LOCUS       sequence-222920-       12524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An isogenic synaptase-free control vector to evaluate recombination 
            using endogenous functions for N-terminal Crimson/P2A fusion 
            plasmids..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12524)
  AUTHORS   Valledor M, Myers RS, Schiller PC
  TITLE     Herpes ICP8 protein stimulates homologous recombination in human 
            cells.
  JOURNAL   PLoS One. 2018 Aug 15;13(8):e0200955. doi: 
            10.1371/journal.pone.0200955. eCollection 2018.
  PUBMED    30110337
REFERENCE   2  (bases 1 to 12524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-08-15"; volume: "13(8):e0200955. doi"; pages: " 
            10.1371/journal.pone.0200955. eCollection 2018"
COMMENT     Sequence Label: Crimson/P2A-Control (pSLIK3)
FEATURES             Location/Qualifiers
     source          1..12524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2679..2697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2716..2734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2790..2808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2827..2845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2901..2919
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2978..3002
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3080..3089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..3760
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     3484..3503
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3770..3826
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(3862..3886)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3936..5147
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4984..5003
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5206..5823,5824..5829,5830..5865,5866..5868,5869..5904,
                     5905..5907,5908..5943,5944..5952)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6004..6576
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6170..6187)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6397..6416
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        6598..6645
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6664..7038
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7212..7800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7265..7285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7683..7694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7803..7819)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7804..7820)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8329..8509
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8535..8552)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8541..8765
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8811..9239
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8898..8917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9108..9129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9248..9268)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9253..9582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9433..9568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9495..9514
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9630..9677
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9696..10070
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10200..10321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10237..10256)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10291..10310
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10370..10386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10370..10386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10383..10405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10394..10410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10418..10424,10425..10442,10443..10448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10463..10484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10601..10618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10772..11360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10852..10871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11531..12322,12323..12391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12154..12173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12392..12496)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggctgg
     2641 cgccggaacc aattcagtat caagtgccac ctgacgtctc cctatcagtg atagagaagt
     2701 cgacacgtct cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc
     2761 agtgatagag aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag
     2821 aacgtctccc tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga
     2881 gaaggtacgt ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc
     2941 ctatcagtga tagagaaggt accccctata taagcagagc tcgtttagtg aaccgtcaga
     3001 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     3061 gcctggatct aggaggtccg ccaccatgga tagcactgag aacgtcatca agcccttcat
     3121 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcgt
     3181 gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaagtga ccaagggcgg
     3241 ccccctgccc ttcgcctggg acatcctgtc cccccagttc ttctacggct ccaaggcgta
     3301 catcaagcac cccgccgaca tccccgacta cctcaagcag tccttccccg agggcttcaa
     3361 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3421 cctgcaggac ggcaccctca tctaccacgt gaagttcatc ggcgtgaact tcccctccga
     3481 cggccccgta atgcagaaga agactctggg ctgggagccc tccactgagc gcaactaccc
     3541 ccgcgacggc gtgctgaagg gcgagaacca catggcgctg aagctgaagg gcggcggcca
     3601 ctacctgtgt gagttcaagt ccatctacat ggccaagaag cccgtgaagc tgcccggcta
     3661 ccactacgtg gactacaagc tcgacatcac ctcccacaac gaggactaca ccgtggtgga
     3721 gcagtacgag cgcgccgagg cccgccacca cctgttccag ggatccggag ccacgaactt
     3781 ctctctgtta aagcaagcag gagacgtgga agaaaacccc ggtcctcact gagaattcgc
     3841 ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat aacatctcga
     3901 gccataaaga tggttaatta acccacccaa gatctggcct ccgcgccggg ttttggcgcc
     3961 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
     4021 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
     4081 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
     4141 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
     4201 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
     4261 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
     4321 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
     4381 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
     4441 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
     4501 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
     4561 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     4621 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     4681 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
     4741 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     4801 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     4861 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     4921 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     4981 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     5041 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     5101 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttggtacc
     5161 gagctcggat ctccaccccg taccggtcct gcagtcgaat tcaccatgtc tagactggac
     5221 aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg
     5281 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     5341 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     5401 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     5461 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     5521 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     5581 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     5641 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     5701 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     5761 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     5821 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     5881 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     5941 ctccccgggt aactaagtaa ggatccgcgg ccgcactaga ggaattccgc ccctctcccc
     6001 ccccccctct ccctaccmsc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6061 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6121 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6181 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6241 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6301 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6361 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6421 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6481 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6541 ggggacgtgg ttttcctttg aaaaacacga tgataagctt accggtacgc gtgatgtgtt
     6601 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     6661 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     6721 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     6781 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     6841 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     6901 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     6961 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     7021 gccgaggagc aggactgaca catctgtaca agtaaagcgg ccgcgactct agatcataat
     7081 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7141 gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa ttcgatatca
     7201 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7261 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7321 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7381 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7441 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7501 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7561 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7621 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7681 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7741 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7801 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     7861 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     7921 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7981 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     8041 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     8101 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     8161 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     8221 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     8281 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     8341 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8401 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8461 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     8521 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     8581 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     8641 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     8701 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     8761 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     8821 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8881 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8941 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9001 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9061 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9121 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9181 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     9241 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     9301 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9361 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     9421 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9481 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     9541 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     9601 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     9661 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     9721 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     9781 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     9841 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     9901 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     9961 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
    10021 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
    10081 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    10141 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
    10201 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10261 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10321 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
    10381 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10441 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    10501 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10561 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    11221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    11641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    12001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    12061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    12121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
