LOCUS       sequence-223035-        3280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Renilla Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pRenilla
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1082..1100)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1083..1100)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1116..1132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1116..1132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1247..1835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1327..1346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1950..2052
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2053..2712
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2119..2138)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2825..3253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2912..2931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3122..3143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaagctc actgttcgtt
      121 cttcaggacc ctctctacaa aggactttat gtatttgccc atctcgtctg gagcgtcctc
      181 ttgactgaaa tgcagtccct ttactttgac aaattcggta tttggaaatt tctttgctcc
      241 ttctacgata gcgttgctaa aaaatcctgg atctgattct ataaacattt tagggagatc
      301 gtcagaggcc cggaggtacg cgttgtaatt tcggactatt tgcacaacat ccggctttcc
      361 gcctttgacg agcgggattt ctcttggcca ggaaagtgtt ggcctgcgga cttctccctt
      421 ttctttaaac ggttcgagat aagcggcaaa ttcctcgggt tccagttttc tcatgatttt
      481 tgaaggaagc attgtttcca caaagaagtt gttctccagc accatctttt ccccttcctc
      541 gcttttaatc aatgctatat cctcctcaat atctggccat tcgtcccagg actcgattac
      601 atctactaca gactcggcgt gaacgattgc ctttatcttg tcctgatgct cgtaggaata
      661 atggaacgcg agacaggctc cccaatcgtg acccacgaag atgattttct tcggcaaatt
      721 caagagttcg aaccaagccg tcagatactt gtagtggtca agaaggcgat aagatccatt
      781 ccctgacttg ccactctttc ccattccgat cagatcaggg ataatacacc tagctaccgg
      841 ctctatatgc gggactacgt gtcgccacaa ataagaggag gctgcgttgc catgcaaaaa
      901 aattacggcg ttttctgcat gcttttcact gtcgtagtaa ttgatgaaac tatcaaggac
      961 attcatttgt ttacatctgg cccaccactg aggcccagtt atcattctct tgcgttgctc
     1021 ggggtcataa accttggaag tcattcgaga ccactcatcg cccaatcact agtgaattcg
     1081 ttctatagtg tcacctaaat accatagagc cgttggtcat agctgtttcc tgtccaggca
     1141 tcatgacacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1681 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1921 aaaggatctt cacctagatc cttttgtgat gatcggcacg taagaggttc caactttcac
     1981 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     2041 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     2101 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     2161 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     2221 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     2281 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     2341 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     2401 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     2461 gggtttattg agaatatgtt tttcgtgtca gccaatccct gggtgagttt caccagtttt
     2521 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     2581 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     2641 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     2701 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
     2761 tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga
     2821 tgttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3061 gggccagcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3241 tttaacgcga attttaacaa aatattaacg cttacaattt
//
