LOCUS       sequence-22295-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Nobes CD, Hall A
  TITLE     Rho GTPases control polarity, protrusion, and adhesion during cell 
            movement.
  JOURNAL   J Cell Biol. 1999 Mar 22. 144(6):1235-44.
  PUBMED    10087266
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1999 Mar 22. 144(6):1235-44."
COMMENT     Sequence Label: pRK5myc RhoA L63
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..87
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(97..279,280..282,283..675)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="AAIRKKLVIVGDGACGKTCLLIVNSKDQFPEVYVPTVFENYVADI
                     EVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKH
                     FCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTK
                     DGVREVFEMATRAALQARRGKKKSGCLVL"
     regulatory      702..711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(752..771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttgaattggc caccatggag
       61 cagaagctga tctccgagga ggacctggga tccccggctg ccatccggaa gaaactggtg
      121 attgttggtg atggagcctg tggaaagaca tgcttgctca tagttaacag caaggaccag
      181 ttcccagagg tgtatgtgcc cacagtgttt gagaactatg tggcagatat cgaggtggat
      241 ggaaagcagg tagagttggc tttgtgggac acagctgggc tggaagatta tgatcgcctg
      301 aggcccctct cctacccaga taccgatgtt atactgatgt gtttttccat cgacagccct
      361 gatagtttag aaaacatccc agaaaagtgg accccagaag tcaagcattt ctgtcccaac
      421 gtgcccatca tcctggttgg gaataagaag gatcttcgga atgatgagca cacaaggcgg
      481 gagctagcca agatgaagca ggagccggtg aaacctgaag aaggcagaga tatggcaaac
      541 aggattggcg cttttgggta catggagtgt tcagcaaaga ccaaagatgg agtgagagag
      601 gtttttgaaa tggctacgag agctgctctg caagctagac gtgggaagaa aaaatctggg
      661 tgccttgtct tgtgaaagct gggaattcct gcagaagctt ggccgccatg gcccaacttg
      721 tttattgcag ctttatatgg tttacaataa agcaatagca tcacaaattt cacaaataaa
      781 g
//
