LOCUS       sequence-223033-        2860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - NLuc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pNLuc
FEATURES             Location/Qualifiers
     source          1..2860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(662..680)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(663..680)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(696..712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(696..712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(827..1415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(907..926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1530..1632
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1633..2292
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1699..1718)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2405..2833
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2492..2511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2702..2723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaagctc aggcgagtat
      121 tcgttcgcag agcctccacc ctgttacgcc gtttatcgtt acgcggaaaa gcaaagatcc
      181 gtctggattg atcaatcgct cgtcaataat cttgttgccg ttccagaggg ttccagtgac
      241 ggtgattttc ttgccgtcga atacagcgat cccctcgtaa ggtcggccaa agtaatctat
      301 catattagga gtaactccat cgattaccaa agtcccataa tgaaggataa ctttgaagtg
      361 atgatcatca acgggataaa ctactttgaa tattttttca atctgaccca tttggtcccc
      421 agacagtcct tcatacggga ttatgacgtg gatatctatt ttcaatccgt tctctccgga
      481 aaggacaatt ctctgaatag gggtaacgga cacgcccaag ttttggaaaa ggctgctgac
      541 tcctccctgt tcgaggacct ggtccaaatt gtatcctgca gtttgcctcc agtcacccac
      601 gaagtcctcc aaggtaaaca ccattcgaga ccactcatcg cccaatcact agtgaattcg
      661 ttctatagtg tcacctaaat accatagagc cgttggtcat agctgtttcc tgtccaggca
      721 tcatgacacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1261 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1441 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1501 aaaggatctt cacctagatc cttttgtgat gatcggcacg taagaggttc caactttcac
     1561 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1621 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1681 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1741 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1801 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     1861 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1921 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1981 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     2041 gggtttattg agaatatgtt tttcgtgtca gccaatccct gggtgagttt caccagtttt
     2101 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     2161 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     2221 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     2281 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
     2341 tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga
     2401 tgttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2461 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2521 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2581 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2641 gggccagcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2701 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2761 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2821 tttaacgcga attttaacaa aatattaacg cttacaattt
//
