LOCUS       sequence-222923-         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of wild-type human Tumor Protein p53 (TP53).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2018-10"; volume: "50"; issue: "10"; pages: 
            "1381-1387"
COMMENT     Sequence Label: pLX313-TP53-WT
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..140)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    148..172
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attaaacagg tccatccttt tccaaatttt gtgatccaga ggttgattgt cgacttaacg
       61 cgttgattgt cgacttaacc ggtactactt cattactacg tagaatcgag accgaggaga
      121 gggttaggga taggcttacc gatatcaacc actttgtaca agaaagttgg gcagtctgag
      181 tcaggccctt ctgtcttgaa catgagtttt ttatggcggg aggtagactg accctttttg
      241 gacttcaggt ggctggagtg agccctgctc ccccctggct ccttcccagc ctgggcatcc
      301 ttgagttcca aggcctcatt cagctctcgg aacatctcga agcgctcacg cccacggatc
      361 tgaagggtga aatattctcc atccagtggt ttcttctttg gctggggaga ggagctggtg
      421 ttgttgggca gtgctcgctt agtgctccct gggggcagct cgtggtgagg ctcccctttc
      481 ttgcggagat tcccttcctc tgtgcgccgg tctctcccag gacaggcaca accacgctcc
      541 tcacagctgt tccgtcccaa tagattacca ctgtagtctt ctttgtgatg atggtgcgga
      601 t
//
