LOCUS       sequence-222928-        1129 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2018-10"; volume: "50"; issue: "10"; pages: 
            "1381-1387"
COMMENT     Sequence Label: pLX313-Firefly luciferase
FEATURES             Location/Qualifiers
     source          1..1129
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(135..153)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ctttcctttt aatgtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
       61 ttggcaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
      121 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
      181 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
      241 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
      301 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
      361 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
      421 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
      481 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
      541 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
      601 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
      661 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
      721 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
      781 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
      841 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
      901 agattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag
      961 cactctgatt gacaagtacg atttatctaa tttacacgaa ttgcttctgg tgcgctcccc
     1021 tctctaagaa gtcggggagc gttgcccaga gtcatctgcc agtatngnag atatgggcnn
     1081 tggagactac tcagctatcg attaccctcc gaggcgggat gaatacccg
//
