LOCUS       sequence-222931-         440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2018-10"; volume: "50"; issue: "10"; pages: 
            "1381-1387"
COMMENT     Sequence Label: pLX311-Cas9
FEATURES             Location/Qualifiers
     source          1..440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..141)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    149..173
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(176..223)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttaaaatccg gttcatcctt ttccaaattt tgtaatcctg aggttgattg tcgacttacc
       61 gcgttgattg tcgacttaac cggtactagt tcattactac gtagaatcga gaccgaggag
      121 agggttaggg ataggcttac cgatatcaac cactttgtac aagaaagttg ggggtctttt
      181 tcttttttgc ctggccggcc tttttcgtgg ccgccggcct tttgtcgcct cccagctgag
      241 acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg gtggatcagg gtggcgtcca
      301 gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg
      361 cggcaggggc tcccagattg gtcagggtaa acaggtggat gatattctcg gtctgctctc
      421 tgatgggctt atcccggtgc
//
