LOCUS       sequence-222937-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of sgRNA targeting human TP53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2018-10"; volume: "50"; issue: "10"; pages: 
            "1381-1387"
COMMENT     Sequence Label: pXPR003-sgTP53-5
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        66..141
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    197..314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcatgt gtaacagttc
       61 ctgcagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      121 aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg
      181 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      241 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      301 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
      361 agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
      421 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
      481 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct
      541 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      601 ccgagggtgg gggagaaccg tatataagtg cactattcgc cgtgaacgtt ctttttcgca
      661 acgggttttg ccgcccgaa
//
