LOCUS       sequence-222955-       13416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Doxycycline-inducible expression of Humanized Beta synaptase 
            gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized 
            Beta synaptase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Valledor M, Myers RS, Schiller PC
  TITLE     Herpes ICP8 protein stimulates homologous recombination in human 
            cells.
  JOURNAL   PLoS One. 2018 Aug 15;13(8):e0200955. doi: 
            10.1371/journal.pone.0200955. eCollection 2018.
  PUBMED    30110337
REFERENCE   2  (bases 1 to 13416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-08-15"; volume: "13(8):e0200955. doi"; pages: " 
            10.1371/journal.pone.0200955. eCollection 2018"
COMMENT     Sequence Label: Crimson/P2A-NLS/HA-HumBeta (pSLIK2)
FEATURES             Location/Qualifiers
     source          1..13416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2679..2697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2716..2734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2790..2808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2827..2845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2901..2919
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2978..3002
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3080..3089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..3760
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     3484..3503
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3770..3826
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3839..3859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3872..3898
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4767..4791)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4841..6052
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5889..5908
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6111..6728,6729..6734,6735..6770,6771..6773,6774..6809,
                     6810..6812,6813..6848,6849..6857)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6895..7468
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7062..7079)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7289..7308
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        7490..7537
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7556..7930
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8104..8692
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8157..8177)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8575..8586)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8695..8711)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8696..8712)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9221..9401
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9427..9444)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9433..9657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9703..10131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9790..9809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10000..10021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10140..10160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10145..10474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10325..10460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10387..10406
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10522..10569
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10588..10962
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11092..11213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11129..11148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11183..11202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11262..11278)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11262..11278)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11275..11297)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11286..11302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11310..11316,11317..11334,11335..11340))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11355..11376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11493..11510)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11664..12252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11744..11763)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12423..13214,13215..13283))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13046..13065
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13284..13388)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggctgg
     2641 cgccggaacc aattcagtat caagtgccac ctgacgtctc cctatcagtg atagagaagt
     2701 cgacacgtct cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc
     2761 agtgatagag aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag
     2821 aacgtctccc tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga
     2881 gaaggtacgt ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc
     2941 ctatcagtga tagagaaggt accccctata taagcagagc tcgtttagtg aaccgtcaga
     3001 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     3061 gcctggatct aggaggtccg ccaccatgga tagcactgag aacgtcatca agcccttcat
     3121 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcgt
     3181 gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaagtga ccaagggcgg
     3241 ccccctgccc ttcgcctggg acatcctgtc cccccagttc ttctacggct ccaaggcgta
     3301 catcaagcac cccgccgaca tccccgacta cctcaagcag tccttccccg agggcttcaa
     3361 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3421 cctgcaggac ggcaccctca tctaccacgt gaagttcatc ggcgtgaact tcccctccga
     3481 cggccccgta atgcagaaga agactctggg ctgggagccc tccactgagc gcaactaccc
     3541 ccgcgacggc gtgctgaagg gcgagaacca catggcgctg aagctgaagg gcggcggcca
     3601 ctacctgtgt gagttcaagt ccatctacat ggccaagaag cccgtgaagc tgcccggcta
     3661 ccactacgtg gactacaagc tcgacatcac ctcccacaac gaggactaca ccgtggtgga
     3721 gcagtacgag cgcgccgagg cccgccacca cctgttccag ggatccggag ccacgaactt
     3781 ctctctgtta aagcaagcag gagacgtgga agaaaacccc ggtcctcaca tggtgcctcc
     3841 caagaagaag aggaaggtcg aggaccccaa gtacccctat gacgtgcccg attacgctgg
     3901 cggaggcgga ggctccggcg gaggaggagg atccggagga ggcagcatgt ccacagctct
     3961 cgccaccctg gccggaaaac tggccgagag agtgggcatg gactccgtgg atccccagga
     4021 gctgatcacc acactgaggc aaaccgcctt caagggagat gcttccgacg cccaattcat
     4081 cgccctcctc atcgtggcca accagtacgg cctgaaccct tggaccaagg agatttacgc
     4141 cttccccgac aagcagaatg gcattgtccc tgtggtcggc gtggacggct ggagcagaat
     4201 cattaacgag aaccagcagt ttgacggcat ggacttcgag caggacaacg agagctgcac
     4261 atgcaggatc tacaggaagg acaggaacca tcctatctgc gtcaccgagt ggatggatga
     4321 gtgcagaaga gaacccttca agaccagaga gggcagagag atcacaggac cctggcagtc
     4381 ccatcctaag agaatgctca gacacaaggc catgatccag tgtgccaggc tcgcttttgg
     4441 cttcgccggc atctatgata aggacgaagc cgagaggatc gtggaaaata ccgcctacac
     4501 cgctgaaagg cagcccgaaa gggacattac acccgtgaac gacgagacca tgcaggagat
     4561 caataccctg ctcattgccc tggacaaaac ctgggacgat gacctcctcc ccctctgcag
     4621 ccaaatcttc aggagggaca tcagggccag ctccgaactc acacaagccg aagctgtgaa
     4681 ggccctcgga ttcctcaaac agaaggccgc tgagcagaag gtcgccgccg tttaaacgaa
     4741 ttcgcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg ttgataacat
     4801 ctcgagccat aaagatggtt aattaaccca cccaagatct ggcctccgcg ccgggttttg
     4861 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
     4921 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
     4981 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
     5041 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
     5101 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
     5161 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
     5221 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
     5281 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
     5341 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
     5401 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
     5461 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
     5521 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     5581 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     5641 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     5701 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     5761 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     5821 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     5881 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     5941 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     6001 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
     6061 gtaccgagct cggatctcca ccccgtaccg gtcctgcagt cgaattcacc atgtctagac
     6121 tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc ggtatcgaag
     6181 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
     6241 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
     6301 atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
     6361 agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
     6421 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
     6481 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
     6541 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
     6601 attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac
     6661 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
     6721 aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg
     6781 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
     6841 acatgctccc cgggtaacta agtaaggatc cgcggccgca ctagaggaat tccgcccctc
     6901 tccccccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     6961 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     7021 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     7081 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     7141 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     7201 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     7261 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     7321 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     7381 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     7441 ggttttcctt tgaaaaacac gatgataagc ttaccggtac gcgtgatgtg ttgacaatta
     7501 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     7561 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     7621 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     7681 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     7741 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     7801 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     7861 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     7921 gcaggactga cacatctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     7981 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8041 aacataaaat gaatgcaatt gttgttgttt agtccctccc aattcgatat caagcttatc
     8101 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     8161 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     8221 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     8281 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     8341 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     8401 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     8461 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     8521 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8581 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8641 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     8701 cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     8761 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     8821 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8881 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     8941 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     9001 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     9061 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     9121 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     9181 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     9241 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9301 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9361 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     9421 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     9481 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     9541 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     9601 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     9661 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     9721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9841 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9901 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9961 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10021 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10081 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    10141 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10201 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10381 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    10441 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    10501 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
    10561 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
    10621 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
    10681 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
    10741 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
    10801 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
    10861 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
    10921 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
    10981 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    11041 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    11101 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    11161 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11221 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    11281 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    11341 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    11401 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    11461 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11521 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11581 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11641 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11761 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11821 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11881 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11941 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    12061 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    12121 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    12181 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    12241 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    12301 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    12361 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    12421 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    12481 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    12541 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    12601 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    12661 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    12721 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    12781 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    12841 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    12901 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    12961 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    13021 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    13081 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    13141 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    13201 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    13261 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    13321 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    13381 gttccgcgca catttccccg aaaagtgcca cctgac
//
