LOCUS       sequence-223010-        9354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PspCas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pC0068	PspCas13 (B12) His6-TwinStrep-SUMO-BsaI
FEATURES             Location/Qualifiers
     source          1..9354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3321..3339)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3335..3382
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(3557..3562,3563..3579,3580..3585))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(3612..3630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3698..3802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3803..3871,3872..4663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4021..4040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4834..5422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5323..5342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5576..5593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5608..5747
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5673..5692)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5833..5855
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5849..6040)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7352..8434)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(8435..8512)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8718..8737
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     8821..8840
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8821..8839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8840..8864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8879..8901
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8920..8937
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8947..8964
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(8977..9000,9001..9036,9037..9060)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             9061..9354
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 tctatgaaca tccccgctct ggtggaaaac cagaagaagt actttggcac ctacagcgtg
       61 atggccatgc tgaacgctca gaccgtgctg gaccacatcc agaaggtggc cgatattgag
      121 ggcgagcaga acgagaacaa cgagaatctg tggtttcacc ccgtgatgag ccacctgtac
      181 aacgccaaga acggctacga caagcagccc gagaaaacca tgttcatcat cgagcggctg
      241 cagagctact tcccattcct gaagatcatg gccgagaacc agagagagta cagcaacggc
      301 aagtacaagc agaaccgcgt ggaagtgaac agcaacgaca tcttcgaggt gctgaagcgc
      361 gccttcggcg tgctgaagat gtacagggac ctgaccaacc actacaagac ctacgaggaa
      421 aagctgaacg acggctgcga gttcctgacc agcacagagc aacctctgag cggcatgatc
      481 aacaactact acacagtggc cctgcggaac atgaacgaga gatacggcta caagacagag
      541 gacctggcct tcatccagga caagcggttc aagttcgtga aggacgccta cggcaagaaa
      601 aagtcccaag tgaataccgg attcttcctg agcctgcagg actacaacgg cgacacacag
      661 aagaagctgc acctgagcgg agtgggaatc gccctgctga tctgcctgtt cctggacaag
      721 cagtacatca acatctttct gagcaggctg cccatcttct ccagctacaa tgcccagagc
      781 gaggaacggc ggatcatcat cagatccttc ggcatcaaca gcatcaagct gcccaaggac
      841 cggatccaca gcgagaagtc caacaagagc gtggccatgg atatgctcaa cgaagtgaag
      901 cggtgccccg acgagctgtt cacaacactg tctgccgaga agcagtcccg gttcagaatc
      961 atcagcgacg accacaatga agtgctgatg aagcggagca gcgacagatt cgtgcctctg
     1021 ctgctgcagt atatcgatta cggcaagctg ttcgaccaca tcaggttcca cgtgaacatg
     1081 ggcaagctga gatacctgct gaaggccgac aagacctgca tcgacggcca gaccagagtc
     1141 agagtgatcg agcagcccct gaacggcttc ggcagactgg aagaggccga gacaatgcgg
     1201 aagcaagaga acggcacctt cggcaacagc ggcatccgga tcagagactt cgagaacatg
     1261 aagcgggacg acgccaatcc tgccaactat ccctacatcg tggacaccta cacacactac
     1321 atcctggaaa acaacaaggt cgagatgttt atcaacgaca aagaggacag cgccccactg
     1381 ctgcccgtga tcgaggatga tagatacgtg gtcaagacaa tccccagctg ccggatgagc
     1441 accctggaaa ttccagccat ggccttccac atgtttctgt tcggcagcaa gaaaaccgag
     1501 aagctgatcg tggacgtgca caaccggtac aagagactgt tccaggccat gcagaaagaa
     1561 gaagtgaccg ccgagaatat cgccagcttc ggaatcgccg agagcgacct gcctcagaag
     1621 atcctggatc tgatcagcgg caatgcccac ggcaaggatg tggacgcctt catcagactg
     1681 accgtggacg acatgctgac cgacaccgag cggagaatca agagattcaa ggacgaccgg
     1741 aagtccattc ggagcgccga caacaagatg ggaaagagag gcttcaagca gatctccaca
     1801 ggcaagctgg ccgacttcct ggccaaggac atcgtgctgt ttcagcccag cgtgaacgat
     1861 ggcgagaaca agatcaccgg cctgaactac cggatcatgc agagcgccat tgccgtgtac
     1921 gatagcggcg acgattacga ggccaagcag cagttcaagc tgatgttcga gaaggcccgg
     1981 ctgatcggca agggcacaac agagcctcat ccatttctgt acaaggtgtt cgcccgcagc
     2041 atccccgcca atgccgtcga gttctacgag cgctacctga tcgagcggaa gttctacctg
     2101 accggcctgt ccaacgagat caagaaaggc aacagagtgg atgtgccctt catccggcgg
     2161 gaccagaaca agtggaaaac acccgccatg aagaccctgg gcagaatcta cagcgaggat
     2221 ctgcccgtgg aactgcccag acagatgttc gacaatgaga tcaagtccca cctgaagtcc
     2281 ctgccacaga tggaaggcat cgacttcaac aatgccaacg tgacctatct gatcgccgag
     2341 tacatgaaga gagtgctgga cgacgacttc cagaccttct accagtggaa ccgcaactac
     2401 cggtacatgg acatgcttaa gggcgagtac gacagaaagg gctccctgca gcactgcttc
     2461 accagcgtgg aagagagaga aggcctctgg aaagagcggg cctccagaac agagcggtac
     2521 agaaagcagg ccagcaacaa gatccgcagc aaccggcaga tgagaaacgc cagcagcgaa
     2581 gagatcgaga caatcctgga taagcggctg agcaacagcc ggaacgagta ccagaaaagc
     2641 gagaaagtga tccggcgcta cagagtgcag gatgccctgc tgtttctgct ggccaaaaag
     2701 accctgaccg aactggccga tttcgacggc gagaggttca aactgaaaga aatcatgccc
     2761 gacgccgaga agggaatcct gagcgagatc atgcccatga gcttcacctt cgagaaaggc
     2821 ggcaagaagt acaccatcac cagcgagggc atgaagctga agaactacgg cgacttcttt
     2881 gtgctggcta gcgacaagag gatcggcaac ctgctggaac tcgtgggcag cgacatcgtg
     2941 tccaaagagg atatcatgga agagttcaac aaatacgacc agtgcaggcc cgagatcagc
     3001 tccatcgtgt tcaacctgga aaagtgggcc ttcgacacat accccgagct gtctgccaga
     3061 gtggaccggg aagagaaggt ggacttcaag agcatcctga aaatcctgct gaacaacaag
     3121 aacatcaaca aagagcagag cgacatcctg cggaagatcc ggaacgcctt cgatcacaac
     3181 aattaccccg acaaaggcgt ggtggaaatc aaggccctgc ctgagatcgc catgagcatc
     3241 aagaaggcct ttggggagta cgccatcatg aagtaaggcc gcactcgagg cccgaaagga
     3301 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     3361 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc ccgcaagagg
     3421 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
     3481 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
     3541 tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg
     3601 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3661 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3781 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3841 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3901 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3961 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4021 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     4081 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4141 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4201 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4261 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4321 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     4381 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4441 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4501 tggagccggt gagcgtgggt cacgcggtat cattgcagca ctggggccag atggtaagcc
     4561 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4621 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4681 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4741 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4981 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5221 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5521 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5581 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     5641 cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5701 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     5761 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5821 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5881 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
     5941 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     6001 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     6061 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     6121 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     6181 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     6241 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     6301 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     6361 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     6421 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     6481 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg
     6541 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     6601 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     6661 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
     6721 ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct
     6781 cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca atgatcgaag ttaggctggt
     6841 aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     6901 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     6961 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat
     7021 gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc
     7081 ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct
     7141 ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag
     7201 ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg
     7261 gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg
     7321 agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7381 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7441 gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg
     7501 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
     7561 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc
     7621 ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc
     7681 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca
     7741 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg
     7801 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga
     7861 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct
     7921 ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt
     7981 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat
     8041 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt
     8101 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg
     8161 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg
     8221 ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca
     8281 cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg
     8341 cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat
     8401 actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt
     8461 ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct
     8521 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     8581 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
     8641 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     8701 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     8761 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
     8821 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
     8881 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
     8941 agcggcctgg tgccgcgcgg cagccatatg gctagctgga gccatccgca gtttgaaaaa
     9001 ggtggtggta gcggtggtgg ttcaggtggt agtgcatggt cacaccctca gtttgagaaa
     9061 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct
     9121 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa
     9181 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg
     9241 gactccttaa gattcttgta cgacggtatt agaatccaag ctgatcagac ccctgaagat
     9301 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tgga
//
