LOCUS       sequence-222960-        6675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_FLAG-Sfxn1-3 (CG11739)
FEATURES             Location/Qualifiers
     source          1..6675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             58..651
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    714..1071
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1130..1546
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1476..1498
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1514..1530
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1556..1930
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1868..1884
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1959..1968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1968..2033
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(3063..3637,3638..3640,3641..3649)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3230..3247)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3457..3476
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3650..4045
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4216..4685
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4815..4832)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4986..5574)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5066..5085)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5738..6529,6530..6598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6361..6380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6599..6675,1..28))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcataat
       61 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
      121 gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacag atggaacagc
      181 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
      241 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
      301 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
      361 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
      421 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
      481 aaagagccca caacccctca ctcggcgcgc cagtcctccg attgactgag tcgcccgggt
      541 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
      601 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc
      661 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
      721 ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt tatgcgcctg
      781 cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag
      841 ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg ccgtttttgt
      901 ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac ccccctaata
      961 ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga
     1021 atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt
     1081 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
     1141 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     1201 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     1261 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     1321 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     1381 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     1441 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     1501 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata
     1561 tgagatctta tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag
     1621 agttactaac agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga
     1681 agtctggaga cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca
     1741 cccttaccga gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc
     1801 tcgctggaaa ggaccttaca cagtcctgct gaccaccccc accgccctca aagtagacgg
     1861 catcgcagct tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc
     1921 ctctagactg ccggatctag ctagttaatt aaggatccgc caccatggac tacaaggacc
     1981 acgacggtga ctacaaggac cacgacatcg actacaagga cgacgacgac aaggggcatg
     2041 gcaccggcag caccggcagc gaattcatgt ctccgcttcc tagagtaaat atagacgaac
     2101 cgaagtatga tcaaaacagc tatttgggca gggcaaaaca cttctttctg ctcactaacc
     2161 ccctgaatgt cctggcttct gccagtaaac tggaggaagc tcgccaaatt gttataaaat
     2221 acagagctgg aaaagatgta ccagagtgta aaactatcga tgatgtttgg agagcaaagt
     2281 atctttatga tagtgccttt catccagaaa ccggagagaa acagatcgtt atcggtagaa
     2341 tggcggccca gatgccaatg aatacgatca tcacaggcgg tatgatggct ttttataagt
     2401 ccacaccggc tgttgtcttc tggcaatggt ttaaccagac ttttaatgcg attgtgaact
     2461 acactaatcg ctctgggaca tcaccgatct cacagcaaca gcttgtaacc tcatattgct
     2521 tggctacatc tggcgccctg gttaccgccc tcagtctcaa ccatgcagta aagaatatga
     2581 accctttgtt ggggcgcctc gttccattgg tagccgtagg tgcggccaac tgcattaaca
     2641 ttccgtgtat gcggatgcaa gaattgagaa acggtgttac gctcttcgat gaacattcta
     2701 atgagatggg aatctccaag aaggctgcag tcgtaggtat ttctacagta atactgtcac
     2761 gaattgccat ggcaatccct ggtatgacgt tgactcccgt attgatgaat gtgttggaga
     2821 aaaggggatt tcttgctaaa taccctagaa gcaatgcacc catacagaca ctcttttgcg
     2881 gtttcgtatt gatctttgca acgccgctcg gatgtgcttt tttcaagcag agggcagaca
     2941 tcaaggttga ctcacttgaa agcgaggtcc gcgattccat tcggaaaaag cgaccagagt
     3001 tggaaaccgt atggttcaat aaaggcctct aagcggccgc tacgtaaatt ccgccccccc
     3061 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3121 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3181 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3241 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3301 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3361 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3421 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3481 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3541 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3601 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggttccttt
     3661 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     3721 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     3781 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     3841 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     3901 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     3961 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     4021 ctctggttat gtgtgggagg gctaagtcga cgataaaata aaagatttta tttagtctcc
     4081 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     4141 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     4201 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     4261 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     4321 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     4381 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     4441 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     4501 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     4561 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     4621 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     4681 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     4741 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4801 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4861 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4921 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4981 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5041 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5101 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5161 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5221 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5281 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5341 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5401 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5461 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5521 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5581 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5641 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat
     5701 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5761 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5821 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5881 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5941 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6001 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6061 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6121 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6181 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6241 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6301 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6361 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6421 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6481 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6541 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6601 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6661 tatttgaatg tattt
//
