LOCUS       sequence-22299-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Nobes CD, Hall A
  TITLE     Rho GTPases control polarity, protrusion, and adhesion during cell 
            movement.
  JOURNAL   J Cell Biol. 1999 Mar 22. 144(6):1235-44.
  PUBMED    10087266
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1999 Mar 22. 144(6):1235-44."
COMMENT     Sequence Label: pRK5myc Rac1 N17
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..87
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(97..141,142..144,145..672)
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="QAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     CDS             97..672
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="QAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
ORIGIN
        1 gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttgaattggc caccatggag
       61 cagaagctga tctccgagga ggacctggga tccccgcagg ccatcaagtg tgtggtggtg
      121 ggagacggag ctgtaggtaa aaattgccta ctgatcagtt acacaaccaa tgcatttcct
      181 ggagaatata tccctactgt ctttgacaat tattctgcca atgttatggt agatggaaaa
      241 ccggtgaatc tgggcttatg ggatacagct ggacaagaag attatgacag attacgcccc
      301 ctatcctatc cgcaaacaga tgtgttctta atttgctttt cccttgtgag tcctgcatca
      361 tttgaaaatg tccgtgcaaa gtggtatcct gaggtgcggc accactgtcc caacactccc
      421 atcatcctag tgggaactaa acttgatctt agggatgata aagacacgat cgagaaactg
      481 aaggagaaga agctgactcc catcacctat ccgcagggtc tagccatggc taaggagatt
      541 ggtgccgtaa aatacctgga gtgctcggcg ctcacacagc gaggcctcaa gacagtgttt
      601 gacgaagcga tccgagcagt cctctgcccg cctcccgtga agaagaggaa gagaaaatgc
      661 ctgctgttgt aagaattcct gcag
//
