LOCUS       sequence-222948-       16017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Doxycycline-inducible expression of ICP8 synaptase gene, with 
            ICP8-P2A-red fluorescent protein gene E2-Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16017)
  AUTHORS   Valledor M, Myers RS, Schiller PC
  TITLE     Herpes ICP8 protein stimulates homologous recombination in human 
            cells.
  JOURNAL   PLoS One. 2018 Aug 15;13(8):e0200955. doi: 
            10.1371/journal.pone.0200955. eCollection 2018.
  PUBMED    30110337
REFERENCE   2  (bases 1 to 16017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-08-15"; volume: "13(8):e0200955. doi"; pages: " 
            10.1371/journal.pone.0200955. eCollection 2018"
COMMENT     Sequence Label: ICP8-P2A/Crimson (pSLIK4)
FEATURES             Location/Qualifiers
     source          1..16017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2679..2697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2716..2734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2790..2808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2827..2845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2904..2928
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6602..6658
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6659..7336
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     7057..7076
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(7370..7394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7444..8655
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8492..8511
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(8714..9331,9332..9337,9338..9373,9374..9376,9377..9412,
                     9413..9415,9416..9451,9452..9460)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9498..10069
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9663..9680)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9890..9909
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        10091..10138
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10157..10531
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    10705..11293
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10758..10778)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11176..11187)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11296..11312)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11297..11313)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11822..12002
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12028..12045)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12034..12258
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12304..12732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12391..12410)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12601..12622
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12741..12761)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12746..13075
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12926..13061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        13123..13170
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13189..13563
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13693..13814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13730..13749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13784..13803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13863..13879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13863..13879)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13876..13898)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13887..13903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13911..13917,13918..13935,13936..13941))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13956..13977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14094..14111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14265..14853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14345..14364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15024..15815,15816..15884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15647..15666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15885..15989)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggctgg
     2641 cgccggaacc aattcagtat caagtgccac ctgacgtctc cctatcagtg atagagaagt
     2701 cgacacgtct cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc
     2761 agtgatagag aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag
     2821 aacgtctccc tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga
     2881 gaaggtaccc cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2941 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ggatcgccat
     3001 ggagacaaag cccaagacgg caaccaccat caaggtcccc cccgggcccc tgggatacgt
     3061 gtacgctcgc gcgtgtccgt ccgaaggcat cgagcttctg gcgttactgt cggcgcgcag
     3121 cggcgatgcc gacgtcgccg tggcgcccct ggtcgtgggc ctgaccgtgg agagcggctt
     3181 tgaggccaac gtagccgtgg tcgtgggttc tcgcacgacg gggctcgggg gtaccgcggt
     3241 gtccctgaaa ctgacgccat cgcactacag ctcgtccgtg tacgtctttc acggcggccg
     3301 gcacctggac cccagcaccc aggccccaaa cctgacgcga ctctgcgagc gggcacgccg
     3361 ccattttggc ttttcggact acaccccccg gcccggcgac ctcaaacacg agacgacggg
     3421 ggaggcgctg tgtgagcgcc tcggcctgga cccggaccgc gccctcctgt atctggtcgt
     3481 taccgagggc ttcaaggagg ccgtgtgcat caacaacacc tttctgcacc tgggaggctc
     3541 ggacaaggta accataggcg gggcggaggt gcaccgcata cccgtgtatc cgttgcagct
     3601 gttcatgccg gattttagcc gggtcatcgc cgagccgttc aacgccaacc accgatcgat
     3661 cggggagaat tttacctacc cgcttccgtt ttttaaccgc cccctcaacc gcctcctgtt
     3721 cgaggcggtc gtgggacccg ccgccgtggc actgcgatgc cgaaacgtgg acgccgtggc
     3781 ccgcgcggcc gcccacctgg cgtttgacga aaaccacgag ggcgccgccc tccccgccga
     3841 cattacgttc acggccttcg aagccagcca gggtaagacc ccgcggggtg ggcgcgacgg
     3901 cggcggcaag ggcccggcgg gcgggttcga acagcgcctg gcctccgtca tggccggaga
     3961 cgccgccctg gccctcgagt ctatcgtgtc gatggccgtc ttcgacgagc cgcccaccga
     4021 catctccgcg tggccgctgt gcgagggcca ggacacggcc gcggcccgcg ccaacgccgt
     4081 cggggcgtac ctggcgcgcg ccgcgggact cgtgggggcc atggtattta gcaccaactc
     4141 ggccctccat ctcaccgagg tggacgacgc cggtccggcg gacccaaagg accacagcaa
     4201 accctccttt taccgcttct tcctcgtgcc cgggacccac gtggcggcca acccacaggt
     4261 ggaccgcgag ggacacgtgg tgcccgggtt cgagggtcgg cccaccgcgc ccctcgtcgg
     4321 cggaacccag gaatttgccg gcgagcacct ggccatgctg tgtgggtttt ccccggcgct
     4381 gctggccaag atgctgtttt acctggagcg ctgcgacggc ggcgtgatcg tcgggcgcca
     4441 ggagatggac gtgtttcgat acgtcgcgga ctccaaccag accgacgtgc cctgcaacct
     4501 gtgcaccttc gacacgcgcc acgcctgcgt acacacgacg ctcatgcgcc tccgggcgcg
     4561 ccatcccaag ttcgccagcg ccgcccgcgg agccatcggc gtcttcggga ccatgaacag
     4621 catgtacagc gactgcgacg tgctgggaaa ctacgccgcc ttctcggccc tgaagcgcgc
     4681 ggacggatcc gagaccgccc ggaccatcat gcaggagacg taccgcgcgg cgaccgagcg
     4741 cgtcatggcc gaactcgaga ccctgcagta cgtggaccag gcggtcccca cggccatggg
     4801 gcggctggag accatcatca ccaaccgcga ggccctgcat acggtggtga acaacgtcag
     4861 gcaggtcgtg gaccgcgagg tggagcagct gatgcgcaac ctggtggagg ggaggaactt
     4921 caagtttcgc gacggtctgg gcgaggccaa ccacgccatg tccctgacgc tggacccgta
     4981 cgcgtgcggg ccatgccccc tgcttcagct tctcgggcgg cgatccaacc tcgccgtgta
     5041 tcaggacctg gccctgagcc agtgccacgg ggtgttcgcc gggcagtcgg tcgaggggcg
     5101 caactttcgc aatcaattcc aaccggtgct gcggcggcgc gtgatggaca tgtttaacaa
     5161 cgggtttctg tcggccaaaa cgctgacggt cgcgctctcg gagggggcgg ctatctgcgc
     5221 ccccagccta acggccggcc agacggcccc cgccgagagc agcttcgagg gcgacgttgc
     5281 ccgcgtgacc ctggggtttc ccaaggagct gcgcgtcaag agccgcgtgt tgttcgcggg
     5341 cgcgagcgcc aacgcgtccg aggccgccaa ggcgcgggtc gccagcctcc agagcgccta
     5401 ccagaagccc gacaagcgcg tggacatcct cctcggaccg ctgggctttc tgctgaagca
     5461 gttccacgcg gccatcttcc ccaacggcaa gcccccgggg tccaaccagc cgaacccgca
     5521 gtggttctgg acggccctcc aacgcaacca gcttcccgcc cggctcctgt cgcgcgagga
     5581 catcgagacc atcgcgttca ttaaaaagtt ttccctggac tacggcgcga taaactttat
     5641 taacctggcc cccaacaacg tgagcgagct ggcgatgtac tacatggcaa accagattct
     5701 gcggtactgc gatcactcga catacttcat caacaccctc acggccatca tcgcggggtc
     5761 ccgccgtccc cccagcgtgc aggcggcggc cgcgtggtcc gcgcagggcg gggcgggcct
     5821 ggaggccggg gcccgcgcgc tgatggacgc cgtggacgcg catccgggcg cgtggacgtc
     5881 catgttcgcc agctgcaacc tgctgcggcc cgtcatggcg gcgcgcccca tggtcgtgtt
     5941 ggggttgagc atcagcaaat actacggcat ggccggcaac gaccgtgtgt ttcaggccgg
     6001 gaactgggcc agcctgatgg gcggcaaaaa cgcgtgcccg ctccttattt ttgaccgcac
     6061 ccgcaagttc gtcctggcct gtccccgggc cgggtttgtg tgcgcggcct cgaacctcgg
     6121 cggcggagcg cacgaaagct cgctgtgcga gcagctccgg ggcattatct ccgagggcgg
     6181 ggcggccgtc gccagtagcg tgttcgtggc gaccgtgaaa agcctggggc cccgcaccca
     6241 gcagctgcag atcgaggact ggctggcgct cctggaggac gagtacctaa gcgaggagat
     6301 gatggagctg accgcgcgtg ccctggagcg cggcaacggc gagtggtcga cggacgcggc
     6361 cctggaggtg gcgcacgagg ccgaggccct agtcagccaa ctcggcaacg ccggggaggt
     6421 gtttaacttt ggggattttg gctgcgagga cgacaacgcg acgccgttcg gcggcccggg
     6481 ggccccggga ccggcatttg ccggccgcaa acgggcgttc cacggggatg acccgtttgg
     6541 ggaggggccc cccgacaaaa agggagacct gacgttggat atgctggttt ccggatccgg
     6601 agccacgaac ttctctctgt taaagcaagc aggagacgtg gaagaaaacc ccggtcctat
     6661 ggatagcact gagaacgtca tcaagccctt catgcgcttc aaggtgcaca tggagggctc
     6721 cgtgaacggc cacgagttcg agatcgaggg cgtgggcgag ggcaagccct acgagggcac
     6781 ccagaccgcc aagctgcaag tgaccaaggg cggccccctg cccttcgcct gggacatcct
     6841 gtccccccag ttcttctacg gctccaaggc gtacatcaag caccccgccg acatccccga
     6901 ctacctcaag cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     6961 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcaccc tcatctacca
     7021 cgtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga agaagactct
     7081 gggctgggag ccctccactg agcgcaacta cccccgcgac ggcgtgctga agggcgagaa
     7141 ccacatggcg ctgaagctga agggcggcgg ccactacctg tgtgagttca agtccatcta
     7201 catggccaag aagcccgtga agctgcccgg ctaccactac gtggactaca agctcgacat
     7261 cacctcccac aacgaggact acaccgtggt ggagcagtac gagcgcgccg aggcccgcca
     7321 ccacctgttc cagtagaaac gaattcgcgg ccgcactcga gatatctaga cccagctttc
     7381 ttgtacaaag tggttgataa catctcgagc cataaagatg gttaattaac ccacccaaga
     7441 tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg
     7501 ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga
     7561 cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt
     7621 taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg
     7681 aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc
     7741 gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg
     7801 ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg
     7861 gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac
     7921 cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga
     7981 gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg
     8041 tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc
     8101 cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac
     8161 cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc
     8221 agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt
     8281 gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt
     8341 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
     8401 aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg
     8461 tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg
     8521 gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg
     8581 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
     8641 gcttttttgt tagacgaagc ttggtaccga gctcggatct ccaccccgta ccggtcctgc
     8701 agtcgaattc accatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt
     8761 actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     8821 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     8881 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     8941 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     9001 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     9061 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     9121 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     9181 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     9241 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     9301 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     9361 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     9421 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atccgcggcc
     9481 gcactagagg aattccgccc ctctcccccc cccccctaac gttactggcc gaagccgctt
     9541 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     9601 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     9661 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     9721 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     9781 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     9841 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     9901 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     9961 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
    10021 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttaccggta
    10081 cgcgtgatgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
    10141 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
    10201 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
    10261 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
    10321 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
    10381 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
    10441 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
    10501 cgtgcacttc gtggccgagg agcaggactg acacatctgt acaagtaaag cggccgcgac
    10561 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    10621 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc
    10681 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
    10741 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    10801 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    10861 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    10921 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    10981 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    11041 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    11101 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    11161 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    11221 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    11281 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
    11341 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
    11401 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
    11461 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
    11521 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
    11581 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
    11641 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
    11701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
    11761 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
    11821 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    11881 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11941 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    12001 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
    12061 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
    12121 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
    12181 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
    12241 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
    12301 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    12361 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    12421 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    12481 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    12541 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    12601 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    12661 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    12721 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    12781 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    12841 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    12901 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    12961 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
    13021 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    13081 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
    13141 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
    13201 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
    13261 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
    13321 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
    13381 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
    13441 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
    13501 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    13561 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    13621 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    13681 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    13741 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    13801 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    13861 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    13921 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    13981 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    14041 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    14101 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    14161 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    14221 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    14281 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    14341 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    14401 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    14461 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    14521 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    14581 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    14641 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    14701 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    14761 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    14821 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    14881 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    14941 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    15001 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    15061 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    15121 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    15181 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    15241 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    15301 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    15361 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    15421 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    15481 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    15541 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    15601 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    15661 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    15721 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    15781 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    15841 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    15901 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    15961 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
