LOCUS       sequence-222990-        5469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Pu J, Zinkus-Boltz J, Dickinson BC
  TITLE     Evolution of a split RNA polymerase as a versatile biosensor 
            platform.
  JOURNAL   Nat Chem Biol. 2017 Apr;13(4):432-438. doi: 10.1038/nchembio.2299. 
            Epub 2017 Feb 13.
  PUBMED    28192413
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2299"; journalName: "Nat Chem Biol"; date: 
            "2017-04"; volume: "13"; issue: "4"; pages: "432-438"
COMMENT     Sequence Label: p5-39
FEATURES             Location/Qualifiers
     source          1..5469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(39..777)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     terminator      867..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(927..1805)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1832..2116
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2039..2058
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             join(2166..2168,2169..2489)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     terminator      4649..4680
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(4696..5355)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt
       61 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc
      121 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt
      181 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac
      241 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg
      301 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc
      361 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
      421 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
      481 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg
      541 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt
      601 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt
      661 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg
      721 tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      781 tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
      841 agcccaggag gaagaggaca tccggtcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tagacttagg gaccctttat gacaacttga cggctacatc attcactttt
      961 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
     1021 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
     1081 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     1141 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
     1201 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
     1261 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
     1321 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
     1381 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
     1441 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
     1501 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
     1561 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
     1621 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
     1681 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
     1741 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
     1801 gccatacttt tcatactccc accattcaga gaagaaacca attgtccata ttgcatcaga
     1861 cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt
     1921 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
     1981 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
     2041 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
     2101 tctactgttt ctccataccc gtttttttgg acgcgtacaa ctcaagtctg acataaatga
     2161 ccgctatggg agttcaggta gagacaatta gtcctgggga tggccgcaca tttccgaaac
     2221 gcggccaaac ctgtgtggta cactatacag gaatgttgga agatggcaaa aagtttgatt
     2281 ccagtcgtga tcgcaacaag ccttttaaat ttatgttggg aaaacaggag gtaattcgcg
     2341 gttgggagga aggtgtcgca caaatgtccg tgggtcaacg cgccaagttg acgatctcac
     2401 ctgattacgc ttacggagcc accggccatc ctggcattat tcccccacat gccactttgg
     2461 tctttgatgt tgaactttta aagttagaaa cgtcgggtgg aagcggtaaa gcatttatgc
     2521 aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt
     2581 ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc attgagtcaa
     2641 ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac tctgagacta
     2701 tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca
     2761 tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc attactggtg
     2821 gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag
     2881 cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt aacattgcgc
     2941 aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta atcaccaagt
     3001 ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc ccgatgaaac
     3061 cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg
     3121 tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc atgcttgagc
     3181 aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg gactggcgcg
     3241 gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc
     3301 ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg aaaatccacg
     3361 gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag ttcattgagg
     3421 aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact tggtgggctg
     3481 agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg gtacagcacc
     3541 acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc tctggcatcc
     3601 agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac ttgcttccta
     3661 gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag attctacaag
     3721 cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag aacactggtg
     3781 aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg ctggcttacg
     3841 gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg tccaaagagt
     3901 tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat tccggcaagg
     3961 gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg atttgggaat
     4021 ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag tctgctgcta
     4081 agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc aagcgttgcg
     4141 ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag aagcctattc
     4201 agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc attaacacca
     4261 acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct aactttgtac
     4321 acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa
     4381 tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac gctgcgaacc
     4441 tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat gtactggctg
     4501 atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa atgccagcac
     4561 ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc gcgttcgcgt
     4621 aaacttaatt aacggcactc ctcagccaag tcaaaagcct ccgaccggag gcttttgact
     4681 acatgcccat ggcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4741 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     4801 catcagcacc ttgtcgcctt gcgtataata tttgcccata gtgaaaacgg gggcgaagaa
     4861 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4921 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4981 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     5041 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     5101 ccatatcacc agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag
     5161 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     5221 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag taactgactg
     5281 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     5341 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     5401 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccaagccaa
     5461 ataggccgt
//
