga
     3001 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     3061 gcctggatct aggaggtccg ccaccatgga tagcactgag aacgtcatca agcccttcat
     3121 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcgt
     3181 gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaagtga ccaagggcgg
     3241 ccccctgccc ttcgcctggg acatcctgtc cccccagttc ttctacggct ccaaggcgta
     3301 catcaagcac cccgccgaca tccccgacta cctcaagcag tccttccccg agggcttcaa
     3361 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3421 cctgcaggac ggcaccctca tctaccacgt gaagttcatc ggcgtgaact tcccctccga
     3481 cggccccgta atgcagaaga agactctggg ctgggagccc tccactgagc gcaactaccc
     3541 ccgcgacggc gtgctgaagg gcgagaacca catggcgctg aagctgaagg gcggcggcca
     3601 ctacctgtgt gagttcaagt ccatctacat ggccaagaag cccgtgaagc tgcccggcta
     3661 ccactacgtg gactacaagc tcgacatcac ctcccacaac gaggactaca ccgtggtgga
     3721 gcagtacgag cgcgccgagg cccgccacca cctgttccag ggatccggag ccacgaactt
     3781 ctctctgtta aagcaagcag gagacgtgga agaaaacccc ggtcctcaca tgtctgcgat
     3841 cgccatggag acaaagccca agacggcaac caccatcaag gtcccccccg ggcccctggg
     3901 atacgtgtac gctcgcgcgt gtccgtccga aggcatcgag cttctggcgt tactgtcggc
     3961 gcgcagcggc gatgccgacg tcgccgtggc gcccctggtc gtgggcctga ccgtggagag
     4021 cggctttgag gccaacgtag ccgtggtcgt gggttctcgc acgacggggc tcgggggtac
     4081 cgcggtgtcc ctgaaactga cgccatcgca ctacagctcg tccgtgtacg tctttcacgg
     4141 cggccggcac ctggacccca gcacccaggc cccaaacctg acgcgactct gcgagcgggc
     4201 acgccgccat tttggctttt cggactacac cccccggccc ggcgacctca aacacgagac
     4261 gacgggggag gcgctgtgtg agcgcctcgg cctggacccg gaccgcgccc tcctgtatct
     4321 ggtcgttacc gagggcttca aggaggccgt gtgcatcaac aacacctttc tgcacctggg
     4381 aggctcggac aaggtaacca taggcggggc ggaggtgcac cgcatacccg tgtatccgtt
     4441 gcagctgttc atgccggatt ttagccgggt catcgccgag ccgttcaacg ccaaccaccg
     4501 atcgatcggg gagaatttta cctacccgct tccgtttttt aaccgccccc tcaaccgcct
     4561 cctgttcgag gcggtcgtgg gacccgccgc cgtggcactg cgatgccgaa acgtggacgc
     4621 cgtggcccgc gcggccgccc acctggcgtt tgacgaaaac cacgagggcg ccgccctccc
     4681 cgccgacatt acgttcacgg ccttcgaagc cagccagggt aagaccccgc ggggtgggcg
     4741 cgacggcggc ggcaagggcc cggcgggcgg gttcgaacag cgcctggcct ccgtcatggc
     4801 cggagacgcc gccctggccc tcgagtctat cgtgtcgatg gccgtcttcg acgagccgcc
     4861 caccgacatc tccgcgtggc cgctgtgcga gggccaggac acggccgcgg cccgcgccaa
     4921 cgccgtcggg gcgtacctgg cgcgcgccgc gggactcgtg ggggccatgg tatttagcac
     4981 caactcggcc ctccatctca ccgaggtgga cgacgccggt ccggcggacc caaaggacca
     5041 cagcaaaccc tccttttacc gcttcttcct cgtgcccggg acccacgtgg cggccaaccc
     5101 acaggtggac cgcgagggac acgtggtgcc cgggttcgag ggtcggccca ccgcgcccct
     5161 cgtcggcgga acccaggaat ttgccggcga gcacctggcc atgctgtgtg ggttttcccc
     5221 ggcgctgctg gccaagatgc tgttttacct ggagcgctgc gacggcggcg tgatcgtcgg
     5281 gcgccaggag atggacgtgt ttcgatacgt cgcggactcc aaccagaccg acgtgccctg
     5341 caacctgtgc accttcgaca cgcgccacgc ctgcgtacac acgacgctca tgcgcctccg
     5401 ggcgcgccat cccaagttcg ccagcgccgc ccgcggagcc atcggcgtct tcgggaccat
     5461 gaacagcatg tacagcgact gcgacgtgct gggaaactac gccgccttct cggccctgaa
     5521 gcgcgcggac ggatccgaga ccgcccggac catcatgcag gagacgtacc gcgcggcgac
     5581 cgagcgcgtc atggccgaac tcgagaccct gcagtacgtg gaccaggcgg tccccacggc
     5641 catggggcgg ctggagacca tcatcaccaa ccgcgaggcc ctgcatacgg tggtgaacaa
     5701 cgtcaggcag gtcgtggacc gcgaggtgga gcagctgatg cgcaacctgg tggaggggag
     5761 gaacttcaag tttcgcgacg gtctgggcga ggccaaccac gccatgtccc tgacgctgga
     5821 cccgtacgcg tgcgggccat gccccctgct tcagcttctc gggcggcgat ccaacctcgc
     5881 cgtgtatcag gacctggccc tgagccagtg ccacggggtg ttcgccgggc agtcggtcga
     5941 ggggcgcaac tttcgcaatc aattccaacc ggtgctgcgg cggcgcgtga tggacatgtt
     6001 taacaacggg tttctgtcgg ccaaaacgct gacggtcgcg ctctcggagg gggcggctat
     6061 ctgcgccccc agcctaacgg ccggccagac ggcccccgcc gagagcagct tcgagggcga
     6121 cgttgcccgc gtgaccctgg ggtttcccaa ggagctgcgc gtcaagagcc gcgtgttgtt
     6181 cgcgggcgcg agcgccaacg cgtccgaggc cgccaaggcg cgggtcgcca gcctccagag
     6241 cgcctaccag aagcccgaca agcgcgtgga catcctcctc ggaccgctgg gctttctgct
     6301 gaagcagttc cacgcggcca tcttccccaa cggcaagccc ccggggtcca accagccgaa
     6361 cccgcagtgg ttctggacgg ccctccaacg caaccagctt cccgcccggc tcctgtcgcg
     6421 cgaggacatc gagaccatcg cgttcattaa aaagttttcc ctggactacg gcgcgataaa
     6481 ctttattaac ctggccccca acaacgtgag cgagctggcg atgtactaca tggcaaacca
     6541 gattctgcgg tactgcgatc actcgacata cttcatcaac accctcacgg ccatcatcgc
     6601 ggggtcccgc cgtcccccca gcgtgcaggc ggcggccgcg tggtccgcgc agggcggggc
     6661 gggcctggag gccggggccc gcgcgctgat ggacgccgtg gacgcgcatc cgggcgcgtg
     6721 gacgtccatg ttcgccagct gcaacctgct gcggcccgtc atggcggcgc gccccatggt
     6781 cgtgttgggg ttgagcatca gcaaatacta cggcatggcc ggcaacgacc gtgtgtttca
     6841 ggccgggaac tgggccagcc tgatgggcgg caaaaacgcg tgcccgctcc ttatttttga
     6901 ccgcacccgc aagttcgtcc tggcctgtcc ccgggccggg tttgtgtgcg cggcctcgaa
     6961 cctcggcggc ggagcgcacg aaagctcgct gtgcgagcag ctccggggca ttatctccga
     7021 gggcggggcg gccgtcgcca gtagcgtgtt cgtggcgacc gtgaaaagcc tggggccccg
     7081 cacccagcag ctgcagatcg aggactggct ggcgctcctg gaggacgagt acctaagcga
     7141 ggagatgatg gagctgaccg cgcgtgccct ggagcgcggc aacggcgagt ggtcgacgga
     7201 cgcggccctg gaggtggcgc acgaggccga ggccctagtc agccaactcg gcaacgccgg
     7261 ggaggtgttt aactttgggg attttggctg cgaggacgac aacgcgacgc cgttcggcgg
     7321 cccgggggcc ccgggaccgg catttgccgg ccgcaaacgg gcgttccacg gggatgaccc
     7381 gtttggggag gggccccccg acaaaaaggg agacctgacg ttggatatgc tggtttaaac
     7441 gaattcgcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag tggttgataa
     7501 catctcgagc cataaagatg gttaattaac ccacccaaga tctggcctcc gcgccgggtt
     7561 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     7621 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     7681 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     7741 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     7801 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     7861 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
     7921 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
     7981 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
     8041 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
     8101 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
     8161 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     8221 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     8281 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     8341 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     8401 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     8461 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     8521 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     8581 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     8641 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     8701 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc
     8761 ttggtaccga gctcggatct ccaccccgta ccggtcctgc agtcgaattc accatgtcta
     8821 gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg
     8881 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     8941 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     9001 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     9061 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     9121 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     9181 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     9241 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     9301 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg
     9361 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     9421 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     9481 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     9541 ttgacatgct ccccgggtaa ctaagtaagg atccgcggcc gcactagagg aattccgccc
     9601 ctctcccccc cccccctctc cctccccccc cctaacgtta ctggccgaag ccgcttggaa
     9661 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     9721 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     9781 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     9841 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     9901 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     9961 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
    10021 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
    10081 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
    10141 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagctta ccggtacgcg
    10201 tgatgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
    10261 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
    10321 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
    10381 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
    10441 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
    10501 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
    10561 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
    10621 cacttcgtgg ccgaggagca ggactgacac atctgtacaa gtaaagcggc cgcgactcta
    10681 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    10741 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgtttagt ccctcccaat
    10801 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
    10861 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    10921 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    10981 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    11041 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    11101 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    11161 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
    11221 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    11281 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    11341 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    11401 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
    11461 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
    11521 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
    11581 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
    11641 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
    11701 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
    11761 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
    11821 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
    11881 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
    11941 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    12001 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    12061 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
    12121 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    12181 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    12241 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    12301 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    12361 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
    12421 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    12481 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    12541 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    12601 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    12661 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    12721 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    12781 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    12841 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    12901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
    12961 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
    13021 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    13081 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
    13141 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
    13201 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
    13261 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
    13321 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
    13381 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
    13441 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
    13501 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
    13561 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
    13621 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
    13681 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    13741 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    13801 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    13861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    13921 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    13981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    14041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    14101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    14161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    14221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    14281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    14341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    14401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    14461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    14521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    14581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    14641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    14701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    14761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    14821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    14881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    14941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    15001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    15061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    15121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    15181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    15241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    15301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    15361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    15421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    15481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    15541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    15601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    15661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    15721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    15781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    15841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    15901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    15961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    16021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    16081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
