LOCUS       sequence-223030-        2653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Omega 2 destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: Omega 2
FEATURES             Location/Qualifiers
     source          1..2653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..81
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        135..153
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     135..152
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    160..216
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(218..237)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(219..237)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(244..261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(244..260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(253..275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(469..488)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(620..1208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(700..719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1323..1425
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1426..2085
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1492..1511)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2198..2626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2285..2304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2495..2516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taactgcagg tctcagtcag gagagagacg cctgttgaca attaatcatc ggcatagtat
       61 atcggcatag tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgaccat
      121 gattacgcca agctatttag gtgacactat agaatactca agcttgcatg cctgcaggtc
      181 gactctagag gatccccggg taccgagctc gaattcgccc tatagtgagt cgtattacaa
      241 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      301 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      361 tcgcccttcc caacagttgc gcagcctgaa tggcgaatga cgcctgatgc ggtattttct
      421 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      481 tgatgccgca tagcgtctct cgcttgagac ctgcagtgac acggttatcc acagaatcag
      541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1021 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgt
     1321 gatgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1381 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1441 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1501 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1561 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1621 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     1681 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1741 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1801 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtg
     1861 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1921 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1981 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2041 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     2101 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     2161 ggcagaaatt cgatgataag ctgtcaaaca tgatgttacg cgccctgtag cggcgcatta
     2221 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2281 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2341 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2401 aaaaaacttg attagggtga tggttcacgt agtgggccag cgccctgata gacggttttt
     2461 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2521 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2581 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2641 acgcttacaa ttt
//
